• Korean Journal of Ecology and Environment
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• v.48 no.4
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• pp.263-271
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• 2015

To evaluate the relationship between dynamics of cyanobacteria and odorous compounds, a monthly monitoring of water quality and phytoplankton were conducted at the three serial lakes (Lake Ui-am, Lake Cheong-pyeong and Lake Pal-dang) in the North Han River for 11 times from May 2014 to March 2015. In the three serial lakes, phytoplankton communities showed that seasonal changes in Bacillariophyceae-Cyanophyceae-Bacillariophyceae. Anabaena and Pseudanabaena were dominant species in August and September 2014. At the same time the odors (Geosmin, 2-MIB) were also detected with high concentration. Relationship between environmental factors and cyanobacterial abundance showed a significant correlation with Anabaena circinalis and geosmin (r=0.983, p<0.01). In the case of Pseudanabaena limnetica showed a significant correlation of total nitrogen (r=0.685, p<0.01) and NO3-N (r=0.723, p<0.01). In addition, similarly Pseudanabaena limnetica and 2-MIB (r=0.717, p<0.01) was high. The odorous compounds appeared in the North Han River water were considered to be a direct relationship with cyanobacteria.

• Korean Journal of Ecology and Environment
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• v.42 no.3
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• pp.364-373
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• 2009

We evaluated stream health, based on integrative approaches of qualitative habitat evaluation index (QHEI), chemical water quality and the index of biological integrity (IBI) using fish assemblages in Daejeon Stream during August 2004 - October 2007. The QHEI values in this study averaged 78.2 (n=28, range: 47$\sim$124), indicating "fair" condition based on the criteria of US EPA (1993). The highest value was in the headwater site (S1) that was 112 (good), whereas the value in S3, mid and downstream was SS (fair-poor) that was the lowest owing to a disturbance on the bed and an artificial bank. Concentrations of chemical parameters such as BOD, TP and conductivity increased from the upstream to the downstream due to higher inputs of organic matters and nutrients from the urban area. Conductivity and TP showed a dilution of stream water by rainwater during summer monsoon. Analysis of fish compositions showed that total number of species was 24 and Zacco platypus was predominated (82%). The mean IBI was 17 (range: 12$\sim$30) during four years and the IBI values also decreased from S1 to S4. These results indicated that ecological health was degradated distinctly as the stream goes down through the city-center. We concluded that Daejeon Stream running through the city are influenced by chemical impact such as an influx of domestic sewage as well as the quality of habitat.

• Korean Journal of Ecology and Environment
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• v.51 no.3
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• pp.221-233
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• 2018

This study was carried out to predict the current and future potential distribution and to identify the factors affecting potential distribution of 7 plants(Lamium amplexicaule L., Trigonotis peduncularis(Trevir.) Benth. ex Hemsl, Capsella bursa-pastoris (L.) L. W. Medicus, Taraxacum officinale Weber, Veronica persica Poir., Conyza sumatrensis E. Walker, Hypochaeris radicata L.) selected as indicators for climate change in agricultural ecosystem. We collected presence/absence data of 7 indicator plants at 108 sites in South Korea and applied the Maxent model. According to future climate scenario, the distribution area of C. bursa-pastoris(L.) L. W. Medicus, T. officinale Weber, and V. persica Poir. was expected to be reduced, but the distribution range was to be maintained. The distribution areas and range of the C. sumatrensis E. Walker and H. radicata L. were expected to be increased. The distribution area and range of T. peduncularis (Trevir.) Benth. Ex Hemsl. and L. amplexicalue L. were rapidly decreased. Non-climatic factors such as land cover and altitude were the most important environmental variable for T. officinale Weber, C. bursa-pastoris(L.) L.W.Medicus, V. persica Poir., T. peduncularis (Trevir.) Benth. Ex Hemsl., and L. amplexicalue L.. Climatic factors were the most important environmental variable for C. sumatrensis E. Walker and H. radicata L.. It is expected that the future potential distribution of 7 indicator plants response to climate change will be used to monitor and to establish the management plan.

• Korean Journal of Ecology and Environment
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• v.53 no.1
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• pp.63-72
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• 2020

In this study, to discuss on the applicability of eDNA as a new method to investigate fish diversity at streams, we applied eDNA at 4 streams (Geum River, Ji Stream, Hwangji Stream, Seomjin River), where endangered species are inhabits, with conventional survey (cast net and kick net). The average (±standard deviation) number of species investigated by eDNA were 19 species (±4.4), and it was relatively higher than average of conventional survey, 10 species (±4.8). Most of case, in this study, eDNA was more efficient than conventional survey. However, there were errors on species identification of Korean endemic species and aliied species from eDNA, and it seems the universal primer (MiFish primer set) is not suitable for them. Furthermore, some of endangered species, caught by conventional method, was not detected by eDNA. As the present universal primer is not suitable for identify the every freshwater fish species in Korea, the complementing or development of universal primer is needed, and the eDNA application after species specific marker development for detecting specific species like endangered species should be considered. In conclusion, if the manual for field survey method by eDNA is developed, we expect applicability enlargement for water ecosystem survey.

• Journal of Life Science
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• v.16 no.5
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• pp.729-733
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• 2006

Cryptosporidium is a waterborne pathogenic parasite which causes diarrhea. Immunomagnetic separation-immunofluorescent assay (IMS-IFA) has been a widely adopted for Cryptosporidium detection as standard method. However, this method does not provide information about viability or infectivity of Cryptosporidium. Therefore, many researchers have studied viability or infectivity analyses of Cryptosporidium with various methods such as vital staining, in vitro excystation, RT-PCR, cell culture, and mouse infection assay. In this study, two direct RT-PCR methods, cell culture RT-PCR and cell culture IFA were compared for sensitivity and other characteristics. The results showed that direct RT-PCR method with HSP70 genes had the highest sensitivity with detection up to 1 viable cell of Cryptosporidium. The infectious Cryptosporidium were detected up to 10 to 25 cells by cell culture methods in combination with RT-PCR and IFA. The infectious Cryptosporidium were apt to be quantified by cell culture IFA.

• Korean Journal of Clinical Laboratory Science
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• v.49 no.4
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• pp.382-389
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• 2017

Currently, reticulocyte experimental calculation technology used in clinical laboratories are divided two types: manual and automated. Manual reticulocyte counting using a microscopy lacks accuracy due particularly to its low reproducibility, affecting the accuracy of manual reticulocyte count. Moreover, Automatic blood corpuscle analyzer flow cytometry is difficult to be used in underdeveloped countries and small scale laboratories due to relatively high cost. Therefore, this study tried to find a new method to complement these drawbacks. The aim of this study was to compare the stained reticulocytes count by spectrophotometer and also to analyze the statistics of spectrophotometer and flow cytometer. The same 8 EDTA samples were repeated 36 times to compare the agreement between spectrophotometer and flow cytometer. This study measured the specimen diluted 600 times at 700~780 nm by 10 differences. Wavelength between 710 to 730 by absorbance showed a positive correlation between standard data and test data (r=0.967, p<0.01), presenting a correlation between variables. Statistical analyses of regression for test and standard parametric data, the optimal dilution factor was 600 times. Therefore, this study tried to technical utilizes such as contributing economical for the reticulocyte absorbance apply from the auto spectrophotometer, a monitoring system for the reticulocyte relation anemia, etc. Therefore, more extensive studies, including an auto chemical analyzer application, will be needed.

• Korean Journal of Clinical Laboratory Science
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• v.49 no.4
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• pp.427-434
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• 2017

Species that belong to the Acinetobacter calcoaceticus-baumannii (Acb) complex are major causes of hospital-acquired infections. They are important opportunistic pathogens. These species are usually multidrug resistant (MDR), and the therapeutic options to treat the infections caused by these species are limited. In the present study, we investigated fluoroquinolone resistance mechanisms in 53 ciprofloxacin resistant Acinetobacter species isolates in Chungcheong, Korea. Antimicrobial susceptibilities were determined using the disk-diffusion method. Detections of genes and identification of mutations associated with fluoroquinolone resistance were carried out using PCR and DNA sequencing. In our study, 47 out of 53 ciprofloxacin resistant Acinetobacter isolates harbored sense mutations at the 83rd residue (serine to leucine) in the gyrA gene as well as at the 80th residue (serine to leucine) in the parC gene. Among the 47 isolates harboring sense mutations in gyrA and parC gene, 44 isolates were A. baumannii and 3 isolates were A. pittii. Plasmid-mediated quinolone resistance (PMQR) determinants were detected in isolates in our study. Among the 46 ciprofloxacin resistant A. baumannii isolates, 41 showed type A, B, or F banding patterns on their REP-PCR profiles. This result suggests that clonal relation and horizontal spreading of the bacterial isolates have been around hospitals in Chungcheong area. To prevent colonization and disseminations of fluoroquinolone resistance Acb complex isolates, continuous investigation and monitoring of antimicrobial resistant determinants of MDR isolates are needed.

• Journal of Food Hygiene and Safety
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• v.24 no.1
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• pp.102-109
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• 2009

This study was carried out to analyse the microbio\logical contamination and biogenic amines(BA) content in Korea traditional soybean paste and commercial soybean paste. The results of microbio\logical analysis through Korean traditional soybean pastes($L1{\sim}L4$) were $7.8{\pm}0.1\;\log\;CFU/g{\sim}7.9{\pm}0.1\;\log\;CFU/g$, commercial soybean pastes($H1{\sim}H6$) were $6.2{\pm}0.1\;\log\;CFU/g{\sim}7.4{\pm}0.1\;\log\;CFU/g$ for APC (Aerobic Plate Count), and $L1{\sim}L4$, H5, H6 soybean pastes were $2.3{\pm}0.4\;\log\;CFU/g{\sim}2.6{\pm}0.1\;\log\;CFU/g$ for Bacillus cereus. But other microorganism was not dectected. Among biogenic amines, PUT(putrescine), TYR(tyramine), HIS(histamine), PHE(2-Phenylethylamine) were dectected high level and CAD(cadaverine), TRY(trypramine), AGM(agmatine) were dectected medium level and SPD(spermidine), SPM(spermine), NOR(noradrenaline), SER(serotonin) were dectected low level. Dectected contents of biogenic amines were higher in commercial soybean paste compared to the traditional soybean paste.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2006.05a
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• pp.312-315
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• 2006

축산물을 판매하고 있는 인터넷 및 통신판매업소에서 전자상거래 시 표지하여야 할 항목과 안전하고 위생적인 축산물의 확보를 위하여 표지하여야 할 항목으로 나누어 실태조사를 실시하였다. 전자상거래시 온라인(인터넷) 판매영업소가 표시하도록 규정하고 있는 항목 중에서 '상호명'과 '전화번호'를 제외한 나머지 정보는 일부 제품에서 누락되어 있는 것으로 나타났다. 특히 '배송비용 정보'(21% 미표시)와 '사이트 이용 약관'(23% 미표시)과 같은 정보는 상대적으로 높은 부적합률을 나타냈고, '이메일 주소'(22% 미표시)와 '팩스번호'(26% 미표시)의 부적합률은 가장 높게 조사되었다. 또한, 온라인 판매업소가 축산물을 판매할 때 표시하도록 규정되어 있는 항목 중에서 '제품명'과 '식육의 종류' 표시를 제외한 나머지 정보는 일부 제품에서 누락되어 있는 것으로 조사되었다. '식육의 부위'(19% 미표시)와 '제품의 구성'(27% 미표시)과 같은 정보는 상대적으로 높은 부적합률을 나타내었고, '보관방법'(57% 미표시), '품질보증 및 인증내용'(80% 미표시), '식육의 등급'(82% 미표시)과 같은 정보는 많은 제품에서 누락되어 있었으며, 특히 '제조일자'(95% 미표시)는 대부분의 제품에서 표기되어 있지 않은 것으로 조사되었다. 인터넷이나 통신판매업소를 통한 온라인 상거래에서는 육안으로 상품을 직접 확인할 수 없기 때문에 축산물의 위생 상태나 안전성을 파악하기 어려워 보다 확실하고 투명한 방법으로 축산물의 안전성과 위생 상태를 확인하는 수단으로 표시 규정을 100% 준수하여 품질의 고급화와 브랜드화를 이룩해 나가야 할 것으로 사료된다.타내었다.로 나타났다. 특히 골반골 현수는 출하 월령이 증가됨에 따라 관능 특성의 개선율이 높은 것으로 나타났다. 수 있다.분별을 성공적으로 수행하였다.(p<0.05), 맛, 연도, 다즙성 및 전체적인 기호성은 유의한 차이가 없었다.자체를 악하다고 볼 수 없고 더구나 구원을 이 세상에서의 이탈로 볼 수 없다. 진정한 구원이란 원래 하나님이 보시기에 아름다웠던 그 세상으로의 회복을 포함한다. 이런 면에서 하나님 주권 신앙 하에서 구원이란 전 인격적인 구원, 전 우주적인 구원이 된다. 그렇기 때문에 성도는 세상의 삶과 학문, 예술, 정치, 경제, 사회를 포함한 모든 분야를 하나님의 뜻 가운데서 그 원래의 목적에 부합할 수 있도록 회복시키는 일에 적극 참여해야 한다.자체가 이를 주도하기는 사실 어려움이 있다. 그리고 대형유통점이 영업행위를 영업시간제한에서부터 출점제한에 이르기까지 규제하는 건은 심사숙고하여야 한다. 대형유통점이 국가경제 및 지역사회에 미치는 영향이 부정적인가 긍정적인가에 대해 국내외 학계와 업계에서 여전히 많은 논란이 있기 때문이다. 정부와 지자체에 의한 시장개입은 반드시 필요한 경우에 한해 합당한 방법에 의해 이루어져야 한다. 대형유통점에 대한 규제는 지역사회에 미치는 영향을 다면적으로 평가한 결과에 근거하여 이루어져야 할 것이다. 대부분의 지자체는 체계적인 평가시스템과 객관적인 통계 자료를 갖고 있지 못한 실정이다. 향후 가장 시급한 과제는 시장개방 이후 지난 10년간 대형유통점이 지역사회에 미친 영향에 관한 광범위한 통계자료를 수집하고 이를 체계적으로 분석하여 정책방향을 올바르게 설정하는 것이라 할 수 있다.i와 K. pne

• Research in Plant Disease
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• v.11 no.2
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• pp.185-192
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• 2005

In 1999 and 2002, 130 and 258 isolates of Colletotrichum spp. causing red pepper anthracnose were obtained from infected red pepper fruits, respectively. Their responses to 4 protective and 3 ergosterol biosynthesis-inhibiting(EBI) fungicides were investigated by observing their mycelial growth on PDA incorporated with different concentrations of each fungicide. The Colletotrichum isolates obtained in 1999 showed higher $EC_{50}$ values than those isolated in 2002 against three protective fungicides such as dithianon, chlorothalonil, and propineb, whereas the response was reversed toward other protective fungicide, iminoctadine. On the other hand, the isolates of year 1999 were more resistant against three EBI fungicides such as tebuconazole, hexaconazole, and prochloraz than those of year 2002; the $EC_{50}$ values of the former were 1.2-4.4 times higher than those of the latter, The responses of the Colletotrichum isolates toward protective and EBI fungicides were fluctuated according to regions, where the infected fruits were collected. On the other hand, the resis tance of Colletotrichum isolates to protective fungicides increased during monitoring from July to September, However, their responses towards EBI fungicides were not changed.