• Title, Summary, Keyword: antimicrobial activity

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Analogues of Hybrid Antimicrobial Peptide, CAMA-P2, Designed with Improved Antimicrobial and Synergistic Activities

  • Jeong, Ki-Woong;Shin, So-Young;Kim, Jin-Kyoung;Kim, Yang-Mee
    • Bulletin of the Korean Chemical Society
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    • v.32 no.8
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    • pp.2577-2583
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    • 2011
  • We have designed a 20-residue hybrid peptide CA(1-8)-MA(1-12) (CAMA) incorporating residues 1-8 of cecropin A (CA) and residues 1-12 of magainin 2 (MA) with high bacterial cell selectivity. CAMA-P2 is an ${\alpha}$-helical antimicrobial peptide designed from a CAMA hybrid peptide and substitution of Gly-Ile-Gly hinge sequence of CAMA to Pro influences the flexibility at central part of CAMA. Based on structure-activity relationships of CAMA peptides, to investigate the effects of the total positive charges on antimicrobial activity of CAMA-P2, the $Ser^{14}{\rightarrow}$Lys analogue (CAMA-syn1) was synthesized. The role of tryptophan at C-terminal ${\alpha}$-helix on its antimicrobial activity as well as synergistic activity was also investigated using $Ser^{14}{\rightarrow}$Lys/$Phe^{18}{\rightarrow}$Trp analogue (CAMA-syn2). Also, we designed CAMA-syn3 by substitution of $Lys^{16}$ located opposite side of substituted $Lys^{14}$ of CAMA-syn1 with Leu residue, resulting in increase of hydrophobicity and amphipathicity of the peptide. All of CAMA-syn analogues showed good antimicrobial activities similar to those of CAMA and CAMA-P2. The CAMA-syn1 and CAMA-syn2 showed low hemolytic activity and cytotoxicity against human keratinocyte Haca-T cells while CAMA-syn3 showed hemolytic activity and cytotoxicity at its MIC value. We then investigated their abilities to act synergistically in combination with the antimicrobial flavonoids and synthetic compounds screened in our laboratory. The results showed that all peptides exhibited synergistic effects with dihydrobinetin, while only CAMA-syn2 exhibited synergistic effects with YKAs3001 against both S. aureus and MRSA, suggesting that Trp residue at C-terminus of CAMA-syn2 may facilitate the polar antibiotic flavonoids and synthetic compounds to permeabilize the membrane. This study will be useful for the development of new antibiotic peptides with potent antimicrobial and synergistic activity but without cytotoxicity.

Study of Antimicrobial Activity of New Zealand’s Tea Tree Essential Oil , Grapefruit Seed Extract and its major Component.

  • Han, Chang-Giu;Lee, Young-Woon;Zhoh, Choon-Koo;Kim, Byung-Hoon
    • Proceedings of the SCSK Conference
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    • pp.17-41
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    • 1999
  • Manuka oil sometime named New Zealand's tea tree oil is soluble in oil and come from nature. The $\alpha$-pinene extracted from Manuka oil and R-limonene which is one of the component of extracted Citrex from Grapefruit were used to estimate the antimicrobial activity and to improve the capability of antiseptic. Disk diffusion and broth dilution methods were used to measure the antimicrobial activity Escherichia coli which is gram-negative bacteria and Staphylococcus aureus which is gram-positive bacteria were used as strain. The antimicrobial activity of Manuka oil and $\alpha$-pinene for Escherichia coli, Staphylococcus aureus is similar when the concentration of Manuka oil and $\alpha$-pinene is 10${mu}ell$. However, Antimicrobial activity of Manuka oil for EscherEchta coli, Staphylococcus aureus is better than that of $\alpha$-pinene when the concentration of Manuka oil and $\alpha$-pinene is low. Antimicrobial activity of Citrex is superior to that of R-limonene. The proper ratio of Maunka oil and Citrex can Improve the antimicrobial activity. The proper ratio obtained from studies was 75% of Maunka oil and 25% Citrex for Escherichia coli, 25% of Maunka oil and 75% Citrex for Staphylococcus aureus.

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Antimicrobial Activity of Areca catachu L. Extract of against Intestinal Pathogens (장내 유해세균에 대한 빈랑 추출물의 항균 활성)

  • 이갑상;김성효;전승호;박성수;박정순;신용서
    • The Korean Journal of Food And Nutrition
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    • v.11 no.1
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    • pp.36-40
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    • 1998
  • Areca catachu L. which was showed antimicrobial activity against intestinal pathogens through screening herbs related treatments of intestinal diseases, were extracted by methanol and fractionated by n-hexane, ethylether, ethylacetate, and water. Antimicrobial activities of the methanol extract and each fractionates were then investigated under the anaerobic broth system. All fractions of Areca catachu L. except of n-hexane showed antimicrobial activity against all tested pathogens. Especially, ethylacetate fraction of them had the most significant inhibition activity. There is no significant difference of antimicrobial activity among each fractionates. Fraction of Areca catachu L. ethylacetate fractionate, which were fractionated by Sephadex G-200 and Silica gel column chromatography revealed the strongest antimicrobial activity at 5 to 7 and 20 of fraction number, respectively.

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Study of Antimicrobial Activity of New Zealand's Tea Tree Essential Oil, Grapefruit Seed Extract and its major Component.

  • Han, Chang-Giu;Lee, Young-Woon;Zhoh, Choon-Koo;Kim, Byung-Hoon
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.25 no.4
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    • pp.17-41
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    • 1999
  • Manuka oil sometime named New Zealand's tea tree oil is soluble in oil and come from nature. The $\alpha$-pinene extracted from Manuka oil and R-limonene which is one of the component of extracted Citrex from Grapefruit were used to estimate the antimicrobial activity and to improve the capability of antiseptic. Disk diffusion and broth dilution methods were used to measure the antimicrobial activity. Escherichia coli which is gram-negative bacteria and Staphylococcus aureus which is gram-positive bacteria were used as strain. The antimicrobial activity of Manuka oil and $\alpha$-pinene for Escherichia coli, Staphylococcus aureus is similar when the concentration of Manuka oil and $\alpha$-pinene is $10{\mu}l$. However, Antimicrobial activity of Manuka oil for Escherichia coli, Staphylococcus aureus is better than that of $\alpha$-pinene when the concentration of Manuka oil and $\alpha$-pinene is low. Antimicrobial activity of Citrex is superior to that of R-limonene. The proper ratio of Maunka oil and Citrex can improve the antimicrobial activity. The proper ratio obtained from studies was 75% of Maunka oil and 25% Citrex for Escherichia coli, 25% of Maunka oil and 75% Citrex for Staphylococcus aureus.

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Antimicrobial Activity of the Cell Organelles, Lysosomes, Isolated from Egg White

  • Yoon, Ji-Hee;Park, Jae-Min;Kim, Ki-Ju;Kim, Yang-Hoon;Min, Ji-Ho
    • Journal of Microbiology and Biotechnology
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    • v.19 no.11
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    • pp.1364-1368
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    • 2009
  • Lysosomes, as a cell organelle type, are safe biological control agents that may be possible replacements for chemical antimicrobial agents because they are simply isolated from egg white. In this study, it was found that the lysosomes isolated from egg white exhibited pH-dependent antimicrobial activity, with the optimal activity found at pH 6.0. The efficiency of lysosomes in inhibiting bacterial growth and activity was evaluated over a 12-h treatment period. Seven different microorganisms were used as bacterial strains, and the lysosomes showed a significant antimicrobial effect against all strains. In addition, the antimicrobial activity was maintained for 100 days, and there did not appear to be any resistance of E. coli to the lysosomal activity up to the eighth culture. However, the lysosomes did not affect the viability of mammalian cells, suggesting the biocompatibility of lysosomes. These highly effective lysosomes have a bright future in the application of novel antimicrobial sources as a cell organelle type.

Antimicrobial Characterictics of Antimicrobial Agent (Antibiotics) and Reduction Effect on Mal-ordour. (항균제의 항균특성 및 악취제거 효과)

  • Shin, Choon-Hwan;Kim, Jong-Hyun;Han, Sun-Hong
    • Journal of Environmental Science International
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    • v.3 no.2
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    • pp.157-164
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    • 1994
  • Various antimicrobial agents are widely used for the purpose of antimicrobial process. We investigated antimicrobial activity and reduction efficiency of mal-ordour by the diphenyl ether compound (2,4,4'- trichloro -2'- hydroxy diphenyl ether) against Sraphylocom aureus(S.aureus and Proton vulgaris(p.vulgaris causing the mal-ordour, Especially, the diphenyl ether compound is not restricted to the regulation of water-contamination. In this research, we found that the optimum concentration of diphenyl ether compound was 1.5w% for both strains and antimicrobial expressions were c0.38t= 2.56 for S.aureus, c0.38t=2.67 for P.vulgaris. We found also that -OH group played the role of antimicrobial functional group. Lastly, reduction effect of mal-ordour was more than 90% for both strain at the optimum conditions. Key Words : antimicrobial agents, antimicrobial activity, reduction effect of mal-ordour, antimicrobial expression, antimicrobial functional group.

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Antibacterial Activity of Recombinant Pig Intestinal Parasite Cecropin P4 Peptide Secreted from Pichia pastoris

  • Song, Ki-Duk;Lee, Woon-Kyu
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.2
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    • pp.278-283
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    • 2014
  • Cecropins (Cec) are antibacterial peptides and their expression is induced in a pig intestinal parasite Ascaris suum by bacterial infection. To explore the usefulness of its activity as an antibiotic, CecP4 cDNA was prepared and cloned into the pPICZ B expression vector and followed by the integration into AOX1 locus in Pichia pastoris. The supernatants from cell culture were collected after methanol induction and concentrated for the test of antimicrobial activity. The recombinant P. patoris having CecP4 showed antimicrobial activity when tested against Staphyllococcus aureus in disc diffusion assay. We selected one of the CecP4 clones (CecP4-2) and performed further studies with it. The growth of recombinant P. pastoris was optimized using various concentration of methanol, and it was found that 2% methanol in the culture induced more antibacterial activity, compared to 1% methanol. We extended the test of antimicrobial activity by applying the concentrated supernatant of CecP4 culture to Pseudomonas aeruginosa and E. coli respectively. Recombinant CecP4 also showed antimicrobial activity against both Pseudomona and E. coli, suggesting the broad spectrum of its antimicrobial activity. After improvements for the scale-up, it will be feasible to use recombinant CecP4 for supplementation to the feed to control microbial infections in young animals, such as piglets.

A Study on the Improvement of Antimicrobial Activity and Crease Resistance of Korean Traditional Hansan Ramie Fabrics (한국 전통한산모시의 항균성 및 방추도 향상에 관한 연구)

  • Kim, Ho Jung;Choi, Jae Hyeok;Kim, Han Do;Park, Cha Cheol
    • Textile Coloration and Finishing
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    • v.6 no.4
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    • pp.1-8
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    • 1994
  • To improve the antimicrobial activity and crease resistance of Korean traditional Hansan ramie fabrics, partially saponified polyethylene(PPS) was used as a softening water repellent agent and 3-(trimethoxysilyl)propyl dimethyl octadecyl ammonium chloride(QAC) as a antimicrobial agent. The antimicrobial activity was effective by treatments of PPS, QAC and PPS/QAC for shake flask test. For the halo test, PPS/QAC showed good antimicrobial activity. It was found that the desirable composition of H$_{2}$O/PPS/QAC is 98/1/1 for improving the antimicrobial activity and crease resistance of the fabrics.

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Characteristics of Sophorolipid as an Antimicrobial Agent

  • KIM, KAPJUNG;DALSOO YOO;YOUNGBUM KIM;BAEKSEOK LEE;DOONHOON SHIN;EUN-KI KIM
    • Journal of Microbiology and Biotechnology
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    • v.12 no.2
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    • pp.235-241
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    • 2002
  • Sophorolipid, a biosurfactant produced from Candida bombicola ATCC 22214, showed antimicrobial activity against Bacillus subtilis, Staphylococcus xylosus, Streptococcus mutans, and Propionibacterium acne at 4, 1, 1, 0.5 ppm, respectively. Also, 100 ppm of sophorolipid inhibited $50\%$ of cell growth of plant pathogenic fungus, Botrytis cineria. However, sophorolipid showed no effect on Escherichia coli, indicating that its selective antimicrobial activity depended on the cell wall structure. Treatment of B. subtilis with sophorolipid increased leakage of intracellular enzyme, malate dehydrogenase, indicating a possible interaction of sophorolipid with a cellular membrane. Comparing lactone-type and acid-type sophorolipids, the former showed a higher antimicrobial activity. Supplementing other surfactants showed no significant effects on the antimicrobial activity. Animal study showed that 5 g of sophorolipid per kg body weight by oral administration caused no toxicity, and sophorolipid induced no irritation on the skin. These results show potential use of sophorolipid as an active ingredient in healthcare products.

Antimicrobial Effect of Sophora angustifolia Extracts on Food-Borne Pathogens

  • Bae, Ji-Hyun
    • Food Science and Biotechnology
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    • v.14 no.3
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    • pp.311-316
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    • 2005
  • This study was performed to investigate the antimicrobial effect of Sophora angustifolia extracts against food-borne pathogens. First, Sophora angustifolia was extracted with methanol at room temperature, and the methanol extracts from Sophora angustifolia were fractionated using petroleum ether, chloroform, ethyl acetate, and methanol. The antimicrobial activity of the Sophora angustifolia extracts was determined using the paper disc method against food-borne pathogens and food spoilage bacteria. The methanol extracts of Sophora angustifolia showed the highest antimicrobial activity against Staphylococcus aureus and Salmonella typhimurium. A synergistic effect was found in the combined extracts of Sophora angustifolia and Portulaca oleracea, compared to the activity of each extract alone. Finally, the growth inhibition curve was determined using the methanol extracts of Sophora angustifolia against Staphylococcus aureus and Salmonella typhimurium. The methanol extract of Sophora angustifolia showed strong antimicrobial activity against Staphylococcus aureus at a concentration of 5,000 ppm. The 5,000 ppm methanol extract from Sophora angustifolia retarded the growth of S. aureus for more than 24 hours and of Salmonella typhimurium for up to 12 hours.