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Quality characteristics of Aster glehni extract depending on the concentration and time of enzyme treatment

효소처리 농도 및 시간에 따른 섬쑥부쟁이 추출물의 품질 특성

  • Received : 2023.06.24
  • Accepted : 2023.08.10
  • Published : 2023.08.30

Abstract

The Aster glehni extract has many therapeutic and medicinal values. Therefore, it is essential to set appropriate conditions for enzyme treatment to efficiently extract A. glehni. In this study, changes in the quality of A. glehni extract depending on the concentration and time of enzyme treatment was investigated to increase its effective utilization. Compared to the control, the pH of the extract of A. glehni its soluble solid content increased with the enzyme treatment. The color of the A. glehni extract changed from green-yellow to reddish-yellow with the increase in treatment duration. The fructose and sucrose contents of the extract were the highest at 7.73% and 6.78%, respectively, in the control group without the enzyme treatment. Glucose and maltose contents were 6.91% and 4.44% in the C group (3.2% enzyme concentration and 60 min for enzyme treatment), respectively. Total polyphenol content, which shows antioxidant activity, was the highest at 7.38 mg GAE/g in the E group (1.6% of enzyme concentration and 120 min for enzyme treatment). 2,2-diphenylpicrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) showed the highest radical scavenging activity in the C group (3.2% of enzyme concentration and 60 min for enzyme treatment). These results enable setting appropriate conditions of enzyme treatment in terms of enzyme concentration and time for the production of dry powders using A. glehni extract.

본 연구는 다양한 생리적 기능성을 함유한 섬쑥부쟁이의 활용도를 높이기 위해 효소처리농도 및 처리시간에 따른 품질 변화를 조사하였다. 효소처리농도 및 시간에 따른 섬쑥부쟁이 추출물의 pH 변화는 pH 5.56-5.76으로 무처리구(pH 5.88)보다 낮아지는 경향을 나타내었으며, 가용성 고형분 함량은 처리농도 의존적으로 증가하는 경향을 보였으며, 색도 변화는 처리시간이 증가할수록 적황색으로 변화됨을 확인할 수 있었다. 추출물의 유리당 함량 중 fructose 및 sucrose 함량은 효소처리를 하지 않은 무처리구(Control)에서 7.73% 및 6.78%로 가장 높은 함량은 나타내었으며, glucose 및 maltose 함량은 3.2%의 효소처리농도 및 60분의 처리시간(C구)에서 6.91% 및 4.44%로 높은 경향을 나타내었다. 항산화 활성을 나타내는 총폴리페놀 함량은 1.6%의 효소처리농도 및 120분의 효소처리시간(E구) 7.38 mg GAE/g으로 가장 높은 함량을 보였으며, DPPH 및 ABTS 라디칼 소거활성은 3.2%의 효소처리농도 및 60분의 처리시간(C구)에서 가장 높은 활성을 나타내었다. 이상의 결과로 섬쑥부쟁이 건조 분말 소재화를 위한 효소처리농도 및 시간의 적정조건을 설정할 수 있었다.

Keywords

Acknowledgement

본 논문은 농촌진흥청공동연구사업(과제번호 PJ016990 산채류 및 베리 등 지역농산물을 이용한 고령자용 식품개발 및 효능구명)의 지원에 의해 이루어진 것이며, 그 지원에 감사드립니다.

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