Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
권호 표지
DOI QR코드

DOI QR Code

Assessment of the resistance of bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum KACC 21701 in Kimchi cabbage genetic resources

  • Parthiban Subramanian (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Ho Chul Ko (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Seong-Hoon Kim (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Jae Eun Lee (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Aejin Hwang (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Bichsaem Kim (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Yoon-Jung Lee (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Awraris Derbie Assefa (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Onsook Hur (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Nayoung Ro (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Jung Sook Sung (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Ju Hee Rhee (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Ho-Sun Lee (International Technology Cooperation Center, Rural Development Administration) ;
  • Bum-Soo Hahn (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration)
  • Received : 2022.10.06
  • Accepted : 2022.11.28
  • Published : 2022.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Bacterial phytopathogen Pectobacterium causes soft rot disease in several vegetable crops globally, resulting in heavy agricultural losses at both the pre and postharvest stages. The present work was carried out to screen Kimchi cabbage genetic resources conserved at the National Agrobiodiversity Center, Rural Development Administration, Korea, for resistance against the soft rot pathogen Pectobacterium carotovorum subsp. carotovorum KACC 21701 over a period of three years (from 2020 to 2022). Infection of the phytopathogen was carried out at four-leaf stage and for each accession, twenty-five plants per germplasm were infected with KACC 21701. Kimchi cabbage cultivars Wangmatbaechu, Seoulbaechu, and CR Kiyoshi were used as control. Seven-days post-infection, the Disease Index (DI) values were manually recorded from zero to four, zero matched perfectly heathy plants and four completely dead plants. The 682 accessions of Kimchi cabbage exhibited varying degrees of disease resistance to KACC 21701 and thirty accessions, exhibiting a DI≤2, were considered for replication studies. During the replication studies, four landrace germplasms (IT102883, IT120036, IT120044, and IT120048) and one cultivar(IT187919) were confirmed to be moderately susceptible to KACC 21701. Results of the preliminary screening as well as replication studies were documented for the all the 682 germplasms. Addition of such information to the passport data of stored germplasms might serve as potential bio-resource for future breeders and researchers to develop resistant varieties or study the mechanisms involved in resistance of plants to such phytopathogen.

Keywords

Acknowledgement

The study was supported by the grant (PJ01450101) funded by the National Agrobiodiversity Center, Rural Development Administration, Republic of Korea. This study was also supported by the 2022 Postdoctoral Fellowship Program(P.S.) of the National Institute of Agricultural Sciences, RDA, Republic of Korea.

References

  1. Afroz T, O Hur, N Ro, JE Lee, A Hwang, B Kim, DA Awraris, JH Rhee, JS Sung, HS Lee and BS Hahn. 2021. Evaluation of bioassay methods to assess bacterial soft rot resistance in radish cultivars. J. Life Sci. 31:609-616. http://doi.org/10.5352/JLS.2021.31.7.609
  2. Chung EK, XZ Zhang, BR Choi, EJ Lee, YR Yeoung and BS Kim. 2003. Screening of disease resistance of chinese cabbage cultivars and lines to bacterial soft rot. Res. Plant Dis. 9:39-41. https://doi.org/10.5423/RPD.2003.9.1.039
  3. Davelos AL, HM Alexander and NA Slade. 1996. Ecological genetic interactions between a clonal host plant (Spartina pectinata) and associated rust fungi Puccinia seymouriana and Puccinia sparganioides. Oecologia 105:205-213. https://doi.org/10.1007/BF00328548
  4. Davidsson P, T Kariola, O Niemi and E Palva. 2013. Pathogenicity of and plant immunity to soft rot pectobacteria. Front. Plant Sci. 4:191. https://doi.org/10.3389/fpls.2013.00191
  5. Fordham R and P Hadley. 2003. Vegetables of temperate climates: oriental Brassicas. pp. 5938-5941 In: Encyclopedia of Food Sciences and Nutrition (2nd edition) (Caballero B, ed.). Academic Press. Oxford, UK. https://doi.org/10.1016/B0-12-227055-X/01237-2
  6. Hwang BH, H Bae, HS Lim, KB Kim, SJ Kim, MH Im, BS Park, DS Kim and J Kim. 2010. Overexpression of polygalacturonase-inhibiting protein 2(pgip2) of chinese cabbage(Brassica rapa ssp. pekinensis) increased resistance to the bacterial pathogen Pectobacterium carotovorum ssp. carotovorum. Plant Cell Tiss. Org. 103:293-305. https://doi.org/10.1007/s11240-010-9779-4
  7. Jeon YA, JH Rhee, JE Lee, JS Sung, OS Hur, NY Ro, SY Lee and HS Lee. 2018. Screening of disease resistance of introduced lettuce cultivars from overseas to fusarium wilt(Fusarium oxysporum f. Sp. Lactucae). J. Kor. Soc. Int. Agric. 30:197-204. https://doi.org/10.12719/KSIA.2018.30.3.197
  8. Kahlon PS, M Verin, R Huckelhoven and R Stam. 2021. Quantitative resistance differences between and within natural populations of Solanum chilense against the oomycete pathogen Phytophthora infestans. Ecol. Evol. 11:7768-7778. https://doi.org/10.1002/ece3.7610
  9. Kim JH, HJ Park, GH Nam, KU Lee and JS Kim. 2019. Floristic study of Mt. Jaamsan in the Gyeongsang supergroup, Korea. Korean J. Environ. Biol. 37:229-248. https://doi.org/10.11626/KJEB.2019.37.3.229
  10. Kim SG, GT Cho, JY Yi, YY Lee, HC Ko, JR Lee, YW Na, HJ Park and JG Gwag. 2016. Screening of soft rot resistance accessions in lettuce germplasm. J. Kor. Soc. Int. Agric. 28:92-100. https://doi.org/10.12719/KSIA.2016.28.1.92
  11. Ko HR, EH Park, EH Kang, HW Choi, Y Lee, J Kim and SK Hong. 2021a. Effects of Heterodera trifolii on Fusarium wilt disease in radish plants. Korean J. Environ. Biol. 39:1-5. https://doi.org/10.11626/KJEB.2021.39.1.001
  12. Ko HR, E Park, EH Kim, SK Park, H Kang and B Park. 2021b. Screening for resistance of Brassicaceae plant resources to clover cyst nematode. Korean J. Environ. Biol. 39:329-335. https://doi.org/10.11626/KJEB.2021.39.3.329
  13. Lee DH, JA Lim, J Lee, E Roh, K Jung, M Choi, C Oh, S Ryu, J Yun and S Heu. 2013. Characterization of genes required for the pathogenicity of Pectobacterium carotovorum subsp. carotovorum PCC21 in chinese cabbage. Microbiology-UK 159:1487-1496. https://doi.org/10.1099%2Fmic.0.067280-0 https://doi.org/10.1099%2Fmic.0.067280-0
  14. Lee SM, YH Choi, KS Jang, H Kim, SW Lee and GJ Choi. 2018. Development of an efficient bioassay method for testing resistance to bacterial soft rot of radish. Plant Dis. Res. 24:193-201. https://doi.org/10.5423/RPD.2018.24.3.193
  15. Lee SM, YH Choi, KS Jang, H Kim, HT Kim and GJ Choi. 2020. Development of an efficient bioassay method for testing resistance to bacterial soft rot of chinese cabbage. Plant Dis. Res. 26:159-169. https://doi.org/10.5423/RPD.2020.26.3.159
  16. Leite LN, EG de Haan, M Krijger, P Kastelein, PS van der Zouwen, GW van den Bovenkamp, ND Tebaldi and JM van der Wolf. 2014. First report of potato blackleg caused by Pectobacterium carotovorum subsp. brasiliensis in the Netherlands. New Dis. Rep. 29:24-24. https://doi.org/10.5197/j.2044-0588.2014.029.024
  17. Liu H, M Zhou, L Yang, W Luo, S Che, J Su, B Zhao, Y Lu, B Hu and J Fan. 2019a. First report of Pectobacterium carotovorum subsp. brasiliense causing soft rot on Raphanus sativus in China. Plant Dis. 103:1409-1409. https://doi.org/10.1094/PDIS-11-18-2107-PDN
  18. Liu M, F Wu, S Wang, Y Lu, X Chen, Y Wang, A Gu, J Zhao and S Shen. 2019b. Comparative transcriptome analysis reveals defense responses against soft rot in chinese cabbage. Hort. Res. 6:68. https://doi.org/10.1038/s41438-019-0149-z
  19. Mansfield J, S Genin, S Magori, V Citovsky, M Sriariyanum, P Ronald, M Dow, V Verdier, SV Beer, MA Machado, I Toth, G Salmond and GD Foster. 2012. Top 10 plant pathogenic bacteria in molecular plant pathology. Mol. Plant Pathol. 13:614-629. https://doi.org/10.1111/j.1364-3703.2012.00804.x
  20. Meng X, A Chai, Y Shi, X Xie, Z Ma and B Li. 2017. Emergence of bacterial soft rot in cucumber caused by Pectobacterium carotovorum subsp. brasiliense in China. Plant Dis. 101:279-287. https://doi.org/10.1094/pdis-05-16-0763-re
  21. Okon S, T Ociepa, A Nucia, M Cieplak and K Kowalczyk. 2021. Is every wild species a rich source of disease resistance? Avena fatua L.-Potential donor of resistance to powdery mildew. Plants 10:560. https://doi.org/10.3390%2plants10030560 https://doi.org/10.3390%2plants10030560
  22. Pathirana R and F Carimi. 2022. Management and utilization of plant genetic resources for a sustainable agriculture. Plants 11:2038. https://doi.org/10.3390/plants11152038
  23. Savary S, L Willocquet, SJ Pethybridge, P Esker, N McRoberts and A Nelson. 2019. The global burden of pathogens and pests on major food crops. Nat. Ecol. Evol. 3:430-439. https://doi.org/10.1038/s41559-018-0793-y
  24. Schroder R and R Prasse. 2013. Do cultivated varieties of native plants have the ability to outperform their wild relatives? PLoS One 8:e71066. https://doi.org/10.1371/journal.pone.0071066
  25. Weise S, U Lohwasser and M Oppermann. 2020. Document or lose it-on the importance of information management for genetic resources conservation in genebanks. Plants 9:1050. https://doi.org/10.3390/plants9081050