Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Effects of Horsetail, Alfalfa, Ortie, Chêne and Aleppo oak as Potential Hemostatic Agents on Laboratory Coagulation Tests

  • Sina Ahmadianfar (Department of Pharmacology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz) ;
  • Nahid Mehrabi (Department of Health Information Technology, Aja University of Medical Sciences) ;
  • Saeed Mohammadi (Cell Therapy and Hematopoietic Stem Cell Transplantation Research Center, Tehran University of Medical Sciences) ;
  • Ali Sobhanizadeh (Horticultural Science and Engineering - Horticultural Plants Breeding and Biotechnology-University of Mohaghegh Ardabili) ;
  • Alireza Moradabadi (Molecular and medicine research center, Khomein university of medical sciences) ;
  • Ali Noroozi-Aghideh (Department of Hematology, Faculty of Paramedicine, Aja University of Medical Sciences)
  • Received : 2023.01.14
  • Accepted : 2023.03.09
  • Published : 2023.03.31
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Abstract

This study investigated the effect of ethanol extracts of horsetail, alfalfa, ortie, chêne and aleppo oak on blood coagulation in vitro. Extraction was performed by the maceration method. Extracts were mixed with platelet and plasma, then prothrombin time (PT), activated partial thromboplastin time (APTT) and platelet aggregation tests were conducted. Alfalfa extract had a dose-dependent effect on the PT. Ortie, and horsetail, reduced the PT significantly compared to control group. Alfalfa, horsetail, and ortie reduced the APTT, but their effect was insignificant compared to the control group. The pooled extract showed the highest effect compared to the single extracts in a dose-dependent manner. Horsetail and alfalfa induced platelet aggregation in response to arachidonic acid but not in response to collagen. In the case of ortie, no aggregation occurred regarding the arachidonic acid, and incomplete was observed in response to collagen. Interestingly, blood clotting occurred immediately after adding the chêne, aleppo oak and the pooled extract, and therefore platelet poor plasma (PPP) and platelet rich plasma (PRP) became jelly. Generally, chêne and aleppo oak, as well as pooled extract, were more effective in inducing both primary and secondary coagulation pathways via shortening the PT and APTT, and induction of platelet aggregation.

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References

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