Journal of Korean Medicine for Obesity Research (한방비만학회지)

The Society of Korean Medicine for obesity Research (한방비만학회)
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Crude Extract and Solvent-Partitioned Fractions of the Halophyte Atriplex gmelinii Inhibit Adipogenesis in 3T3-L1 Preadipocytes

3T3-L1 지방전구세포에서 염생식물 Atriplex gmelinii의 조추출물과 용매 분획물의 지방세포분화 억제

  • Jung Im Lee (Incheon Regional Office, National Fishery Products Quality Management Service) ;
  • Jung Hwan Oh (Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University) ;
  • Chang-Suk Kong (Department of Food and Nutrition, Silla University) ;
  • Youngwan Seo (Division of Convergence on Marine Science, Korea Maritime and Ocean University)
  • 이정임 (국립수산물품질관리원 인천지원) ;
  • 오정환 (신라대학교 해양식의약소재융합기술연구소) ;
  • 공창숙 (신라대학교 식품영양학과) ;
  • 서영완 (한국해양대학교 해양과학융합학부)
  • Received : 2023.10.19
  • Accepted : 2023.11.29
  • Published : 2023.12.31
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Abstract

Objectives: Atriplex gmelinii C. A. Meyer is a halophyte belonging to the Chenopodiaceae family, and its young leaves and stems are used as fodder for livestock. The aim of the present study was to investigate the effects of A. gmelinii extract and its solvent fractions on lipid accumulation during adipogenesis of 3T3-L1 preadipocytes. Methods: The samples of A. gmelinii were separately extracted using methylene chloride and methanol. Subsequently, they were combined to formulate the initial extract, which was then partitioned based on polarity to prepare solvent fractions. Oil Red O staining was employed to measure lipid accumulation during the differentiation of 3T3-L1 preadipocytes. To verify cytotoxicity in 3T3-L1 cells, MTT assays were conducted. The expression levels of transcription factors in 3T3-L1 preadipocytes were measured through Western blotting analysis. Results: At 50 ㎍/mL, treatment of A. gmelinii extract and its solvent fractions during the differentiation of 3T3-L1 preadipocytes significantly diminished lipid accumulation with no noteworthy cytotoxicity on cell viability. Additionally, when investigating the biochemical pathways that underlie the prevention of lipid accumulation using solvent fractions, it was found that the n-BuOH and n-hexane fractions significantly decreased the expression of key transcription factors involved in the generation of fat, such as peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding protein α (C/EBPα), and sterol regulatory element-binding protein-1c (SREBP1c). Conclusions: These findings indicate that A. gmelinii can effectively reduce the accumulation of fat in 3T3-L1 adipocytes, making it a potentially valuable material for mitigating and preventing obesity.

Keywords

Acknowledgement

본 연구는 해양수산부 재원으로 해양수산과학기술진흥원 해양바이오전략소재개발 및 상용화지원사업(No. 20190096)과 과학기술정보통신부의 재원으로 한국연구재단의 기본 연구사업(No. 2022R1F1A1065328)의 지원을 받아 수행된 연구결과입니다.

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