Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Effect of Skin Wrinkle Reduction Using Emulsions with Microbiome Extracts Selected by 3D Human Skin Equivalents

3차원 배양 인공피부를 활용한 마이크로바이옴 추출물의 선정 및 이를 함유한 에멀젼 제형의 피부주름개선 임상 평가

  • Jun Woo Lim (Department of Chemical Engineering, Soongsil University) ;
  • Yerim Kim (Department of Chemical Engineering, Soongsil University) ;
  • Jimin Jeong (Department of Chemical Engineering, Soongsil University) ;
  • Ji-Eun Kwon (Department of Chemical Engineering, Soongsil University) ;
  • Seong-Hyun Jo (Department of Chemical Engineering, Soongsil University) ;
  • Jindong Jang (ICURE Pharm. Inc.) ;
  • Junsu Park (ICURE Pharm. Inc.) ;
  • Yun-Gon Kim (Department of Chemical Engineering, Soongsil University) ;
  • Jae Hyun Jeong (Department of Chemical Engineering, Soongsil University)
  • Received : 2022.12.22
  • Accepted : 2023.03.23
  • Published : 2023.03.30
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Abstract

Recently, along with the remarkable increase in interest in microbiome cosmetics, the application of microbiome extracts in the complex efficacy as anti-aging, brightening etc. has become very important. In this study, Bifidobacterium bifidum (B. bifidum), which has excellent anti-wrinkle efficacy among the microbiome, was selected through an in vitro test using cells and 3D human skin equivalents. And the anti-wrinkle efficacy of cosmetics containing B. bifidum was evaluated by clinical test. Thereafter, the cytotoxicity, anti-oxidation, anti-inflammatory and anti-wrinkle efficacy of the of the bifida fermented filtrate were tested. An emulsion containing bifida fermented filtrate at a concentration of 5% (v/v) with no cytotoxicity and excellent efficacy was prepared with the placebo emulsion. The clinical test was conducted on a total of 21 women at 2 weeks comparing the bifida emulsion and placebo emulsion. Wrinkles around the eyes were instrumentally evaluated using Antera 3D. The wrinkle reduction rate of the Bifida emulsion group compared with the placebo emulsion group differed by 5.6%. Overall, the selection of microbiome using 3D human skin equivalents and the efficacy study of cosmetics with the microbiome extracts would be actively studied to the development of microbiome cosmetics and skin microbiome mechanisms.

마이크로바이옴의 화장품 응용에 대한 관심이 높아지고 마이크로바이옴 추출물의 복합적인 효능에 의한 피부 개선 연구가 중요해지고 있다. 본 연구에서는 세포 및 3차원 배양 인공피부를 이용한 in vitro 시험법을 통하여 마이크로바이옴 중 피부 주름 개선 효능이 뛰어난 Bifidobacterium bifidum (B. bifidum)을 선정하였고, 이의 추출물을 함유한 화장품의 피부주름개선 효능평가를 진행하였다. 이후, 비피다발효여과물 원료의 세포독성 및 항산화, 항염 및 주름개선 유효성 시험을 실시하였다. 세포독성이 없고 효능이 우수한 5% (v/v) 농도의 비피다발효여과물이 함유된 에멀젼을 제조하였고 placebo 에멀젼와 비교하여 총 21 명 여성을 대상으로 2 주간 임상시험을 수행하였다. Antera 3D를 사용하여 눈가 부위의 주름을 기기 평가하였다. 주름 개선도는 placebo 에멀젼 사용군 대비하여 주름 감소율(%)에서 5.6% 차이를 보였다. 이러한 3차원 배양 인공피부를 활용한 마이크로바이옴 추출물 선정 및 이를 함유한 화장품의 효능 연구는 다양한 효능을 가지는 마이크로바이옴 화장품의 개발 및 메커니즘 연구에 응용될 수 있을 것이라 기대된다.

Keywords

Acknowledgement

본 연구는 보건복지부 지원으로 한국보건산업진흥원(KHIDI)를 통한 피부과학 응용소재 선도기술 개발사업 (과제번호: HP20C0210) 및 한국연구재단을 통한 이공분야기초연구지원사업 (과제번호: NRF-2020R1F1A1071004)지원을 받아 수행된 연구임으로 이에 감사드립니다.

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