Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cloning and Biochemical Characterization of a Hyaluronate Lyase from Bacillus sp. CQMU-D

  • Lu Wang (Department of Pathogen Biology, College of Basic Medical Science, Chongqing Medical University) ;
  • Qianqian Liu (Department of Pathogen Biology, College of Basic Medical Science, Chongqing Medical University) ;
  • Xue Gong (Department of Pathogen Biology, College of Basic Medical Science, Chongqing Medical University) ;
  • Wenwen Jian (Department of Pathogen Biology, College of Basic Medical Science, Chongqing Medical University) ;
  • Yihong Cui (Department of Pathogen Biology, College of Basic Medical Science, Chongqing Medical University) ;
  • Qianying Jia (Department of Infectious Diseases, The First Affiliated Hospital of Chongqing Medical University) ;
  • Jibei Zhang (International Medical College, Chongqing Medical University) ;
  • Yi Zhang (International Medical College, Chongqing Medical University) ;
  • Yanan Guo (Department of Pathogen Biology, College of Basic Medical Science, Chongqing Medical University) ;
  • He Lu (Department of Pathogen Biology, College of Basic Medical Science, Chongqing Medical University) ;
  • Zeng Tu (Department of Pathogen Biology, College of Basic Medical Science, Chongqing Medical University)
  • Received : 2022.09.23
  • Accepted : 2022.11.29
  • Published : 2023.02.28
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Abstract

Hyaluronidase (HAase) can enhance drug diffusion and dissipate edema by degrading hyaluronic acid (HA) in the extracellular matrix into unsaturated HA oligosaccharides in mammalian tissues. Microorganisms are recognized as valuable sources of HAase. In this study, a new hyaluronate lyase (HAaseD) from Bacillus sp. CQMU-D was expressed in Escherichia coli BL21, purified, and characterized. The results showed that HAaseD belonged to the polysaccharide lyase (PL) 8 family and had a molecular weight of 123 kDa. HAaseD could degrade chondroitin sulfate (CS) -A, CS-B, CS-C, and HA, with the highest activity toward HA. The optimum temperature and pH value of HAaseD were 40℃ and 7.0, respectively. In addition, HAaseD retained stability in an alkaline environment and displayed higher activity with appropriate concentrations of metal ions. Moreover, HAaseD was an endolytic hyaluronate lyase that could degrade HA to produce unsaturated HA oligosaccharides. Together, our findings indicate that HAaseD from Bacillus sp. CQMU-D is a new hyaluronate lyase and with excellent potential for application in industrial production.

Keywords

Acknowledgement

We thank Dr. Kim Hayer (Lecturer Medical Medical School, University of Leicester) for her critical reading and editing of this manuscript. This research was supported by Chongqing Natural Science Foundation [No. cstc2021jcyj-msxmX0158], and Scientific and Technological Research Program of Chongqing Municipal Education Commission [No. KJQN202113201].

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