Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Effects of bis(2-ethylhexyl) phthalate(DEHP) on plant soil-borne pathogenic bacterium Pectobacterium carotovorum in vitro

Bis(2-ethylhexyl) phthalate가 in vitro에서 식물 토양병원성 세균 Pectobacterium carotovorum에 미치는 영향

  • Yu-Ri Kim (Division of Agricultural Microbiology, National Institute of Agricultural Sciences) ;
  • Sang Tae Kim (Division of Agricultural Microbiology, National Institute of Agricultural Sciences) ;
  • Mee Kyung Sang (Division of Agricultural Microbiology, National Institute of Agricultural Sciences)
  • 김유리 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 김상태 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 상미경 (농촌진흥청 국립농업과학원 농업미생물과)
  • Received : 2022.10.11
  • Accepted : 2022.11.04
  • Published : 2022.12.31
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Abstract

Bis(2-ethylhexyl) phthalate (DEHP) is one of the plasticizers used in the polyvinyl chloride(PVC) industry. It is known to be easily released into the environment. In this study, we investigated effects of DEHP on growth, metabolic pathway, and virulence gene expression in soil-borne bacterial plant pathogen, Pectobacterium carotovorum SCC1 using in vitro assays. As a result, DEHP at 20 ㎍ mL-1 did not affect the growth, cell membrane permeability, or ATPase activity of P. carotovorum SCC1. However, it decreased succinyl-CoA synthase (SCS) activity in the tricarboxylic acid (TCA) cycle. Relative expression levels of virulence genes encoding pectate lyase and pectin were differentially influenced by DEHP treatment. These results suggest that biological characteristics of P. carotovorum might be influenced by DEHP in soil.

본 연구는 플라스틱 가소제인 DEHP가 식물 병원균 중 하나인 P. carotovorum SCC1 균주에 미치는 영향을 조사하였다. DEHP가 균주 생장과 대사에 미치는 영향을 조사한 결과, 개체군 변화에 유의한 영향을 주지 않았으며, 세포막 투과성, ATPase 활성에 유의한 변화가 없었지만 TCA cycle 에서 DEHP 첨가에 따라 Succinyl-CoA synthase 활성이 유의적으로 감소하였다. 병원성 관련 유전자 발현량을 관찰한 결과 pectate lyase 유전자 발현량이 상대적으로 증가한 반면, pectinase 유전자는 상대적으로 발현량이 감소하였다. 따라서 DEHP는 P. carotovorum SCC1의 개체군 변화나 대사에는 유의미한 영향을 미치지 않지만 병원성 관련 유전자 발현에 영향을 미치므로 본 연구 결과는 향후 실제 식물 재배 조건에서 DEHP가 존재할 때 P. carotovorum의 특성에 관한 기초연구 자료로 활용할 수 있을 것이라 사료된다.

Keywords

Acknowledgement

본 연구는 국립농업과학원 연구개발사업(과제번호: PJ01475802)의 지원에 의해 수행되었음.

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