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Selection of Entomopathogenic Fungi Against Bemisia tabaci and Culture Characterization on Spent Coffee Grounds Medium

담배가루이에 대한 곤충병원성 곰팡이 선발 및 커피박 배지에서의 배양 특성

  • 장지원 (전북대학교 농생물학과) ;
  • 허인지 (전북대학교 농생물학과) ;
  • 황동영 (전북대학교 농생물학과) ;
  • 김슬기 (전북대학교 농생물학과) ;
  • 신태영 (전북대학교 농생물학과)
  • Received : 2023.10.23
  • Accepted : 2023.11.10
  • Published : 2023.11.30

Abstract

The silverleaf whitefly, Bemisia tabaci, is a major pest distributing worldwide damaging over 900 host plant species, and is highly resistant to chemical pesti- cides. Due to the high pesticide resistance of whitefly, there is a need for alternatives to chemical control. Entomopathogenic fungi are candidates for biological pesticide that can overcome the resistance problem of chemical pesticide. Therefore, in this study, we tested pathogenicity of the entomopathogenic fungi to select high insec- ticidal activity against whitefly. As a result, IPBL-C (Cordyceps fumosorosea) and IPBL-F (Metarhizium pinghaense) isolates showed high insecticidal activity against whitefly. Additionally, as a result of culturing the selected isolates on spent coffee grounds medium, the conidia of IPBL-F produced on coffee grounds medium showed five times higher heat stability after heat treatment at 45℃ for one hour than conidia produced on PDA medium.

Keywords

Acknowledgement

본 결과물은 농림축산식품부의 재원으로 농림식품기술기획평가원의 농식품기술융합창의인재양성 사업의 지원을 받아 연구되었음(no. 321001-03).

References

  1. Ali, S., Z. Huang, and S. Ren. 2009. Media composition influences on growth, enzyme activity, and virulence of the entomopathogen hyphomycete Isaria fumosoroseus. Entomologia Experimentalis et Applicata. 131(1): 30-38.  https://doi.org/10.1111/j.1570-7458.2009.00833.x
  2. Boaventura, H. A., E. D. Quintela, E. N. Santos, J. F. A. Silva, and R. A. Humber. 2021. Susceptibility of all nymphal stages of Bemisia tabaci Biotype B (Hemiptera: Aleyrodidae) to three brazilian isolates of Cordyceps sp. (Hypocreales: Cordycipitaceae) in a screenhouse under variable temperature and moisture conditions. Neotropical Entomology. 50: 100-113.  https://doi.org/10.1007/s13744-020-00823-5
  3. Cafarchia, C., R. Pellegrino, V. Romano, M. Friuli, C. Demitri, M. Pombi, and D. Otranto. 2022. Delivery and effectiveness of entomopathogenic fungi for mosquito and tick control: Current knowledge and research challenges. Acta Tropica. 106627. 
  4. Campos-Vega, R., G. Loarca-Pina, H. A. Vergara-Castaneda, and B. D. Oomah. 2015. Spent coffee grounds: A review on current research and future prospects. Trends in Food Science & Technology. 45(1): 24-36.  https://doi.org/10.1016/j.tifs.2015.04.012
  5. Shin, M. and Y. H. Kim. 2018. Extraction of Caffeine from Spent Coffee Grounds and Oxidative Degradation of Caffeine. Journal of Environmental Science International. 27(12):1205-1214.  https://doi.org/10.5322/JESI.2018.27.12.1205
  6. Heo, I., S. Kim, G. H. Han, S. Im, J. W. Kim, D. Y. Hwang, and T. Y. Shin. 2023. Characteristics of insecticidal substances from the entomopathogenic fungus Metarhizium pinghaense 15R against cotton aphid in Korea. Journal of Asia-Pacific Entomology. 26(1):102013. 
  7. Kim, C. S., J. B. Lee, B. S. Kim, M. H. Lee, K. M. Kang, W. H. Joo, and G. S. Kwon. 2013. The optimal condition and enzyme activity of entomopathogenic fungus Beauveria bassiana using extracted rice bran. Journal of Life Science. 23(8): 1010-1018.  https://doi.org/10.5352/JLS.2013.23.8.1010
  8. Kim, J. C., M. R. Lee, S. Kim, S. J. Lee, S. E. Park, S. Baek, and J. S. Kim, 2019. Long-term storage stability of Beauveria bassiana ERL836 granules as fungal biopesticide. Journal of Asia-Pacific Entomology. 22(2): 537-542.  https://doi.org/10.1016/j.aspen.2019.04.001
  9. Kim, J. S., A. Kassa, M. Skinner, T. Hata, and B. L. Parker. 2011. Production of thermotolerant entomopathogenic fungal conidia on millet grain. Journal of Industrial Microbiology and Biotechnology. 38(6): 697-704.  https://doi.org/10.1007/s10295-010-0850-2
  10. Kim, M. S., N. Koo, and J. G. Kim. 2012. Characteristics and recycling of spent coffee grounds. Life Science and Natural Resources Research. 20: 59-69. 
  11. Kim, S., S. J. Kim, S. Cho, and S. H. Lee. 2021. Insecticide Resistance Monitoring of Bemisia tabaci (Hemiptera: Aleyrodidae) in Korea. Korean journal of applied entomology. 60(2): 167-173.  https://doi.org/10.5656/KSAE.2020.12.0.082
  12. Klingen, I., J. Eilenberg, and R. Eilenberg. 2002. Effects of farming system, field margins and bait insect on the occurrence of insect pathogenic fungi in soils. Agriculture, ecosystems & environment. 91(1-3): 191-198.  https://doi.org/10.1016/S0167-8809(01)00227-4
  13. Kwasniewska-Sip, P., G. Cofta, and P. B. Nowak. 2018. Resistance of fungal growth on Scots pine treated with caffeine. International Biodeterioration & Biodegradation. 132: 178-184.  https://doi.org/10.1016/j.ibiod.2018.03.007
  14. Kwon, T. H., C. G. Park, B. H. Lee, I. H. Jeong, and S. E. Lee. 2022. A New Approach: Ethyl Formate Fumigation to Control Bemisia tabaci (Hemiptera: Aleyrodidae) in a Yellow Melon Vinyl House. Applied Sciences. 12(10): 5173. 
  15. Lee, S. J., T. Y. Shin, J. C. Kim, and J. S. Kim. 2022. Entomopathogenic Fungi-mediated Pest Management and R&D Strategy. Korean journal of applied entomology. 61(1): 197-210.  https://doi.org/10.5656/KSAE.2022.02.1.065
  16. Li, Z., S. B. Alves, D. W. Roberts, M. Fan, I. Delalibera Jr, J. Tang, and D. E. Rangel. 2010. Biological control of insects in Brazil and China: history, current programs and reasons for their successes using entomopathogenic fungi. Biocontrol Science and Technology. 20(2):117-136.  https://doi.org/10.1080/09583150903431665
  17. Litwin, A., M. Nowak, and S. Rozalska. 2020. Entomopathogenic fungi: unconventional applications. Reviews in Environmental Science and Bio/Technolog. 19(1): 23-42.  https://doi.org/10.1007/s11157-020-09525-1
  18. Mascarin, G. M., N. N. Kobori, E. D. Quintela, and I. Delalibera Jr. 2013. The virulence of entomopathogenic fungi against Bemisia tabaci biotype B (Hemiptera: Aleyrodidae) and their conidial production using solid substrate fermentation. Biological control. 66(3): 209-218.  https://doi.org/10.1016/j.biocontrol.2013.05.001
  19. Park Y., H. Y. Nam, and J. Lee. 2019. Occurrence of Bemisia tabaci JpL (Hemiptera: Aleyrodidae) on Jeju Island in Korea. Korean journal of applied entomology. 58(3):203-7.  https://doi.org/10.5656/KSAE.2019.08.0.030
  20. Park, S. H., J. H. Lee, J. H. Woo, S. Y. Choi, S. D. Park, and H. H. Park. 2014. Economic Injury Level of Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae) on Oriental Melon (Cucumis melo L.) in Greenhouse. The Korean Journal of Pesticide Science. 18(3): 196-201.  https://doi.org/10.7585/kjps.2014.18.3.196
  21. Song, J. H., H. M Ahn, H. D. Jeung, S. O. Chung, and H. W. Kan. 2019. Growth of two mud shrimps (Upogebia major and Austinogebia wuhsienweni) settled in Boryeong and Hongseong tidal flat. Korean Journal of Environmental Biology. 37(2): 217-227.  https://doi.org/10.11626/KJEB.2019.37.2.217
  22. Taleb, F., M. Ammar, M. B. Mosbah, R. B. Salem, and Y. Moussaoui. 2020. Chemical modification of lignin derived from spent coffee grounds for methylene blue adsorption. Scientific Reports. 10(1): 11048. 
  23. Yoon, H. G., T. Y. Shin, M. R. Yu, W. W. Lee, S. H. Ko, S. M. Bae, and S. D. Woo. 2013. Characterization of entomopathogenic fungus from Trialeurodes vaporariorum and evaluation as insecticide. Korean Journal of Microbiology. 49(1): 64-70.  https://doi.org/10.7845/KJM.2013.006