Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
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Effects of Youngseonjeatonguem on Monosodium Iodoacetate-Induced Osteoarthritis in Sprague-Dawley Rats

영선제통음(靈仙除痛飮)이 Monosodium Iodoacetate로 유도한 흰쥐의 골관절염에 미치는 효과

  • Sung-Hu An (Department of Korean Medicine Rehabilitation, Gwangju Medical Center, Wonkwang University) ;
  • Yeong-jin Jeong (Department of Korean Medicine Rehabilitation, Gwangju Medical Center, Wonkwang University) ;
  • Su-Hak Kim (Department of Korean Medicine Rehabilitation, Gwangju Medical Center, Wonkwang University) ;
  • Hark-song Lee (Harksong Oriental Medical Clinic) ;
  • Seung-Ryong Yeom (Department of Korean Medicine Rehabilitation, Gwangju Medical Center, Wonkwang University) ;
  • Young-Dal Kwon (Department of Korean Medicine Rehabilitation, Gwangju Medical Center, Wonkwang University)
  • 안성후 (원광대학교 광주한방병원 한방재활의학과) ;
  • 정영진 (원광대학교 광주한방병원 한방재활의학과) ;
  • 김수학 (원광대학교 광주한방병원 한방재활의학과) ;
  • 이학송 (학송한의원) ;
  • 염승룡 (원광대학교 광주한방병원 한방재활의학과) ;
  • 권영달 (원광대학교 광주한방병원 한방재활의학과)
  • Received : 2023.09.25
  • Accepted : 2023.10.14
  • Published : 2023.10.31
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Abstract

Objectives The anti-inflammatory and anti-arthritic effects of Youngseonjeatonguem (靈仙除痛飮, YSJTU) was evaluated in a cellular model using RAW264.7 macrophages, which are involved in osteoarthritis (OA), and an animal model using Sprague-Dawley (SD) rats, and a possible mechanism of anti-arthritic actions of YSJTU was presented. Methods RAW264.7 macrophages were activated by lipopolysaccharide (LPS). The production of pro-inflammatory cytokines (tumor necrosis factor [TNF]-𝛼, interleukin [IL]-1𝛽, and IL-6) and inflammatory mediators (nitric oxide [NO] and prostaglandin E2 [PGE2]) was determined by ELISA and Griess assay, respectively. Western blot was performed to determine inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. OA was induced by intra-articular injection of monosodium iodoacetate (MIA) into the right knee joint of SD rats. Results In RAW264.7 macrophages, YSJTU reduced LPS-induced production of TNF-𝛼, IL-1𝛽, and IL-6. In addition, YSJTU inhibited LPS-induced production of NO and PGE2 by suppressing iNOS and COX-2 expression. In SD rats, YSJTU improved MIA-induced OA by reducing swelling, skin heat, and cartilage degradation. In addition, YSJTU reduced serum levels of TNF-𝛼, IL-1𝛽, and IL-6, along with its significant decrease in serum levels of NO and PGE2. Conclusions These results suggest that YSJTU may exert anti-arthritic effect, at least in part, by inhibiting macrophage-mediated joint inflammation.

Keywords

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