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아로니아 첨가 배지 내 느타리(Pleurotus ostreatus) 균사체 생장 및 항산화 효과

Mycelial growth and antioxidant effects of Pleurotus ostreatus in medium supplemented with Aronia berries

  • 최성주 (공주대학교 식물자원학과) ;
  • 편하영 (공주대학교 식물자원학과) ;
  • 박태민 (공주대학교 식물자원학과) ;
  • 박윤진 (두과녹비자원연구센터) ;
  • 오태석 (공주대학교 식물자원학과) ;
  • 조용구 (공주대학교 식물자원학과) ;
  • 장명준 (공주대학교 식물자원학과)
  • Choi, Seong-Ju (Department of Plant Resources, Kongju National University) ;
  • Pyeon, Ha-Young (Department of Plant Resources, Kongju National University) ;
  • Park, Tae-Min (Department of Plant Resources, Kongju National University) ;
  • Park, Youn-Jin (Kongju National University Legumes Green Manure Resource Center) ;
  • Oh, Tae-Seok (Department of Plant Resources, Kongju National University) ;
  • Cho, Young-Koo (Department of Plant Resources, Kongju National University) ;
  • Jang, Myoung-Jun (Department of Plant Resources, Kongju National University)
  • 투고 : 2022.07.01
  • 심사 : 2022.09.15
  • 발행 : 2022.09.30

초록

아로니아 첨가 시 생육 일자에 따른 느타리 균사체 생장량 및 pH 양상을 확인하였다. 무처리 대비 아로니아 첨가 시 균사체 직경과 건물중이 증가하는 경향을 보였으며, pH의 경우 아로니아 처리 시 배지의 농도가 낮아지는 것을 확인할 수 있었다. 아로니아 10% 첨가 배지에서 배양한 버섯 균사체와 일반 배지에서 배양한 버섯 균사체의 DPPH 라디칼 소거 활성에서 아로니아 첨가 배지 내 느타리 균사체는 61.81%, 무첨가 배지 내 느타리 균사체는 49.65%로 약 12%의 라디칼 소거 활성 차이를 보였으며, ABTS 라디칼 소거 활성은 아로니아 첨가 배지 내 느타리 균사체 59.83%, 무첨가 배지 내 느타리 균사체 52.66%로 나타나 7.17%의 라디칼 소거 활성 차이를 보였다. 총 폴리페놀 함량의 경우 아로니아 첨가 배지 내 생육 균사체 6.54 mg GAE/g, 무첨가 배지 내 생육 균사체 5.77 mg GAE/g로 0.77 mg GAE/g의 차이를 나타냈다. 생육 배지에 아로니아를 첨가함에 따라 느타리 균사체의 총 폴리페놀 함량이 많아지며 ABTS, DPPH에 의한 항산화 효과가 높아지는 경향을 나타냈다.

This study was conducted to investigate the growth and antioxidant activity of Pleurotus ostreatus mycelia grown in medium supplemented with Aronia. The diameter and dry weight of the mycelia were increased in the medium supplemented with Aronia compared with the untreated medium. The total polyphenol content of mycelia grown in medium supplemented with Aronia and untreated medium was 6.54 mg GAE/g and 5.77 mg GAE/g, respectively. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity of mycelia grown in medium supplemented with Aronia and untreated medium was 61.81% and 49.65%, respectively. Moreover, the 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity of mycelia grown in medium supplemented with Aronia and untreated medium was 59.83% and 52.66%, respectively. These results confirmed that P. ostreatus mycelial growth and antioxidant activity were increased when Aronia was added to the culture medium.

키워드

과제정보

2022년도 농촌진흥청 수출용버섯 농가경영비 절감을 위한 표준배지 개발 및 안전성 연구(과제번호 : PJ016110032022)의 지원에 의해 수행된 결과입니다.

참고문헌

  1. Barros L, Ferreira MJ, Queiros B, Ferreira ICFR, Baptista P. 2007. Total phenols, ascorbic acid, β-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chem 103: 413-419. https://doi.org/10.1016/j.foodchem.2006.07.038
  2. Bondet V, Brand-Williams W, Berset C. 1997. Kinetics and mechanisms of antioxidant activity using the DPPH free radical method. LWT 30: 609-615. https://doi.org/10.1006/fstl.1997.0240
  3. Chang ST, Buswell JA, Chiu SW. 1993. Mushroom biology and mushroom products. The Chinese University Press. Hong Kong. 3-17.
  4. Choi JI, Lee YH, Ha TM, Jeon DH, Chi JH, Shin PG. 2015. Characteristics of new mid-high temperature adaptable oyster mushroom variety Heuktari for bottle culture. J Mushrooms 13: 74-78. https://doi.org/10.14480/JM.2015.13.1.74
  5. Cho SH, Lee SD, Ryu JS, Kim NG, Lee DS. 2001. Change in quality of king oyster mushroom during modified atmosphere storage. Kor J Postharvest Sci Technol 8: 367-373.
  6. Gu L, Kelm MA, Hammerstone JF, Beecher G, Holden J, Haytowitz D, Gebhardt S, Prior RL. 2004. Concentrations of proanthocyanidins in common foods and estimations of normal consumption. J Nutr 134: 613-617. https://doi.org/10.1093/jn/134.3.613
  7. Han HA, Choi SR, Song YE, Lee SY, Shin SH, Yu YJ, Kim MK. 2020. Tannin-reducing effect and changes of physicochemical properties in Aronia homogenate after treatment with liquid cultured mushroom mycelia. J Korean Soc Food Sci Nutr 33: 339-346. https://doi.org/10.3746/JKFN.2004.33.2.339
  8. Hardin JW. 1973. The enigmatic chokeberries (Aronia, Rosaceae). Bull Torrey Bot Club 100: 178-184. https://doi.org/10.2307/2484630
  9. Jeoung YK, Jeong HK, Baek IS, Kang YJ, Jung HJ. 2017. Effect of schizandra berry dregs and rice bran treatment on γaminobutyric acid (GABA) content enhancement in Pleurotus ostreatus. J Mushrooms 15: 88-93.
  10. Jeppsson N. 1999. Genetic variation and fruit quality in sea buckthorn and black chokeberry. Ph. D. Thesis. pp. xx. Swedish University of Agricultural Sciences. Uppsala, Sweden.
  11. Jeppsson N. 2000. The effects of fertilizer rate on vegetative growth, yield and fruit quality, with special respect to pigments, in black chokeberry (Aronia melanocarpa) cv. 'Viking'. SciHortic (Amsterdam) 83: 127-137. https://doi.org/10.1016/S0304-4238(99)00070-9
  12. Kang MS, Kang TS, Kang AS, Shon HR, Sung JM. 2000. Studies on mycelial growth and artificial cultivation of Pleurotus eryngii. Kor J Mycol 28: 73-80.
  13. Kokotkiewicz A, Jaremicz Z, Luczkiewicz M. 2010. Aronia plants: a review of traditional use, biological activities, and perspectives for modern medicine. J Med Food 13: 255-269. https://doi.org/10.1089/jmf.2009.0062
  14. Lee MK, Shin DI, Park, HS. 2012. Acceleration of the mycelial growth of Trametes veriscolor by spent coffee ground. Kor J Mycol 40: 292-295. https://doi.org/10.4489/KJM.2012.40.4.292
  15. Lee CJ, Han HS, Jeon CS, Jeong JC, Oh JA, Kong WS, Park KC, Park CG, Shin SY. 2011. Development of new substrate using red ginseng marc for bottle culture of oyster mushroom (Pleurotus ostreatus). J Mushrooms 9: 139-144.
  16. Ma TW, Lai Y, Yang FC. 2014. Enhanced production of triterpenoid in submerged cultures of Antrodia cinnamomea with the addition of citrus peel extract. Bioprocess Biosyst Eng 37: 2251-2261. https://doi.org/10.1007/s00449-014-1203-8
  17. Ministry for Agriculture, Food, Rural Affairs (MAFRA). 2020. The production performance of special crops in 2019, Republic of Korea. 64.
  18. Oszmianski J, Kucharska A. Tannins. 1995. Aronia. Scientific papers of the Agricultural University of Wroclaw. Food Technol 8: 55-65.
  19. Oszmianski J, Wojdylo A. 2005. Aronia melanocarpa phenolics and their antioxidant activity. Eur Food Res Technol 221: 809-13. https://doi.org/10.1007/s00217-005-0002-5
  20. Park YJ, Oh TS, Kim TK, Kang MK, Jang MJ. 2020. The effect of bean sprouts by-product addition on the growth of Pleurotus ostreatus. J Mushrooms 18: 141-144.
  21. Skupien K, Oszmianski J. 2007. The effect of mineral fertilization on nutritive value and biological activity of chokeberry fruit. Agric Food Sci 16: 46-55. https://doi.org/10.2137/145960607781635822