Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Inhibitory Effect of Hirudo on Neuroinflammation in LPS-stimulated Microglial Cells

Lipopolysccharides에 의해 활성화된 미세아교세포에서 수질(水蛭) 추출물의 NF-kB 억제를 통한 뇌신경염증 억제 효과

  • Gunhyuk Park (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Sungyu Yang (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Byeong Cheol Moon (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Sumin Noh (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Hye-Sun Lim (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine)
  • 박건혁 (한국한의학연구원 한약자원연구센터) ;
  • 양선규 (한국한의학연구원 한약자원연구센터) ;
  • 문병철 (한국한의학연구원 한약자원연구센터) ;
  • 노수민 (한국한의학연구원 한약자원연구센터) ;
  • 임혜선 (한국한의학연구원 한약자원연구센터)
  • Received : 2023.02.15
  • Accepted : 2023.03.15
  • Published : 2023.04.30
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Abstract

Today, environmental pollution has been found to be one of the causes of various diseases, including brain and nervous system diseases. In particular, neurodegenerative diseases have been found to be caused by hyperactivation of immune system cells such as microglia. Preventive and therapeutic measures are needed to suppress them. Hirudo is known as a traditional herbal medicine, based on its multiple biological activities such as anti-eczema and anti-coagulation. In the present study, the anti-neuroinflammatory potential of hirudo extract was investigated in lipopolysccharide (LPS)-stimulated BV2 microglial cells and in mice. Hirudo extract significantly inhibited LPS-stimulated nitric oxide (NO) production and cytokine (IL-1Ra, KC, MCP-5, and RANTES) expression in a dose-dependent manner without causing cytotoxicity. Pretreatment with hirudo extract suppressed LPS-induced NF-κB p65 nuclear translocation. Moreover, hirudo extract reduced LPS-stimulated microglial acitivation and improved memory impairments. The results demonstrated that hirudo extract exerts anti-neuroinflammation activities, partly through inhibition of the NF-κB signaling pathway. These findings suggest that hirudo extract might have therapeutic potential with respect to neuroinflammation and neurodegenerative diseases.

Keywords

Acknowledgement

본 연구는 한국한의학연구원 '지속가능한 한약표준자원 활용기술 개발'(KSN1822320) 사업 및 한국연구재단 '미활용 생물자원의 연구기반 구축 및 활성화 연구: 동물성 자원 중심'(NRF-2020R1I1A2073003)의 지원으로 수행되었다.

References

  1. Adivi, A., Lucero, J., Simpson, N., McDonald, J. D., Lund, A. K., 2020, Exposure to traffic-generated air pollution promotes alterations in the integrity of the brain microvasculature and inflammation in female ApoE-/- mice, Toxicol. Lett., 339, 39-50. https://doi.org/10.1016/j.toxlet.2020.12.016
  2. Aminov, R., Frolov, A., Aminova, A., 2021, The influence of the planting time and conditions on the reproductive properties of Hirudo verbana and Hirudo medicinalis, Ann. Parasitol., 67, 169-174.
  3. Goshua, A., Akdis, C. A., Nadeau, K. C., 2022, World Health Organization global air quality guideline recommendations: Executive summary, Allergy., 77, 1955-1960. https://doi.org/10.1111/all.15224
  4. Han, S. H., 2019, Fine dust and dementia: Is ambient air pollution associated with cognitive health?, J. Korean. Neurol. Assoc., 37, 135-143. https://doi.org/10.17340/jkna.2019.2.3
  5. Hang, C. H., Shi, J. X., Tian, J., Li, J. S., Wu, W., Yin, H. X., 2004, Effect of systemic LPS injection on cortical NF-kappaB activity and inflammatory response following traumatic brain injury in rats, Brain. Res., 1026, 23-32. https://doi.org/10.1016/j.brainres.2004.07.090
  6. Jang, H. K., Heo, D. S., 2010, The literature review of Leech therapy, J. Pharmacopuncture., 13, 103-120. https://doi.org/10.3831/KPI.2010.13.1.103
  7. Jang, J. H., Lee, S. H., Jung, K., Yoo, H., Park, G., 2020, Inhibitory effects of myricetin on lipopolysaccharide -induced neuroinflammation, Brain. Sci., 10, 32.
  8. Karatas, E., Ceylan, M., Dernekbasi, S., 2022, Effects of mammalian blood with different glucose levels on reproduction, growth and survival of the southern medicinal leech, Hirudo verbana Carena, 1820, Anim. Reprod. Sci., 243, 107030.
  9. Lee, A. Y., Lee, J., Kim, Y. S., Choi, G., 2019, Inorganic components of insect origin medicines, Korean. Herb. Med. Inf., 7, 189-194.
  10. Lim, H. S., Moon, B. C., Lee, J., Choi, G., Park, G., 2020, The insect molting hormone 20-hydroxyecdysone protects dopaminergic neurons against MPTPinduced neurotoxicity in a mouse model of Parkinson's disease, Free. Radic. Biol. Med., 159, 23-36. https://doi.org/10.1016/j.freeradbiomed.2020.07.010
  11. Park, G., Kim, Y. U., Kusuma, I. W., 2019, Discovery of novel animal-based medicinal products with therapeutic potential in evidence-based traditional medicine, Evid. Based. Complement. Alternat. Med., 2019, 1626543.
  12. Park, G., Lee, S. H., Oh, D. S., Kim, Y. U., 2017, Melatonin inhibits neuronal dysfunction-associated with neuroinflammation by atopic psychological stress in NC/Nga atopic-like mouse models, J. Pineal. Res., 63, e12420.
  13. Tran, V. V., Park, D., Lee, Y. C., 2020, Indoor air pollution, related human diseases, and recent trends in the control and improvement of indoor air quality, Int. J. Environ. Res. Public. Health., 17, 2927.