Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Ethanol extract of Aster glehni exhibits anti-inflammatory and anti-oxidant effects in RAW 264.7 cells and Caenorhabditis elegans

섬쑥부쟁이 에탄올 추출물이 대식세포와 예쁜꼬마선충에서의 항염증 및 항산화 효과

  • Mi-Kyung Seo (Department of Agro-Food Resources, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Han-Na Chu (Department of Agro-Food Resources, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Da-Bin Lee (Department of Agro-Food Resources, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Haeng-Ran Kim (Department of Agro-Food Resources, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • In-Seon Hwang (Department of Agro-Food Resources, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Yong-Jin Jeong (KMF Co., Ltd) ;
  • Sung-Ran Yoon (Division of Agriculture Environment Research, Gyongsangbuk-do Agricultural Research and Extension Services) ;
  • Seok-Seong Kang (Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University) ;
  • Kyeong-A Jang (Department of Agro-Food Resources, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Min-Sook Kang (Department of Agro-Food Resources, National Institute of Agricultural Sciences, Rural Development Administration)
  • 서미경 (국립농업과학원 농식품자원부 식생활영양과) ;
  • 추한나 (국립농업과학원 농식품자원부 식생활영양과) ;
  • 이다빈 (국립농업과학원 농식품자원부 식생활영양과) ;
  • 김행란 (국립농업과학원 농식품자원부 식생활영양과) ;
  • 황인선 (국립농업과학원 농식품자원부 식생활영양과) ;
  • 정용진 ((주)케이엠에프) ;
  • 윤성란 (경상북도농업기술원 농업환경연구과) ;
  • 강석성 (동국대학교 식품생명공학과) ;
  • 장경아 (국립농업과학원 농식품자원부 식생활영양과) ;
  • 강민숙 (국립농업과학원 농식품자원부 식생활영양과)
  • Received : 2023.11.07
  • Accepted : 2023.11.22
  • Published : 2023.12.30
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Abstract

This study investigated the anti-oxidative and anti-inflammatory effects of Aster glehni (AG) extract in RAW 264.7 cells and Caenorhabditis elegans. The total polyphenol and flavonoid contents were higher in the ethanol extracts than in the hot water extracts. As a result of measuring the moisture contents (%) and extraction yields (%) of AG and drying A. glehni for processing (DAG), 70% ethanol, which has the highest percentage of extraction yield, was selected as the final solvent. DPPH radical scavenging activity showed higher antioxidant activity of ethanol extracts of DAG than AG. The cytotoxicity assay of the AG or DAG ethanol extracts was treated at different concentrations (25, 50, and 100 ㎍/mL), and cell viability rates were higher than 80% at all concentrations. The LPS-stimulated nitric oxide (NO) production in RAW 264.7 was significantly reduced at all concentrations of AG and DAG groups. As a result of measuring the gene expression of iNOS, which induces NO production, the AG or DAG group decreased by 33% and 32%, compared with the phosphate buffer saline (PBS) group. Under inflammatory stress conditions, the survival rate of C. elegans treated with AG or DAG ethanol extract with LPS showed concentration-dependent improvement in survival rate compared with the PBS group. Considering these results, AG could potentially be developed as an antioxidant and anti-inflammatory functional food material.

본 연구는 울릉 섬쑥부쟁이의 생리활성을 평가하여 기능성 농식품 소재로서의 자료 제공을 목적으로 하였다. 대식세포와 선충을 활용하여 울릉 섬쑥부쟁이의 항산화 및 항염증 효과를 평가하였다. 총폴리페놀 함량과 총플라보노이드 함량은 AG와 DAG의 에탄올(70%, 100%) 용매가 열수보다 추출수율이 높았으며, 추출 조건별 항산화 활성 분석도 열수보다는 에탄올 추출물에서 가장 높았다. AG의 경우 100% 에탄올 추출물(4.50%)이, DAG의 경우 70% 에탄올 추출물(4.19%)로 수율이 가장 높았다. 항산화 성분의 추출수율과 라디컬소거 활성 결과와 식품소재 활용을 고려하여 70% 에탄올을 최종 용매로 선정하였다. 시료의 효과평가를 위해 세포독성이 없는 100 ㎍/mL를 최대 농도로 설정하여 대식세포 RAW 264.7에서의 항염증 활성을 평가하였다. LPS로 염증을 유도하고 AG와 DAG 에탄올 추출물 처리군은 LPS 단독 처리군(양성 대조군)과 비교하여 NO의 생성과 iNOS의 발현이 유의적으로 감소하였다. 선충을 이용한 AG와 DAG 에탄올 추출물의 항산화 활성 분석 결과, juglone 처리로 인한 산화적 스트레스 조건하에서 선충의 생존율 연장에는 영향을 미치지 못했다. 선충에 LPS를 처리하여 염증 스트레스 조건하에서 AG와 DAG 에탄올 추출물이 LPS 단독 처리군에 비해 24시간 후 생존율이 증가하였고, 두 시료 모두 생존율 개선 효과가 농도 의존적으로 보였다. 염증과 산화의 스트레스 조건 없이 선충의 평균 수명 기간인 20일 동안 AG와 DAG 추출물을 처리하여 항노화 효과를 평가한 결과 AG와 DAG 에탄올 추출물을 처리한 선충의 생존율 중앙값(10일)은 CON보다 높았으며, DAG 에탄올 추출물은 농도 의존적으로 생존율을 높이는 경향을 보였으나, AG 에탄올 추출물은 그렇지 않았다. AG와 DAG의 20일 생존율은 CON보다 낮거나 유사(DAG 50)하였다. 본 연구의 결과, 울릉 섬쑥부쟁이 에탄올 추출물은 in vitro에서 항산화 활성을 보였고, 염증이 유도된 대식세포에서 iNOS 유전자 발현을 억제하여 NO의 생성량이 감소하였다고 사료된다. 또한, 염증 스트레스를 유도한 선충의 생존율 개선 효과를 보였다. 이 결과는 울릉 섬쑥부쟁이의 건강기능식품 개발 시 기초자료로 활용하고, 지역 농산물의 소비 활성화에 도움이 될 수 있을 것으로 기대된다.

Keywords

Acknowledgement

본 성과물은 농촌진흥청 국립농업과학원 연구사업(과제번호: RS-2022-RD010178)의 지원에 의해 이루어진 것임.

References

  1. Choi MW, Kim JI. Anti-inflammatory effect of ethyl acetate fraction isolated from Undaria pinnatifida on lipopolysaccharides-stimulated Raw 264.7 cells. Kor J Fish Aquat Sci, 46, 384-392 (2013)
  2. Farias JG, Molina VM, Carrasco RA, Zepeda AB, Figueroa E, Letelier P, Castillo RL. Antioxidant therapeutic strategies for cardiovascular conditions associated with oxidative stress. Nutrients, 9, 966 (2017)
  3. Gu YR, Kim SW, Son YW, Hong JH. Antioxidant activities of solvent extracts from different Glehnia radix parts and their inhibitory effect against nitric oxide production in Raw 264.7 cell. Korean J Food Preserv, 24, 116-124 (2017) https://doi.org/10.11002/kjfp.2017.24.1.116
  4. Jang KA, Park YH, Hwang YJ, Kim HR. Evaluation of anti-inflammatory effect of Solanum melongena L. in LPS-stimulated Raw 264.7 cells based on the cooking method. Korean J Food Cook Sci, 37, 457-466 (2021) https://doi.org/10.9724/kfcs.2021.37.6.457
  5. Jang M, Hong E, Cheong JH, Kim GH. Antioxidative components and activity of domestic Cirsium japonicum extract. J Korean Soc Food Sci Nutr, 41, 739-744 (2012) https://doi.org/10.3746/jkfn.2012.41.6.739
  6. Jeon S, Kim B. The protective effects of polyphenol-rich black chokeberry against oxidative stress and inflammation. Korean J Food Sci Technol, 52, 138-143 (2020)
  7. Jeong JH, Han JS, Jung Y, Lee SM, Park SH, Park M, Shin MG, Kim N, Kang MS, Kim S, Lee KP, Kwon KS, Kim CA, Yang YR, Hwang GS, Kwon ES. A new AMPK isoform mediates glucose-restriction induced longevity non-cell autonomously by promoting membrane fluidity. Nature Communications, 14, 288 (2023)
  8. Ji BU, Park SM, Koo S, Lim B, Yu YB. Lifespan extension of fermented Zizyphus jujuba fruits in Caenorhabditis elegans. Korean J Acupunct, 31, 218-224 (2014) https://doi.org/10.14406/acu.2014.036
  9. Kang JW, Lee IC. Antioxidant and skin whitening effects of Jeju mango kernel extracts (Mangifera indica L. var. Irwin). J Korean Soc Food Sci Nutr, 51, 1266-1271 (2022) https://doi.org/10.3746/jkfn.2022.51.12.1266
  10. Kim EM, Kim GH. Physicochemical and sensory characteristics of Aster glehni Kimchi during storage at different fermentation temperatures. Korean J Food Cook Sci, 20, 11-16 (2004)
  11. Kim HH, Park GH, Park KS, Lee JY, An BJ. Anti-oxidant and anti-inflammation activity of fractions from Aster glehni Fr. Schm. Kor J Microbiol Biotechnol, 38, 434-441 (2010)
  12. Kim JH, An CW, Kim YJ, Noh YJ, Kim SJ, Hwang IH, Jeon H, Cha DS, Shin TY, Kim DK. Anti-oxidative effect of epimedii herba in Caenorhabditis elegans. Kor J Pharmacogn, 48, 298-303 (2017)
  13. Kim JH, Kwon KM, Yang JH, Ki BH, Kim DK. Antioxidative-activity of Phellinus baumii Pilat in Caenorhabditis elegans. Kor J Pharmacogn, 50, 299-304 (2019)
  14. Kim JH, Lee YB, Han SY, Kim SJ, Hwang IH, Kim DK. Antioxidant activity of Helianthus tuberosus L. flower in Caenorhabditis elegans. Kor J Pharmacogn, 50, 96-101 (2019)
  15. Kim KW, Shin JG, Lee DG. Isolation of herbicidal compound from Aster glehni Fr. Schm. Kor J Weed Sci, 28, 434-441 (2008)
  16. Kim MH, Nugroho A, Choi JW, Park HJ. The extract of Aster glehni leaves rich in caffeoylquinic acids prevents atherogenic index, oxidative stress, and body weight increase in high-fat diet-induced rats. Kor J Pharmacogn, 42, 54-60 (2011)
  17. Kim YJ, Kim JH, Noh YJ, Kim SJ, Hwang IH, Kim DK. Anti-oxidative effect of Salvia miltiorrhiza bunge in Caenorhabditis elegans. Kor J Pharmacogn, 49, 322-327 (2018)
  18. Lee BJ, Yoon YJ, Oh JW, Park ZW, Lee HJ, Kim YS, Cha DS, Kwon J, Oh CH, Jeon H. Lifespan Extending effects of Helianthus tuberosus linne in C. elegans. Kor J Pharmacogn, 47, 280-286 (2016)
  19. Lee CB. Coloured Flora of Korea. Hyangmoon-sa, Seoul, Korea, p 322 (2006)
  20. Lee HJ, Naranbulag B, Jeong SJ, Seo C, Lee SG. Analysis of antioxidant and anti-inflammatory effects of Mongolian wild lingonberry and blueberry, and identification of their bioactive compounds. Korean J Food Sci Technol, 54, 147-154 (2022)
  21. Lee JE, Kim AJ. Antioxidant activity, whitening and anti-wrinkle effects of leaf and seed extracts of Brassica juncea L. Czern. Asian J Beauty Cosmetol, 18, 283-295 (2020)
  22. Lee JY, Park JY, Kim HD, Lee SE, Lee JH, Lee YJ, Seo KH. Anti-oxidant and anti-adipocyte differentiation of Aster glehni and Aster yomena. J Nutr Health, 52, 250-257 (2019) https://doi.org/10.4163/jnh.2019.52.3.250
  23. Lee SY, Lee JS, Park SK. Effects of acorn powder on lifespan and a resistance to oxidative stress in Caenorhabditis elegans. J Korean Soc Food Sci Nutr, 42, 670-674 (2013) https://doi.org/10.3746/jkfn.2013.42.5.670
  24. Lee YY, Kang SA. Antioxidative and anti-inflammatory activities of Salvia plebeia R. Br extracts. Korean J Food Nutr, 33, 483-492 (2020)
  25. Lim HJ, Lee HJ, Lim MH, Jung MJ. Antioxidant activity of Korean Gomchwi (Ligularia fischen) extracts. J Korean Appl Sci Technol, 38, 1524-1532 (2021)
  26. Ma J, Xu XY, Wang RR, Yan HJ, Yao HJ, Zhang HM, Jiang SW. Xu, A. Lipopolysaccharide exposure induces oxidative damage in Caenorhabditis elegans: Protective effects of carnosine. BMC Pharmacol Toxicol, 21, 85 (2020)
  27. Ryu HS, Lee MJ, Ham JR, Choi RY, Lee HI, Lee HJ, Son YJ, Lee MK. Anti-inflammatory effects of soybean embryo extract in LPS-stimulated Raw 264.7 macrophages. J Korean Soc Food Sci Nutr, 48, 1303-1309 (2019) https://doi.org/10.3746/jkfn.2019.48.11.1303
  28. Shin SJ, Ha G, Jeong SJ, Ryu MS, Yang HJ, Kim Y, Oh S, Jeong DY. Evaluation of probiotic characteristics of Lactobacillus pentosus SRCM103472 isolated from traditional fermented food using Caenorhabditis elegans in vivo model. Korean J Microbiol, 57, 255-264 (2021)
  29. Yoon SR, So BR, Park J, Ryu JA. Changes in antioxidant activity and vitamin B2 content of Aster glehni based on blanching time. Korean J Food Preserv, 29, 1139-1149 (2022) https://doi.org/10.11002/kjfp.2022.29.7.1139