Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Hydrogen Production from Barley Straw and Miscanthus by the Hyperthermophilic Bacterium, Cadicellulosirupter bescii

  • Minseok Cha (Research Center for Biological Cybernetics, Chonnam National University) ;
  • Jun-Ha Kim (Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University) ;
  • Hyo-Jin Choi (Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University) ;
  • Soo Bin Nho (Department of Food Science and Biotechnology, Chung-Ang University) ;
  • Soo-Yeon Kim (Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Young-Lok Cha (Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Hyoungwoon Song (Institute for Advanced Engineering) ;
  • Won-Heong Lee (Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University) ;
  • Sun-Ki Kim (Department of Food Science and Biotechnology, Chung-Ang University) ;
  • Soo-Jung Kim (Research Center for Biological Cybernetics, Chonnam National University)
  • Received : 2023.05.23
  • Accepted : 2023.06.07
  • Published : 2023.10.28
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Abstract

This work aimed to evaluate the feasibility of biohydrogen production from Barley Straw and Miscanthus. The primary obstacle in plant biomass decomposition is the recalcitrance of the biomass itself. Plant cell walls consist of cellulose, hemicellulose, and lignin, which make the plant robust to decomposition. However, the hyperthermophilic bacterium, Caldicellulosiruptor bescii, can efficiently utilize lignocellulosic feedstocks (Barley Straw and Miscanthus) for energy production, and C. bescii can now be metabolically engineered or isolated to produce more hydrogen and other biochemicals. In the present study, two strains, C. bescii JWCB001 (wild-type) and JWCB018 (ΔpyrFA Δldh ΔcbeI), were tested for their ability to increase hydrogen production from Barley Straw and Miscanthus. The JWCB018 resulted in a redirection of carbon and electron (carried by NADH) flow from lactate production to acetate and hydrogen production. JWCB018 produced ~54% and 63% more acetate and hydrogen from Barley Straw, respectively than its wild-type counterpart, JWCB001. Also, 25% more hydrogen from Miscanthus was obtained by the JWCB018 strain with 33% more acetate relative to JWCB001. It was supported that the engineered C. bescii, such as the JWCB018, can be a parental strain to get more hydrogen and other biochemicals from various biomass.

Keywords

Acknowledgement

This study was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) and Korea Smart Farm R&D Foundation (KosFarm) through Smart Farm Innovation Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) and MSIT, Rural Development Administration (RDA) (No. 421045-03). Also, this research was supported by "Regional Innovation Strategy (RIS)" through the National Research Foundation of Korea(NRF) funded by the Ministry of Education(MOE) (2021RIS-002) and the Cooperative Research Program for Agriculture Science & Technology Development (No. PJ01604601), Rural Development Administration, Republic of, Korea. This research was also supported by the Chung-Ang University Graduate Research Scholarship (Academic Scholarship for the College of Biotechnology and Natural Resources) in 2023. We also thank to Dr. Janet Westpheling at University of Georgia for providing important C. bescii mutant strains.

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