한국수정란이식학회:학술대회논문집 (Proceedings of the Korean Society of Embryo Transfer Conference) (Proceedings of the Korean Society of Embryo Transfer Conference)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
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한국수정란이식학회 2004년도 제4회 발생공학 국제심포지움 및 학술대회
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This work was undertaken in order to study the developmental competence of nuclear transfer cat embryo with fetal fibroblast and adult skin fibroblast as donor nuclei. Oocytes wererecovered by mincing the ovaries in Hepes-buffered TCM199 and selected the cumulus oocyte complexes (COCs) with compact cumulus cell mass and dark. Homogenous ooplasm were cultured for maturation in TCM199 + 10% fetal bovine serum (FBS) for 12 hours and used as a source of recipient cytoplast for exogenous somatic nuclei. In Experiment 1, we evaluated the effect donor cell types on the reconstruction and development of cloned embryos. Fusion, first cleavage and blastocyst developmental rate was not different between fetal fibroblast and adult skin cell (71.2 vs. 66.8; 71.0 vs. 57.6; 4.0 vs. 6.1 %, P<0.05). In Experiment 2, cloned embryos were surgically transferred into the oviducts of recipient queens. One of seven recipient queens was delivered naturally 2healthy cloned cats and 1 stillborn from fetal fibroblast cell of male origin after 65 days embryo transfer. One of three recipient queens was delivered naturally 1 healthy cloned cat from adult skin cell of female after 65 days embryo transfer. The cloned cats showed genotypes identical to the donor cell lines, indicating that adult somatic cells can be used for feline cloning.
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Hyun, Sang-Hwan;Simerly, Calvin;Navara, Christopher;Kang, Sung-Keun;Lee, Byeong-Chun;Schatten, Gerald;Hwang, Woo-Suk 32
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Kang, Il-Hyun;Shin, Jae-Ho;Moon, Hyun-Ju;Kim, Tae-Sung;Ki, Ho-Youn;Kim, Yun-Sook;Dong, Mi-Sook;Han, Soon-Young;Kang, Tae-Seok 42
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Kim, Min-Kyu;Jang, Goo;Oh, Hyun-Ju;Fibrianto, Yuda Heru;Kim, Hye-Jin;Hossein, M. Shamim;Kang, Sung-Keun;Lee, Byeong-Chun;Hwang, Woo-Suk 49
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Kim, Jung-Gon;Jin, Hai-Feng;Song, Hye-Jin;Shi, Lian-Yu;Kumar, Basavarajappa Mohana;Ock, Sun-A;Rho, Gyu-Jin;Choe, Sang-Yong 55
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Kim, Ju-Ran;Yu, Seong-Jin;Kang, Hee-Gyoung;Do, Byung-Rok;Koh, Seong-Ho;Kim, Kyung-Suk;Kim, Seung-Hyun;Park, Se-Ah;Kim, Hae-Kwan;Kang, Sung-Goo 57
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Kim, Jee-Yun;Kim, Ju-Ran;Yu, Seong-Jin;Kang, Ji-Hye;Kang, Hee-Gyoung;Lee, Chae-Kwan;Kang, Sung-Goo 59
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Kim, Hong-Rae;Kang, Jae-Ku;Yoon, Jong-Taek;Seong, Hwan-Hoo;Jo, Min-Rae;Park, Chang-Sik;Jin, Dong-Il 62
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Hong, Seok-Ho;Nah, Hee-Young;Lee, Young-Jin;Lee, Ji-Won;Kim, Chung-Hoon;Kim, Sung-Hoon;Chae, Hee-Dong;Kang, Byung-Moon;Kim, Moon-Kyoo 65
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Park, Ji-Hyun;Choi, Tae-Won;Kim, Bong-Woo;Kim, Jung-Hyun;Oh, Young-Jun;Choi, Eui-Ju;Cho, Ssang-Goo 73
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Oh, Hyun-Ju;Kim, Min-Kyu;Fibrianto, Yuda Heru;Jang, Goo;Kim, Hye-Jin;Hossein, M. Shamim;Kang, Sung-Keun;Lee, Byeong-Chun;Hwang, Woo-Suk 83
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Yu, Seong-Jin;Kim, Ju-Ran;Kim, Jee-Yun;Kang, Hee-Gyoung;Kang, Ji-Hye;Lee, Chae-Kwan;Kang, Sung-Goo 85
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Lee, Gun-Soup;Lee, Do-Hun;Kim, Eun-Young;Wang, Kyu-Chang;Lee, Won-Don;Park, Se-Pill;Lim, Jin-Ho 88
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Lee, So-Hyun;Kim, Sue;Jeong, Yeon-Woo;Kim, Ji-Hye;Koo, Ok-Jae;Lee, Eu-Gene;Park, Seon-Mi;Hashem, MD abul;Hossein, Mohammad shamim;Kang, Sung-Keun;Lee, Byeong-Chun;Hwang, Woo-Suk 91
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Lee, Chang-Hyun;Cho, Hwang-Yun;Kim, Yong-Sik;Kim, Eun-Young;Lee, Won-Don;Park, Se-Pill;Lim, Jin-Ho 98
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Jeong, Hyeong-Bok;Oh, Dae-Ju;Park, Ji-Gweon;Hyun, Kyung-Man;Kang, Sung-Il;Han, Jae-Woong;Hong, Jae-Young;Choi, Seong-Jin;Kim, Se-Jae 104
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Ju, Eun-Jin;Kwak, Dong-Hoon;Lee, Dae-Hoon;Kim, Sung-Min;Jung, Kyu-Yong;Lee, Seo-Ul;Do, Su-Il;Park, Yong-Il;Choo, Young-Kug 106
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Choi, Seung-Kyu;Jung, Joo-Young;Hong, Su;Lee, Hwang;Choi, Sung-Sik;Park, Chang-Sik;Lee, Sang-Ho 111
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Han, Ji-Young;Park, Se-Ah;An, Su-Yeon;Kim, Kyung-Suk;Kim, Seung-H;Koh, Seung-Ho;Roh, Won-Jae;Do, Byung-Rok;Yoon, Yong-Dal;Kim, Haek-Won 123