Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Increased Production of γ-Aminobutyric Acid from Brewer's Spent Grain through Bacillus Fermentation

  • Tao Kim (Department of Food and Nutrition, Dongduk Women's University) ;
  • Sojeong Heo (Department of Food and Nutrition, Dongduk Women's University) ;
  • Hong-Eun Na (Department of Food and Nutrition, Dongduk Women's University) ;
  • Gawon Lee (Department of Food and Nutrition, Dongduk Women's University) ;
  • Jong-Hoon Lee (Department of Food Science and Biotechnology, Kyonggi University) ;
  • Ji-Yeon Kim (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Do-Won Jeong (Department of Food and Nutrition, Dongduk Women's University)
  • Received : 2022.10.28
  • Accepted : 2022.12.05
  • Published : 2023.04.28
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Abstract

Brewer's spent grain (BSG) is a waste product of the beer industry, and γ-aminobutyric acid (GABA) is a physiologically active substance important for brain and neuron physiology. In this study, we used the bacterial strains Bacillus velezensis DMB06 and B. licheniformis 0DA23-1, respectively, to ferment BSG and produce GABA. The GABA biosynthesis pathways were identified through genomic analysis of the genomes of both strains. We then inoculated the strains into BSG to determine changes in pH, acidity, reducing sugar content, amino-type nitrogen content, and GABA production, which was approximately doubled in BSG inoculated with Bacillus compared to that in uninoculated BSG; however, no significant difference was observed in GABA production between the two bacterial strains. These results provide the experimental basis for expanding the use of BSG by demonstrating the potential gain in increasing GABA production from a waste resource.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) by the Korea government (MSIT) [NRF-2022M3A9I3082364]. We thank Edanz (www.edanz.com/ac) for editing a draft of this manuscript.

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