Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
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Limited in vitro differentiation of porcine induced pluripotent stem cells into endothelial cells

  • In-Won Lee (Department of Animal Bioscience, College of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Hyeon-Geun Lee (Department of Animal Bioscience, College of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Dae-Ky Moon (Department of Animal Bioscience, College of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Yeon-Ji Lee (Department of Animal Bioscience, College of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Bo-Gyeong Seo (Division of Applied Life Science, Gyeongsang National University) ;
  • Sang-Ki Baek (Gyeongsangnamdo Livestock Experiment Station) ;
  • Tae-Suk Kim (Department of Animal Bioscience, College of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Cheol Hwangbo (Division of Life Science, College of Natural Sciences, Gyeongsang National University) ;
  • Joon-Hee Lee (Department of Animal Bioscience, College of Agriculture & Life Sciences, Gyeongsang National University)
  • Received : 2023.07.27
  • Accepted : 2023.08.18
  • Published : 2023.09.30
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Abstract

Background: Pluripotent stem cells (PSCs) including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) offer the immense therapeutic potential in stem cell-based therapy of degenerative disorders. However, clinical trials of human ESCs cause heavy ethical concerns. With the derivation of iPSCs established by reprogramming from adult somatic cells through the transgenic expression of transcription factors, this problems would be able to overcome. In the present study, we tried to differentiate porcine iPSCs (piPSCs) into endothelial cells (ECs) for stem cell-based therapy of vascular diseases. Methods: piPSCs (OSKMNL) were induced to differentiation into ECs in four differentiation media (APEL-2, APEL-2 + 50 ng/mL of VEGF, EBM-2, EBM-2 + 50 ng/mL of VEGF) on cultured plates coated with matrigel® (1:40 dilution with DMEM/F-12 medium) for 8 days. Differentiation efficiency of these cells were exanimated using qRT-PCR, Immunocytochemistry, Western blotting and FACS. Results: As results, expressions of pluripotency-associated markers (OCT-3/4, SOX2 and NANOG) were higher observed in all porcine differentiated cells derived from piPSCs (OSKMNL) cultured in four differentiation media than piPSCs as the control, whereas endothelial-associated marker (CD-31) in the differentiated cells was not expressed. Conclusions: It can be seen that piPSCs (OSKMNL) were not suitable to differentiate into ECs in the four differentiation media unlike porcine epiblast stem cells (pEpiSCs). Therefore, it would be required to establish a suitable PSCs for differentiating into ECs for the treatment of cardiovascular diseases.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea funded by the Korean Government (2020R1l1A3072689), Republic of Korea. In-Won Lee, Yeon-Ji Lee and Bo-Gyeong Seo were supported by the scholarship from the BK21Plus Program, Ministry of Education, Republic of Korea.

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