Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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Complete Genome Analysis of Hyphantria cunea Nucleopolyhedrovirus Isolated in Korea

한국에서 분리한 미국흰불나방 핵다각체병 바이러스의 전장 유전체 분석

  • 최재방 ((주)옵티팜 기업부설연구소) ;
  • 김현수 (충북대학교 농업과학기술연구소) ;
  • 우수동 (충북대학교 식물의학과)
  • Received : 2023.08.31
  • Accepted : 2023.10.10
  • Published : 2023.11.30
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Abstract

The morphology and whole genome sequence of Hyphantria cunea nucleopolyhedrovirus W1 (HycuNPV-W1) isolated in Korea were analyzed for the use as an eco-friendly control agent against H. cunea. The HycuNPV-W1 had irregular tetrahedral polyhedra with a size of 1.5-2.2 ㎛ which is similar to that of previously reported HycuNPV isolated in Korea. As a result of whole viral genome analysis, HycuNPV-W1 was composed of 131,353 bp, which is 1,606 bp shorter than that of the previously reported HycuNPV. The G+C content was 45% and six of the homologous repeated regions were found, so there was no significant difference from the previous report. As a result of ORF analysis, HycuNPV-W1 contains total of 145 ORFs which is three ORFs less than the previous report, while two ORFs were exclusively found in HycuNPV-W1. The functions of these ORFs remains unclear and are not considered to have a significant influence on the characteristics of the HycuNPV. The genome vista analysis showed that the overall sequence identity between HycuNPV-W1 and the previously reported HycuNPV was very high. The whole genome of HycuNPV-W1 analyzed was found to be similar to those of the previously reported HycuNPV, however, it is supposed to be a novel resource in Korea with different isolate.

광식성 난방제 해충인 미국흰불나방(Hyphantria cunea)의 친환경 방제를 위한 바이러스 살충제의 소재 활용을 위해서 국내에서 분리된 미국흰불나방 핵다각체병바이러스(H. cunea nucleopolyhedrovirus W1: HycuNPV-W1)의 다각체 형태 및 전장 유전체 서열을 결정하고 분석하였다. HycuNPV-W1의 다각체는 1.5-2.2 um 크기의 사면체에 가까운 부정형으로 확인되었다. 전장 유전체의 염기서열을 결정한 결과, 기존에 보고된 HycuNPV와 비교할 때 1,606 bp 더 짧은 131,353 bp로 확인되었다. 그러나 G+C 함량은 45%였으며 상동반복영역은 6개로 기존에 보고된 HycuNPV와 차이는 확인할 수 없었다. ORF 분석에서는 HycuNPV-W1은 기존의 HycuNPV와 비교할 때 3개의 ORF가 더 적은 145개를 가졌으나, HycuNPV-W1에만 존재하는 2개의 ORF가 확인되었다. 이들 ORF의 기능은 현재까지 밝혀지지 않았으나 바이러스의 생물학적 특성에 큰 영향을 주지 않을 것으로 추정되었다. 유전체의 vista 분석 결과에서는 HycuNPV-W1과 기존 HycuNPV의 전체 염기서열 유사도가 매우 높은 것으로 확인되었다. 국내에서 처음으로 분석한 HycuNPV-W1의 전장 유전체는 기존의 HycuNPV와 매우 유사한 것으로 나타났으나, 독자적인 특성을 가진 서로 다른 분리주로 국내 고유자원임을 확인할 수 있었다.

Keywords

Acknowledgement

이 논문은 충북대학교 국립대학육성사업(2022) 지원을 받아 작성되었음.

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