Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Combined Effect of Afidopyropen, Chlorfenapyr and Cyantraniliprole to Insecticide-resistant Cotton Aphid, Aphis gossypii (Hemiptera: Aphididae)

살충제 저항성 목화진딧물에 대한 afidopyropen과 chlorfenapyr, cyantraniliprole의 혼합효과 평가

  • Dong-Hyun Kang (Department of Plant Medicine, Chungbuk National University) ;
  • Yuno Lee (Department of Plant Medicine, Chungbuk National University) ;
  • Ha Hyeon Moon (Department of Plant Medicine, Chungbuk National University) ;
  • Se Eun Kim (Department of Plant Medicine, Chungbuk National University) ;
  • Hyun-Na Koo (Department of Plant Medicine, Chungbuk National University) ;
  • Hyun Kyung Kim (Department of Plant Medicine, Chungbuk National University) ;
  • Gil-Hah Kim (Department of Plant Medicine, Chungbuk National University)
  • 강동현 (충북대학교 농업생명환경대학 식물의학과) ;
  • 이유노 (충북대학교 농업생명환경대학 식물의학과) ;
  • 문하현 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김세은 (충북대학교 농업생명환경대학 식물의학과) ;
  • 구현나 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김현경 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김길하 (충북대학교 농업생명환경대학 식물의학과)
  • Received : 2024.01.19
  • Accepted : 2024.02.19
  • Published : 2024.03.01
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Abstract

The susceptibility of Aphis gossypii populations collected from three fields (WJ, CC, and GS) was evaluated to three insecticides (afidopyropen, chlorfenapyr and cyantraniliprole) and three binary mixtures. Three field populations showed resistance ratios of over 100 to all insecticides. The Combination Index (CI), %M(synergism), Co-Toxicity Coefficient (CTC), Wadley Ratio (WR), Synergism Ratio (SR) and Abbott Ratio (AR) were used to evaluate combined effect of the insecticides. Afidopyropen + chlorfenapyr (CI ≤ 0.16; %M(synergism) ≥ 94; CTC ≥ 764.5; WR ≥ 6.4; SR ≥ 6.9 and AR ≥ 1.1) showed a synergism in all filed populations. WJ and CC populations showed a synergism in all binary mixtures of insecticides, but GS population showed an antagonism for chlorfenapyr + cyantraniliprole (CI, 1.63; %M(synergism), 30; CTC, 64.0; WR, 0.6 and AR, 0.54) and afidopyropen + cyantraniliprole (CI, 6.7; %M(synergism), 1; CTC, 19.8; WR, 0.2 and AR ≤ 0.55). All mixtures (afidopyropen + chlorfenapyr, chlorfenapyr + cyantraniliprole and afidopyropen + cyantraniliprole) showed a control value of over 99% after 21 days of treatment in the field. This study highlights that binary mixtures of three insecticides serve as an effective control strategy for A. gossypii.

살충제 저항성 목화진딧물 집단(완주, 춘천, 고성)에 대한 3종(afidopyropen, chlorfenapyr, cyantraniliprole) 약제별 감수성과 혼용에 따른 방제효과를 평가하였다. 3개 야외집단에 대해서 3약제 모두100배 이상의 저항성비를 보였다. 혼합제의 협력작용을 평가하기 위해 반수치사농도(LC50)을 이용한 조합지수(CI), %M(synergism), 연합독성계수(CTC), 와들리 비율(WR), 상승비율(SR)와 사충률을 이용한 아보트 비율(AR)로 비교하였다. Afidopyropen + chlorfenapyr은 모든 야외집단에 대해서 살충활성의 증가를 보였고, chlorfenapyr + cyantraniliprole, afidopyropen + cayntraniliprole은 완주, 춘천 집단에 대해서 살충활성의 증가를 보였다. 그러나 chlorfenapyr + cyantraniliprole (CI, 1.63; %M(synergism), 30; CTC, 64.0; WR, 0.6, AR, 0.54)과 afidopyropen + cyantraniliprole (CI, 6.7; %M(synergism), 1; CTC, 19.8; WR, 0.2, AR ≤ 0.55)은 고성 집단에서 길항작용을 나타내었다. 포장조건에서도 afidopyropen + chlorfenapyr, chlorfenapyr + cyantraniliprole, afidopyropen + cyantraniliprole은 99% 이상의 높은 방제가를 보여 향후 살충제 저항성 목화진딧물 방제용 혼합제 개발에 유용하게 활용될 것이다.

Keywords

Acknowledgement

본 논문은 농촌진흥청의 지원을 받아 연구되었습니다(RS-2022-RD010420).

References

  1. Abbott, W.S., 1925, A method of computing the effectiveness of an insecticide. J. Econ. Entomol. 18, 265-267.  https://doi.org/10.1093/jee/18.2.265a
  2. Attique, M.N., Khaliq, R.A., Sayyed, A.H., 2006. Could resistance to insecticides in Plutella xylostella (Lep., Plutellidae) be overcome by insecticide mixtures? J. Appl. Entomol. 130, 122-127.  https://doi.org/10.1111/j.1439-0418.2006.01035.x
  3. Bass, C., Nauen, R., 2023. The molecular mechanisms of insecticide resistance in aphid crop pests. Insect Biochem. Mol. Biol. 156, 103937. 
  4. Bernard, C., Philogene, B.J.R., 1993. Insecticide synergists: role, importance, and perspectives. J. Toxicol. Environ. 38, 199-223. 
  5. Blackman, R.L., Eastop, V.P., 2000. Aphids on the world's crops: An identification and information guide. Wiley & Sons, Chchester.
  6. Bliss, C.I., 1939. The toxicity of poisons applied jointly1. Ann. Appl. 26, 585-615.  https://doi.org/10.1111/j.1744-7348.1939.tb06990.x
  7. Brindley, W.A., Selim, A.A., 1984. Synergism and antagonism in the analysis of insecticide resistance. Environ. Entomol. 13, 348-354.  https://doi.org/10.1093/ee/13.2.348
  8. Byford, R.L., Lockwood, J.A., Sparks, T.C., 1987. A novel resistance management strategy for horn flies (Diptera: Muscidae). J. Econ. Entomol. 80, 291-296.  https://doi.org/10.1093/jee/80.2.291
  9. Carletto, J.E., Lombaert, P., Chavigny, T., Brevault, L., Vanlerberghe-Masutti, F., 2009. Ecological specialization of the aphid Aphis gossypii Glover on cultivated host plants. Mol. Ecol. 18, 2198-2212.  https://doi.org/10.1111/j.1365-294X.2009.04190.x
  10. Casida, J.E., 1970. Mixed-function oxidase involvement in the biochemistry of insecticide synergists. J. Agric. Food Chem. 18, 753-772.  https://doi.org/10.1021/jf60171a013
  11. Chen, X., Tie, M., Chen, A., Ma, K., Li, F., Liang, P., Liu, Y., Song, D., Gao, X., 2017, Pyrethroid resistance associated with M918L mutation and detoxifying metabolism in Aphis gossypii from cotton growing regions of China. J. Pest Manag. Sci. 73, 2353-2359.  https://doi.org/10.1002/ps.4622
  12. Chou, T.C., 2010. Drug combination studies and their synergy quantification using the Chou-Talalay Method. Cancer Res. 70, 440-446.  https://doi.org/10.1158/0008-5472.CAN-09-1947
  13. Colby, S.R., 1967. Calculating synergistic and antagonistic responses of herbicide combinations. Weeds World: The International Electronic Arabidopsis Newsletter 15, 20-22. 
  14. Ebert, T.A., Cartwright, B., 1997. Biology and ecology of Aphis gossypii Glover (Homoptera: aphididae). Southwest. Entomol. 22, 116-153. 
  15. European and Mediterranean Plant Protection Organization (EPPO), 2018. PP 1/306 (1) General principles for the development of co-formulated mixtures of plant protection products 42, 353-357. https://doi.org/10.1111/epp.2608 
  16. Finney, D., 1942. The analysis of toxicity tests on mixtures of poisons. Ann. Appl. 29, 82-94.  https://doi.org/10.1111/j.1744-7348.1942.tb06923.x
  17. Foster, S.P., Denholm, I., Rison, J.L., Portillo, H.E., Margaritopoulis, J., Slater, R., 2012. Susceptibility of standard clones and european field populations of the green peach aphid, Myzus persicae, and the cotton aphid, Aphis gossypii (Hemiptera: Aphididae), to the novel anthranilic diamide insecticide cyantraniliprole. Pest Manag. Sci. 68, 629-633. 
  18. Freeman, J.C., Smith, L.B., Silva, J.J., Fan, Y., Sun, H., Scott, J.G., 2021. Fitness studies of insecticide resistant strains: lessons learned and future directions. Pest Manag. Sci. 77, 3847-3856.  https://doi.org/10.1002/ps.6306
  19. Georghiou, G.P., 2005. Principles of insecticide resistance management. Phytoprotection 75, 51-59.  https://doi.org/10.7202/706071ar
  20. Georghiou, G.P., Taylor, C.E., 1986. Factors influencing the evolution of resistance in: National Research Council (Ed.), Pesticide resistance: strategies and tactics for management. National Academy Press, Washington, D.C, pp. 157-169. 
  21. Gleave, K., Lissenden, N., Chaplin, M., Choi, L., Ranson, H., 2021. Piperonyl butoxide (PBO) combined with pyrethroids in insecticide-treated nets to prevent malaria in Africa. Corhrane Database Syst. Rev. 2021, 5CD012776. 
  22. Iverson, A., Hale, C., Richardson, L., Miller, O., McArt, S., 2019. Synergistic effects of three sterol biosynthesis inhibiting fungicides on the toxicity of a pyrethroid and neonicotinoid insecticide to bumble bees. Apidologie 50, 733-744.  https://doi.org/10.1007/s13592-019-00681-0
  23. Kang, D.H., Lee, U.N., Kim, S.E., Moon, H.H., Kim, S.Y., Jung, J.W., Koo, H.N., Kim, H.K., Kim, G.H., 2023. Susceptibility assessment of cotton aphid, Aphis gossypii (Hemiptera: Aphidiae) field populations in Korea to 11 insecticides. Korean J. pestic. Sci. 27, 259-271. (In Korean)  https://doi.org/10.7585/kjps.2023.27.3.259
  24. Khan, H.A.A., Akram, W., Shad, S.A., Lee, J.J., 2013. Insecticide mixtures could enhance the toxicity of insecticides in a resistant dairy population of Musca domestica L. PloS One 8, e60929. https://doi.org/10.1371/journal.pone.0060929 
  25. Kim, G.H., Cho, K.Y., Choi, S.Y., 1987. Joint toxic action of pyrethroids and primicarb mixtures to green peach aphid (Myzus persicae Sulzer). J. Appl. Entomol. 17, 179-183. 
  26. Koo, H.N., An, J.J., Park, S.E., Kim, J.I., Kim, G.H., 2014. Regional susceptibilities to 12 insecticides of melon and cotton aphid, Aphis gossypii (Hemiptera: Aphididae) and a point mutation associated with imidacloprid resistance. Crop Prot. 55, 91-97.  https://doi.org/10.1016/j.cropro.2013.09.010
  27. Lee, J.M., Jeon, J.C., Kang, W.J., Kim, H.K., Park, B., Koo, H.N., Kim, G.H., 2022. Analysis of point mutations associated with fenvalerate and imidacloprid resistant cotton aphids, Aphis gossypii (Hemiptera: Aphididae) and selection of insecticides for effective control. Korean J. pestic. Sci. 26, 140-49. (In Korean)  https://doi.org/10.7585/kjps.2022.26.2.140
  28. Levy, Y., Benderly, M., Cohen, Y., Gisi, U., Bassand, D., 1986. The joint action of fungicides in mixtures: comparison of two methods for synergy calculation. Bull. OEPP. 16, 651-657.  https://doi.org/10.1111/j.1365-2338.1986.tb00338.x
  29. Li, R., Cheng, S., Liang, P., Chen, Z., Zhang, Y., Liang, P., Zhang, L., Gao, X., 2022. Status of resistance of Aphis gossypii Glover, 1877(Hemiptera: Aphididae) to afidopyropen originating from microbial secondary metabolites in China. Toxin 13, 1157. 
  30. Loewe, S., 1928. Die quantitativen probleme der pharmakologie. Ergeb Physiol. 27, 147-187.  https://doi.org/10.1007/BF02322290
  31. Madgwick, P.G., Kanitz, R., 2023. Beyond redundant kill: a fundamental explanation of how insecticide mixtures work for resistance management. Pest Manag. Sci. 79, 495-506.  https://doi.org/10.1002/ps.7180
  32. Martin, T., Ochou, G.O., Maurice, V., Didier, F., 2003. Organophosphorus insecticides synergize pyrethroids in the resistant strain of cotton bollworm, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) from West Africa. J. Econ. Entomol. 96, 468-474.  https://doi.org/10.1603/0022-0493-96.2.468
  33. Ministry of Agriculture and Forestry, 2000. Development of selective proinsecticide and low-input pesticide mixtures. 
  34. Nam, H.Y., Kim, S., Lee, S.H., Lee, J.H., Kim, J.I., 2022. Insecticide resistance in pepper greenhouse populations of Aphis gossypii (Hemiptera: Aphididae) in Korea. Pestic. Biochem. Physiol. 182, 105033. 
  35. Nauen, R., Elbert, A., 2003. European monitoring of resistance to insecticides in Myzus persicae and Aphis gossypii (Hemiptera: Aphididae) with special reference to imidacloprid. Bull. Entomol. Res. 93, 47-54.  https://doi.org/10.1079/BER2002215
  36. Roy, D., Biswas, S., Biswas, A., Chakraborty, G., Sarkar, P.K., 2022. Can insecticide mixtures be considered to surmount neonicotinoid resistance in Bemisia tabaci? J. Asia Pac. Entomol. 25, 101901. 
  37. SAS Institute, 2019. SAS certified professional prep guide: advanced programming using SAS 9.4. SAS Institute. 
  38. Somar, O.R., Zamani, A.A., Alizadeh, M., 2019. Joint action toxicity of imidacloprid and pymetrozine on the melon aphid, Aphis gossypii. Crop Prot. 124, 104850. 
  39. Son, S.H., 2004. Development of amitraz and pyridaben mixture for the control of acaricide-resistant two-spotted spider mite, Tetranychus urticae (Acarina: Teranichidae). Master's Thesis, Chungbuk National University, Cheongju. 
  40. Sparks, T.C., Lorsbach, B.A., 2017. Agrochemical discovery - building the next generation of insect control agents in: Gross, A.D., Ozoe, Y., Coats, J.R., (Eds.), Advances in agrochemicals: ion channels and G protein-coupled receptors (GPCRs) as targets for pest control, ACS Symposium Series Vol. 1264, ACS Publications, Washington, DC, pp. 1-17. 
  41. Sun, Y.P., Johnson, E.R., 1960. Analysis of joint action of insecticides against house flies. J. Econ. Entomol. 53, 887-892.  https://doi.org/10.1093/jee/53.5.887
  42. Taillebois, E., Thany, S.H., 2022. The use of insecticide mixtures containing neonicotinoids as a strategy to limit insect pests: efficiency and mode of action. Pestic. Biochem. Physiol. 184, 105126. 
  43. U.S. Environmental Protection Agency, 1986. Guidelines for the health risk assessment of chemical mixtures. U.S Environmental Protection Agency Washinton, DC, 51, 34014-34025. 
  44. Wadley, F.M., 1945. The evidence required to show synergistic action of insecticides and a short cut in analysis. US Department of Agriculture, Bureau of Entomology and Plant Quarantine. 
  45. Yang, Y.Y., Lai, C.T., 2019. Synergistic effect and field control efficacy of the binary mixture of permethrin and chlorpyrifos to brown planthopper (Nilaparvata lugens). J. Asia Pac. Entomol. 22, 67-76. https://doi.org/10.1016/j.aspen.2018.11.004 
  46. Zhang, J., Jiang, J., Wang, K., Zhang, X., Liu, Z., Yu, N., 2022. A binary mixture of emamectin benzoate and chlorantraniliprole supplemented with an adjuvant effectively controls Spodoptera frugiperda. Insects 13, 1157.