Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Identification of Antioxidant Activities and Stimulation of Human Keratinocytes Differentiation Effects of Syzygium claviflorum Extract

Syzygium claviflorum 추출물의 항산화 활성 및 각질형성세포 분화유도 효과

  • Gayeon Seo (Graduate School of Energy/Biotechnology, Dongseo University) ;
  • Jiyeon Moon (Department of Bio-Pharmaceutical Engineering, College of Bio-Health Convergence, Dongseo University) ;
  • Yukyung Park (Graduate School of Energy/Biotechnology, Dongseo University) ;
  • Juyeong Kim (Graduate School of Energy/Biotechnology, Dongseo University) ;
  • Hoyong Hyun (Department of Bio-Pharmaceutical Engineering, College of Bio-Health Convergence, Dongseo University) ;
  • Beomsu Jeong (Department of Bio-Pharmaceutical Engineering, College of Bio-Health Convergence, Dongseo University) ;
  • Thet Thet Mar Win (Department of Botany, University of Yangon, Myanmar) ;
  • Thant Zaw Win (Department of Botany, Hinthada University, Myanmar) ;
  • Sangho Choi (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Sangmi Eum (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Dongwon Kim (Graduate School of Energy/Biotechnology, Dongseo University)
  • 서가연 (부산 동서대학교 일반대학원 에너지/생명공학과) ;
  • 문지연 (부산 동서대학교 바이오헬스융합대학 바이오제약공학과) ;
  • 박유경 (부산 동서대학교 일반대학원 에너지/생명공학과) ;
  • 김주영 (부산 동서대학교 일반대학원 에너지/생명공학과) ;
  • 현호용 (부산 동서대학교 바이오헬스융합대학 바이오제약공학과) ;
  • 정범수 (부산 동서대학교 바이오헬스융합대학 바이오제약공학과) ;
  • ;
  • ;
  • 최상호 (한국생명공학연구원 해외생물소재센터) ;
  • 엄상미 (한국생명공학연구원 해외생물소재센터) ;
  • 김동원 (부산 동서대학교 바이오헬스융합대학 바이오제약공학과)
  • Received : 2023.01.27
  • Accepted : 2023.03.10
  • Published : 2023.03.30
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Abstract

We validated the physiological activity of Syzygium claviflorum (Roxb.) Wall. ex A.M. Cowan & Cowan (S. claviflorum) extracts (leaves, stems, fruits, and flowers) as a cosmetic ingredient. Firstly, S. claviflorum extracts removed over 80% of free radicals at various concentrations in antioxidant experiments using the DPPH and ABTS assay. In cytotoxicity experiments using human epidermal keratinocytes, S. claviflorum extracts showed low cytotoxicity. In addition, S. claviflorum extracts significantly increased the expression of keratin (KRT)1, KRT2, KRT9, KRT10, which are differentiation markers of keratinocytes, as well as genes involved in the maintenance of skin barrier function, including involucrin (IVL), loricrin (LOR), filaggrin (FLG), and claudin1 (CLDN1). In particular, the expression of FLG protein, inhibited by interleukin (IL)-4/IL-13 in atopic dermatitis, was restored by S. claviflorum extracts in in vitro experiments. Therefore, S. claviflorum extracts with excellent antioxidant efficacy and skin barrier improvement function will be useful materials for the development of future atopic dermatitis treatments and cosmetics.

사이자이지움 클래비플로룸(Syzygium claviflorum (Roxb.) Wall. ex A.M. Cowan & Cowan, S. claviflorum)의 추출물 (잎, 줄기, 열매, 꽃)의 화장품 소재로써 활용되기 위한 생리활성 능력을 검증하였다. 첫번째로, S. claviflorum 추출물은 DPPH와 ABTS assay법을 이용한 항산화 실험에서 다양한 농도로 처리한 결과, 약 80% 이상의 자유 라디칼을 제거하였다. 사람 피부 표피 각질형성세포(human epidermal keratinocytes)를 이용한 세포독성 실험에서는 S. claviflorum 추출물은 낮은 세포독성을 보였다. 또한, S. claviflorum 추출물은 각질형성세포의 분화인자 (keratin (KRT)1, KRT2, KRT9, KRT10)와 피부장벽의 기능 유지에 중요한 involucrin (IVL), loricrin (LOR), filaggrin (FLG)과 claudin1 (CLDN1) 유전자의 발현을 현저히 증가시켰다. 특히, in vitro 아토피 피부염 실험에서 interleukin (IL)-4/IL-13에 의해 억제된 FLG 단백질 발현이 S. claviflorum 추출물에 의해 회복되었다. 따라서, 뛰어난 항산화 효능과 피부장벽 개선 기능을 보유한 S. claviflorum 추출물은 향후 아토피 피부염 치료제 및 화장품 개발에 유용한 소재가 될 것이다.

Keywords

Acknowledgement

이 연구는 정부(과학기술정보통신부)의 재원으로 한국연구재단(No. 2020R1C1C1005206)과 부산광역시 및 (재)부산인재평생교육진흥원의 BB21플러스 사업(2022-0123)으로 지원된 연구임. 또한, 교육부와 한국연구재단의 재원으로 지원을 받아 수행된 대학 혁신지원사업의 연구결과임.

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