• 제목/요약/키워드: Lactobacillus cellobiosus

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Characterization of Lactobacillus cellobiosus D37 Isolated from Soybean Paste as a Probiotic with Anti-Cancer and Antimicrobial Properties

  • Lim, Sung-Mee;Lee, Goon-Ja;Park, Sun-Mee;Ahn, Dong-Hyun;Im, Dong-Soon
    • Food Science and Biotechnology
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    • 제15권5호
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    • pp.792-798
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    • 2006
  • The probiotic characteristics of a total of 137 lactic acid bacterial strains isolated from soybean paste were investigated. Among those tested, the D37 strain was selected as a probiotic bacteria due to its acid and bile tolerance, and its strong anti-cancer and antibacterial activities. The D37 strain showed highly stable viability at acidic pH for 2 hr, and was very stable in 10% bovine bile. The viability of human colon cancer HT-29 cells was inhibited more than 60% at a $200\;{\mu}/mL$ concentration of D37 cell-free culture supernatant, and the degree of inhibition was concentration-dependent. The D37 strain showed a wide range of antibacterial activities against food-borne pathogenic bacteria such as Escherichia coli O157, Listeria spp., Vibrio spp., Salmonella spp., and Staphylococcus aureus. According to phenotypic characteristics and the utilization of various sugars, the D37 strain was identified as Lactobacillus cellobiosus.

한국여성의 질내 Lactobacillus 균종분포 (Analysis of Lactobacillus Species Isolated from the Vagina of Korean Women)

  • 김각균;국윤호;장우현
    • 대한미생물학회지
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    • 제16권1호
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    • pp.35-38
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    • 1981
  • Lactobacillus species isolated from posterior fornix of the vagina of 410 Korean women, who visited several local clinics and the Family Health Clinic of the Institute of Reproductive Medicine and Population, Seoul National University in Seoul were identified by their physiological and biochemical characteristics. And the results were as follows. 1) Of the 410 women 297(72.4%) gave the positive culture for Lactobacillus. 2) Isolated 200 strains were differentiated into following species. L. salivarius(43 strains, 18.7%) and L. leichmannii(42, 18.3%) were the most common species followed by L. plantarum(34, 14.8%) L.brevis(22, 9.6%), L. fermenti(19, 8.3%), L. cellobiosus(15, 6.5%), L. acidophilus(14, 6.1%), L. delbrueckii(10, 4.3%) and L. casei(1, 0.4%) and there were 30 strains(13%) which could not have been identified by the methods employed in this study. 3) Age seems to have no influence on the distribution of Lactobacillus species in vagina and it seems like that the overall distribution of Lactobacillus species in vagina does not change during the active reproductive period of women.

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유산균(乳酸菌)의 생물학적특성(生物學的特性)에 관한 연구(硏究) II. 약제내성(藥劑耐性) 유산균(乳酸菌)의 R Plasmids 전달빈도(傳達頻度) (Studies on Biological Characteristics of Lactobacillus II. Conjugal Transfer-frequency of R Plasmids from Lactobacillus to Escherichia coli)

  • 김종면;송희종
    • 대한수의학회지
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    • 제20권2호
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    • pp.113-118
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    • 1980
  • Total of 11 strains of Ldctobacillus isolated from lactobacillus-fermented milk and-beverage in March 1980 were examined for susceptibility to 8 drugs, and transferability and transfer frequency of R plasmids by conjugation. Of 11 isolates each 2 strains were classified as L. cellobiosus and L. helveticus, each 1 strain as L. plantarum, L. lactis, L. acidophilus, L. delbrueckii, L. casei subsp. casei, L. casei subsp, tolerans and L. salivarius subsp, salivarius by Bergey's manual. Resistance was the most active to na lidixic acid(NA), followed in decreasing order by chloramphenicol(CP), ampicillin(AP), kanamycin(KM) and streptomycin(SM). All of isolates were resistant to NA, each 10 strains to CP and AP, 7strains to KM and 6 strains to SM, indicating all of the isolates were resistant to two or more drugs in combination. No strain was resistant to erythromycin(EM), penicillin(PC) and tetracycline(TC). The most frequently encountered resistant patterns were CP NA AP SM KM, followed by CP NA AP KM, NA AP, CP NA, CP NA AP and CP NA AP SM in order. Transfer experiment of drug resistance showed that of 11 resistant strains, 9 strains transferred parts of their resistance to AP or AP CP or SM AP, indicating 9 strains carried R plasmids determining R(AP), R(AP CP) and R(SM AP). The conjugal frequency of R(AP) from Lactobacillus to E. coli ranged from 2.5{\times}10^{-1} to $5.6{\times}10^{-4}%$, that of R(CP) ranged from 5.0{\times}10^{-1} to 5.0{\times}10^{-3}% and that of R(SM) ranged from 6.0{\times}10^{-5} to 1.4{\times}10^{-5}%, at $37^{\circ}C$ for 18 hours of incubation.

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유산균음료(乳酸菌飮料)로부터 분리(分離)한 유산간균(乳酸桿菌)의 R-Plasmids의 중개(仲介)에 의(依)한 대장균(大腸菌)에로의 항생제내성(抗生劑耐性) 전달(傳達) (Drug Resistance and R Plasmids of Lactobacilli Isolated from Fermented Milk)

  • 하대유;이정호
    • 대한미생물학회지
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    • 제15권1호
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    • pp.55-62
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    • 1980
  • 시판(市販)되고 있는 9종(種)의 유산균음료(乳酸菌飮料)로부터 Lactobacillus bulgaricus 3주(株), Lactobacillus plantarum 2주(株), Lactobacillus cellobiosus 2주(株) 및 Lactobacillus lactis, Lactobacillus casei subsp. casei, Lactobacillus casei subsp, tolerans를 각각(各各) 1주(株) 분리(分離)하여 streptomycin(SM), chloramphenicol(CP), tetracyline(TC), penicillin(PC), ampicillin(AP), kanamycin(KM), erythromycin(EM) 및 nalidixic acid(NA) 등(等) 8종(種)의 약제(藥劑)에 대(對)한 내성검사(耐性檢査)와 접합(接合)(conjugation)에 의(依)한 대장균(大腸菌)에로의 내성인자(耐性因子)의 전달여부(傳達與否) 및 그 빈도(頻度)를 검사(檢査)하여 아래와 같은 성적(成績)을 얻었다. 전분리균주(全分離菌株)가 TC, PC 및 EM에는 고도(高度)의 감수성(感受性)을 보였으나, SM, CP, AP, KM 및 NA에는 대부분(大部分)이 중도(中度) 또는 고도(高度)의 내성(耐性)을 보였다. 분리균주(分離菌株)의 약제내성유형(藥劑耐性類型)은 NA AP(1주(株)), NA CP(1주(株)), NA AP CP(1주(株)), NA AP CP KM(2주(株)), NA AP CP SM(1주(株)) 및 NA AP CP SM KM(5주(株)) 등(等) 6종(種)으로서 전분리균주(全分離菌株)가 2제(劑) 이상(以上) 다제내성(多劑耐性)을 보였다. 분리균주(分離菌株)의 내성인자전달유형(耐性因子傳達類型)은 R(AP)가 6주(株)로서 대부분(大部分)이 1제내성(劑耐性)을 전달(傳達)하였으며, R(AP SM)형(型)도 3주(株)를 점(占)하였다. 분리균주(分離菌株)의 내성인자전달빈도(耐性因子傳達頻度)는 분리균주(分離菌株) 또는 약제(藥劑)에 따른 차이(差異)는 없었으며, 그 범위(範圍)는 $2.8^{-5}-1.5{\times}10^{-1}%$였다. 이상(以上)의 결과(結果)로 세균(細菌)과 세균(細菌)의 접촉(接觸)에 의(依)해서 유산간균(乳酸桿菌)으로부터 대장균(大腸菌)에로 내성인자(耐性因子)가 감염적(感染的)으로 전달(傳達)됨을 알 수 있었으며 유산균제제(乳酸菌製劑) 및 발효유(醱酵乳)에 사용(使用)되는 유산균주(乳酸菌株)의 선택(選擇)에 신중(愼重)해야 하리라고 사료(思料)되었다.

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젖산발효에 의한 혼합과채음료 제조의 최적화 (Optimization for the Lactic Acid Fermentation of Mixed Fruit and Vegetable Juices)

  • 김수연;최언호
    • 한국식품과학회지
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    • 제34권2호
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    • pp.303-310
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    • 2002
  • 당근, 사과, 샐러리, 돌미나리, 대추, 구기자의 착즙액을 3 : 3 : 1 : 1/2 : 1 : 1/2의 비율로 혼합한 혼합과채즙에 동치미로부터 분리한 Leuconostoc mesenteroides subsp. mesenteroides, Lactococcus lactis subsp. lactis. Lactobacillus cellobiosus을 접종하고 당과 식염의 첨가와 배양온도의 최적조건을 발효 및 관능특성에 중점을 두고 조사하였다. 올리고당을 포함한 당은 25% 이하에서, 식염은 0.8% 이하에서 젖산균의 성장률이나 산 생선능에 크게 영향을 미치지 않았다. 배양온도가 높을수록 젖산균수와 산도가 증가하고 대장균수와 당도가 빠르게 감소하였다. $15^{\circ}C$에서는 발효가 진행됨에 따라 점질물이 형성되어 발효쥬스의 선호도가 저하되었다. 따라서 혼합과채즙의 젖산발효는 관능특성면에서 fructooligosaccharide 15%, 식염 0.2%, 배양온도 $25^{\circ}C$가 최적이었고 최적 발효종점은 3일로서 이때의 pH는 3.62였다. 최적상태에서 발효쥬스는 1 mL당 젖산균수 8, 효모수 4의 지수를 보였으며 발효초기에 $5.6{\times}10^2\;cfu/mL$이던 대장균은 검출되지 않았다.