Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Virulence factors and antimicrobial resistance patterns of pathogenic Escherichia coli isolated from fecal samples of piglets

자돈 분변 유래 병원성 대장균의 병원성 인자 및 항생제 내성 양상

  • Hyun Sook Shin (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Keun-Ho Kim (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Jin Sung Seo (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Young Wook Kim (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Suk-Kyung Lim (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Byeong Yeal Jung (Bacterial Disease Division, Animal and Plant Quarantine Agency)
  • 신현숙 (농림축산검역본부 세균질병과) ;
  • 김근호 (농림축산검역본부 세균질병과) ;
  • 서진성 (농림축산검역본부 세균질병과) ;
  • 김영욱 (농림축산검역본부 세균질병과) ;
  • 임숙경 (농림축산검역본부 세균질병과) ;
  • 정병열 (농림축산검역본부 세균질병과)
  • Received : 2023.02.16
  • Accepted : 2023.03.16
  • Published : 2023.03.30
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Abstract

Pathogenic Escherichia coli is the cause of a wide range of diseases in pigs, including diarrhea, edema disease, and septicemia. Diarrhea caused E. coli may result in significant economic losses, making pathogenic E. coli an important pathogen for the swine industry. This study investigated the prevalence of virulence factor genes, antimicrobial resistance phenotypes, and resistance genes in E. coli isolated from feces of piglets in Korea between 2017 and 2020. As a result, 119 pathogenic E. coli isolates were obtained from 601 fecal samples. The F4 adhesin gene and the STb enterotoxin gene were commonly present in E. coli isolated from diarrhea samples. The dominant virulotypes of isolates from diarrhea samples were STb, Stx2e, and F4:LT:STb. More than 80% of the screened isolates were resistant to ampicillin, sulfisoxazole, chloramphenicol, or tetracycline. To confirm the resistance mechanisms for β-lactam or quinolone, we investigated the genotypic factors of resistance. Each of the ceftiofur-resistant E. coli produced an extended-spectrum β-lactamase encoded by blaCTX-M-14, blaCTX-M-27, and blaCTX-M-55. And all ciprofloxacin-resistant E. coli harbored mutations in quinoloneresistance-determining-regions. In addition, some of the ciprofloxacin-resistant E. coli contained the plasmid-mediated-quinolone-resistance genes such as qepA, qnrB1, or qnrD. This study has confirmed that the F4 fimbria and the STb enterotoxin are the most predominant in pathogenic E. coli isolated from piglets with diarrhea in Korea and there is a great need for responsible and prudent use of antimicrobials to treat colibacillosis.

Keywords

Acknowledgement

본연구는 농림축산검역본부의 농림축산검역검사기술개발사업(B-1543081-2021-23-01)의 연구비 지원에 의해 수행되었다.

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