Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Characterization of Photobacterium sp. YW2207 isolated from rainbow trout (Oncorhynchus mykiss) raised in a fresh water farm in South Korea

국내 양식 무지개송어(Oncorhynchus mykiss)에서 분리된 Photobacterium sp. YW2207의 특성

  • Hyunwoo Kim (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • Eunsup Lee (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • Sung Jun Lee (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • Haneul Kim (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • So-Ra Han (Genome-based BioIT Convergence Institute) ;
  • Tae-Jin Oh (Department of Pharmaceutical Engineering and Biotechnology, Sunmoon University) ;
  • Myoung Sug Kim (Pathology Research Division, National Institute of Fisheries Science) ;
  • Soo-Jin Kim (Pathology Research Division, National Institute of Fisheries Science) ;
  • Se Ryun Kwon (Department of Aquatic Life Medical Sciences, Sunmoon University)
  • 김현우 (선문대학교 수산생명의학과) ;
  • 이은섭 (선문대학교 수산생명의학과) ;
  • 이승준 (선문대학교 수산생명의학과) ;
  • 김한을 (선문대학교 수산생명의학과) ;
  • 한소라 (유전체 기반 바이오IT 융합연구소) ;
  • 오태진 (선문대학교 제약생명공학과) ;
  • 김명석 (국립수산과학원 병리연구과) ;
  • 김수진 (국립수산과학원 병리연구과) ;
  • 권세련 (선문대학교 수산생명의학과)
  • Received : 2023.11.16
  • Accepted : 2023.12.07
  • Published : 2023.12.31
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Abstract

Photobacterium sp. YW2207 was isolated from rainbow trout raised in a fish farm located in Yeongwol-gun, Gangwon Province, South Korea. Based on 16S rRNA sequence analysis and phylogenetic analysis, it was confirmed that Photobacterium sp. YW2207 showed 100% similarity with Photobacterium piscicola and Photobacterium phosphoreum, and 94.6% similarity with P. damselae subsp. damselae. Biochemical analysis revealed that Photobacterium sp. YW2207 is a Gram-negative, motile bacterium with a cell size of 1.5~3×3~5 ㎛. The bacteria were cultured on nutrient agar, brain heart infusion agar, Muller-Hinton agar, tryptic soy agar, and thiosulfate citrate bile sucrose agar with NaCl concentrations ranging from 0 to 2.5%. The API50CHE and API20E tests indicated lower utilization capabilities compared to the P. damselae strains provided in the API database. Furthermore, unlike most Photobacterium species, Photobacterium sp. YW2207 presented negative for catalase test. Results from the flow cytometric measurement indicated that Photobacterium sp. YW2207 exhibited a more diverse distribution of cell sizes and had larger cell sizes compared with P. damselae subsp. damselae. Minimum inhibitory concentration tests showed that Photobacterium sp. YW2207 had low susceptibility to β-Lactam and aminoglycoside antibiotics, while having high susceptibility to tetracycline, doxycycline, and quinolone antibiotics. Pathogenicity on rainbow trout revealed that an immersion of 1×105 CFU/ml did not cause mortality or clinical symptoms.

Keywords

Acknowledgement

이 연구는 국립수산과학원 (R2023058, 수산분야 항생제 통합감시 및 내성균 연구)의 지원에 의해 진행되었습니다.

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