한국식품저장유통학회지 (Food Science and Preservation)

  • 제30권4호
  • /
  • Pages.654-662
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  • 2023
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  • 3022-5477(pISSN)
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  • 3022-5485(eISSN)
한국식품저장유통학회 (The Korean Society of Food Preservation)
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효소처리 농도 및 시간에 따른 섬쑥부쟁이 추출물의 품질 특성

Quality characteristics of Aster glehni extract depending on the concentration and time of enzyme treatment

  • 투고 : 2023.06.24
  • 심사 : 2023.08.10
  • 발행 : 2023.08.30
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초록

본 연구는 다양한 생리적 기능성을 함유한 섬쑥부쟁이의 활용도를 높이기 위해 효소처리농도 및 처리시간에 따른 품질 변화를 조사하였다. 효소처리농도 및 시간에 따른 섬쑥부쟁이 추출물의 pH 변화는 pH 5.56-5.76으로 무처리구(pH 5.88)보다 낮아지는 경향을 나타내었으며, 가용성 고형분 함량은 처리농도 의존적으로 증가하는 경향을 보였으며, 색도 변화는 처리시간이 증가할수록 적황색으로 변화됨을 확인할 수 있었다. 추출물의 유리당 함량 중 fructose 및 sucrose 함량은 효소처리를 하지 않은 무처리구(Control)에서 7.73% 및 6.78%로 가장 높은 함량은 나타내었으며, glucose 및 maltose 함량은 3.2%의 효소처리농도 및 60분의 처리시간(C구)에서 6.91% 및 4.44%로 높은 경향을 나타내었다. 항산화 활성을 나타내는 총폴리페놀 함량은 1.6%의 효소처리농도 및 120분의 효소처리시간(E구) 7.38 mg GAE/g으로 가장 높은 함량을 보였으며, DPPH 및 ABTS 라디칼 소거활성은 3.2%의 효소처리농도 및 60분의 처리시간(C구)에서 가장 높은 활성을 나타내었다. 이상의 결과로 섬쑥부쟁이 건조 분말 소재화를 위한 효소처리농도 및 시간의 적정조건을 설정할 수 있었다.

The Aster glehni extract has many therapeutic and medicinal values. Therefore, it is essential to set appropriate conditions for enzyme treatment to efficiently extract A. glehni. In this study, changes in the quality of A. glehni extract depending on the concentration and time of enzyme treatment was investigated to increase its effective utilization. Compared to the control, the pH of the extract of A. glehni its soluble solid content increased with the enzyme treatment. The color of the A. glehni extract changed from green-yellow to reddish-yellow with the increase in treatment duration. The fructose and sucrose contents of the extract were the highest at 7.73% and 6.78%, respectively, in the control group without the enzyme treatment. Glucose and maltose contents were 6.91% and 4.44% in the C group (3.2% enzyme concentration and 60 min for enzyme treatment), respectively. Total polyphenol content, which shows antioxidant activity, was the highest at 7.38 mg GAE/g in the E group (1.6% of enzyme concentration and 120 min for enzyme treatment). 2,2-diphenylpicrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) showed the highest radical scavenging activity in the C group (3.2% of enzyme concentration and 60 min for enzyme treatment). These results enable setting appropriate conditions of enzyme treatment in terms of enzyme concentration and time for the production of dry powders using A. glehni extract.

키워드

과제정보

본 논문은 농촌진흥청공동연구사업(과제번호 PJ016990 산채류 및 베리 등 지역농산물을 이용한 고령자용 식품개발 및 효능구명)의 지원에 의해 이루어진 것이며, 그 지원에 감사드립니다.

참고문헌

  1. Amerinem MA, Ough CS. Method for Analysis of Musts and Wine. Wiley & Sons, New York, USA, p 176-180 (1958)
  2. Blois MS. Antioxidant determination by the use of a stable free radical. Nature, 26, 1199-1200 (1958) https://doi.org/10.1038/1811199a0
  3. Jang JE. Healthy Sanyacho. Nexus, Seoul, Korea, p 395 (1996)
  4. Jeong J, Lim MK, Lee S. Synergistic effects exerted by the combination of Aster glehni and vitamin B complex on cognitive improvement. J Korean Soc Food Sci Nutr, 51, 523-520 (2022)
  5. Kang HM, Oh SY, Kang HM, Kwon JH, Jeong YJ. Quality characteristics of in vitro luwak coffee produced suing enzyme and microbial complexes. Korean J food Presery, 30, 287-299 (2023) https://doi.org/10.11002/kjfp.2023.30.2.287
  6. Kang YC. Studies on the microencapsulation of extract from Aster scaber and Aster glehni by spray drying. MS Thesis, Ajou University, Korea p 48-51 (2000)
  7. Kang YC, Choi KK, Kim KH, Kim HK. Microencapsulation of Aster scaber and Aster glehni by spray drying. Korean J Food Presery, 9, 212-220 (2002)
  8. Kim CM, Sin MK, An TK, Lee KS. Dictionary of Chinese Herb. Jung-Dam Publisher, Seoul, Korea, p 1431 (1997)
  9. Kim HH, Park GH, Park KS, Lee JY, An BJ. Anti-oxidant and anti-inflammation activity of fractions from Aster glehni Fr. Schm. Kor J Microbiol Biotechnol, 38, 434-441 (2010)
  10. Kim HK, Choi YJ, Kim KH. Functional activities of microwave-assisted extracts from Flammulina velutipes. Korean J Food Sci Technol, 34, 1013-1017 (2002)
  11. Korea Food Industry Association. Food Industry Exhibition. MoonYoung-sa, p 616-617 (2008)
  12. Lee JH, Lee SR. Analysis of phenolic substances content in Korean plant foods. Korean J Food Sci Technol, 26, 310-316 (1994)
  13. Lee JK, Kim HS, Lee SM, Park GS. Analysis of vegetation and vegetation-environment relationships in main wild vegetables of Ulleungdo in Korea. J Korean Env Res Tech, 21, 71-82 (2018)
  14. Lee JY, Choi GH, Lee KH. Physicochemical and sensory properties of red pepper extract treated with enzyme complex. Korean J Food Nutr, 28, 628-634 (2015)
  15. Lee JY, Park JY, Kim HD, Lee SE, Lee JH, Lee YJ, Seo KH. Anti-oxidant and anti-adipoyct differentiation of Aster glehni and Aster yomena. J Nutr Health, 52, 250-257 (2019) https://doi.org/10.4163/jnh.2019.52.3.250
  16. Lee KH, Jang DB, Lee JJ, Han KJ, Bae KA, Lee WJ, Kwon SY, Lee KJ. Changes in physicochemical quality of the extracts by solvents in the enzyme-treated Abeliophyllum distichum leaves. Porean J Food Nutr, 36, 42-49 (2023)
  17. Lee KH, Joo GY, Kim CY, Han KJ, Jang DB, Yun JH, Yu KW, Bae YJ. Physicochemical quality change of enzyme-treated Centella asiatica and preparation of jam using enzyme-treated Centella asiatica. Korean J Food Nutr, 34, 612-620 (2021)
  18. Lee LS, Cha HS, Park JD, Yi SH, Kim SH. High quality green tea extract production from enzyme treated fresh green tea leaves. J Korean Soc Food Sci Nutr, 37, 1025-1029 (2008)
  19. Lee MS, Chung MS. Analysis of volatile flavor components of Aster glehni. Korean J Soc Food Sci, 14, 547-552 (1998)
  20. Ministry of Food and Drug Safety (KR). Food Public Code. Ministry of Food and Drug Safety, Cheongju (2018)
  21. Nakabayashi T, Ina K, Sakata K. Chemistry and Function of Green, Black and Oolong Tea. Kogagu Press, Kawaski, Japan, p 20-51 (1994)
  22. Nugroho A, Kim MH, Choi J, Choi JS, Jung WT, Lee KT, Park HJ. Phytochemical studies of the phenolic sub-stances in Aster glehni extract and its sedative and anticon-vulsant activity. Arch Pharm Res, 35, 423-430 (2012) https://doi.org/10.1007/s12272-012-0304-7
  23. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med, 26, 1231-1237 (1999) https://doi.org/10.1016/S0891-5849(98)00315-3
  24. Seo SW. Evaluation of Korean wild vegetables' antioxidative activity and analysis on antioxidative components of Aster glehni and Saussurea grandifolia. Ph D Thesis, Keimyung University, Korea p 35-65 (2014)
  25. Son MJ. Studies on the phytochemical constituents from the ethyl acetate soluble fraction of Aster glehni. MS Thesis, Kyunghee University, Korea p 1-3 (2015)
  26. Son MJ, Jin C, Lee YS, Lee JY, Kim HJ. Characterization of caffeoylglucoside derivatives and hypouricemic activity of the ethyl acetate fraction from Aster glehni. Bull Korean Chem Soc, 36, 503-512 (2015) https://doi.org/10.1002/bkcs.10095
  27. Uleunggun. Annals of Ulleung no. 61. Available from: https://www.ulleung.go.kr/common/synap/skin/doc.html?fn=30dabbbd3016785e7d50009091c22b7e&rs=/upload/synap/202303. Accessed May 18, 2023.