• Title/Summary/Keyword: cytoprotective effect

Search Result 198, Processing Time 0.024 seconds

Cytoprotective Effect of Phenolic Compounds Against Hexavalent Chromium-Induced Cytotoxicity (페놀 화합물의 Hexavalent Chromium독성에 대한 세포 보호효과)

  • Han, Du-Seok;Kang, Jeong-Il;Baek, Seung-Hwa
    • Journal of Society of Preventive Korean Medicine
    • /
    • v.13 no.2
    • /
    • pp.1-18
    • /
    • 2009
  • Objectives : In order to evaluate the cytotoxicity of hexavalent chromium, the cytoprotective effect of phenolic compounds against hexavalent chromium-induced cytotoxicity, cell viability, cell adhesion ability, lactate dehydrogenase(LDH) activity, and morphological changes of cells were examined. Methods : We measured the cytotoxicity of hexavalent chromium with 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide(MTT), 2,3-bis-[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-caboxanilide (XTT), LDH and DPPH methods. Results : The cytotoxicity of hexavalent chromium($IC_{50}$, $44.0-51.0{\mu}M$) was high according to the toxic criteria. Cytoprotective effect of phenolic compounds against $IC_{50}$ value of hexavalent chromium in cell morphology increased in a concentration-dependent manner. Conclusions : These results suggest that 3,4,5-trihydroxybenzoic acid may be used as a cytoprotective agent against chromium(IV)-mediated cytotoxicity.

  • PDF

Antioxidative and Cytoprotective Effects of Isoflavones Isolated from Pueraria thunbergiana Flowers (갈화에서 분리한 이소플라본의 항산화 및 세포보호효과)

  • 이경태;손일철;공은아;김동현;최승기;최종원;박희준
    • YAKHAK HOEJI
    • /
    • v.43 no.6
    • /
    • pp.736-742
    • /
    • 1999
  • Antioxidative and cytoprotective effects of tectorigenin and glycitein isolated from the pueraria thunbergiana and its derivative, genistein, were determined. Among these three compounds, tectorigenin and glycitein bearing 6-methoxyl groups in both isoflavones showed significant free radical scavenging activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and xanthine/xanthine oxidase (XOD) generating superoxide anion radical. Tectorigenin only showed a slight inhibitory effect on XOD. We further studied the inhibitory effects of these isoflavones on the lipid peroxidation in rat liver microsomes induced by enzymatic and non-enzymatic methods. Each of them exhibited inhibitory effect on both ascorbic $acid/Fe^{2+}-{\;}and{\;}ADP/NADPH/Fe^{+3}-induced$ lipid peroxidation. Moreover, tectorigenin exhibited the highest protection of hydrogen peroxide damage on HepG2 and Vero cells among the three isoflavones, in the cytoprotective assay. It was suggested that the pattern of antioxidative and cytoprotective effect of isoflavones could be crucially by the aromatic substitution of oxygen-containing groups.

  • PDF

Evaluation of Antioxidant, Cytoprotective and Antimicrobial Activities of the Extract and Fractions Obtained from Young Shoots of Nypa Fruticans Wurmb (니파야자(Nypa fruticans Wurmb) 싹 추출물 및 분획물의 항산화, 세포 보호 및 항균 효과에 관한 평가)

  • Shin, Hyuk Soo;Lee, Yoon Joo;Kim, Ji Woong;Song, Ba Reum;Lee, Sang Lae;Park, Soo Nam
    • Korean Journal of Pharmacognosy
    • /
    • v.49 no.2
    • /
    • pp.155-164
    • /
    • 2018
  • Nypa fruticans Wurmb is a species of palm, which is widely distributed in the mangrove forest of Southeast Asia. Various parts of N. fruticans has been used as a traditional medicinal plant. However, the physiological activities of N. fruticans has not yet been clarified well. Therefore, in this study, the 50% ethanol extract and its aqueous and ethyl acetate fractions of young shoots of N. fruticans were investigated for their antioxidant, cytoprotective effect, and antimicrobial activities. Every sample possessed very high free radical and various ROS scavenging capacities assessed by employing different in vitro assays such as $DPPH^{\cdot}$, $O_2^{{\cdot}-}$, ${\cdot}OH$, and $^1O_2$ scavenging activities. Based on these results, the cytoprotective effect was investigated using the oxidative hemolysis of erythrocyte. We found that the extract and fractions provide a greater protective effect compared with (+)-${\alpha}$-tocopherol. Furthermore, antimicrobial activities were confirmed against skin pathogens by broth microdilution assay. The ethyl acetate fraction had much higher antimicrobial activities than methyl paraben against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans. Taken together, our results indicated that the young shoots of N. fruticans may have the potential role as a natural active ingredient through their antioxidant, cytoprotective effect, and antimicrobial activities.

Depigmentation Activity of Barley, Unpolished Rice, Job's-tear (보리·현미·율무의 미백효과에 관한 연구)

  • Lee, Tae-huen;Kim, Hye-jeong;Kim, Yoon-bum
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
    • /
    • v.16 no.2
    • /
    • pp.57-77
    • /
    • 2003
  • Objectives: The aim of this study was to investigate the depigmentation effects of Barley, Unpolished rice, Job's-tear. Metbods: We investigated that the extracts of Barley, Unpolished rice, Job's-tear inhibit activity of tyrosinase, the enzyme which convert ts 3-(3,4-dihydroxyphenyl) alanine to dopachrom in the biosynthetic process of melanin, the UV absorbance of the extracts in the UV-A region and UV-B region was measured by UV scanning, the effect of extracts on cell viability and melanin production in cultured B16 mouse melanoma cells were measured, and cytoprotective effects of extracts on PC12 cells injured by hydrogen peroxide measured by MTT assay. Results: The extracts of Barley, Unpolished rice, Job's-tear inhibited activity of tyrosinase in low density. The Barley, Job's-tear extracts not only showed inhibitory effects on melanin production in cultured B16 mouse melanoma cells, but also exhibited cytoprotective effects on PC12 cells injured by hydrogen peroxide in low density. Unpolished rice extract showed inhibitory effect on melanin production in cultured B16 mouse melanoma cells, but did not showed cytoprotective effect Barley, Unpolished rice, Job's-tear extracts did not showed an absorbance effect in the UV-A region and UV-8 region. Conclusions: These results suggest that Barley, Unpolished rice, Job's-tear inhibit melanin biosynthesis which is involved in hyperpigmentation and could be used as a whitening agent.

  • PDF

Cytoprotective Constituents of the Stem Barks of Fraxinus rhynchophylla on Mouse Hippocampal HT22 Cells and Their Antioxidative Activity (물푸레나무 수피의 생쥐 해마 유래 HT22 세포 보호와 항산화 활성 물질)

  • Jeong, Gil-Saeng;Yoon, Kwon-Ha;Kim, Hyun-Chul;Oh, Seung-Hwan;Kim, Myong-Jung;Kang, Dae-Gill;Lee, Ho-Sub;Kim, Youn-Chul
    • Korean Journal of Pharmacognosy
    • /
    • v.38 no.3 s.150
    • /
    • pp.287-290
    • /
    • 2007
  • Phytochemical investigation of the MeOH extract of the dried stem barks of Fraxinus rhynchophylla Hance (Oleaceae), as guided by cytoprotective activity against tert-butyl hydroperoxide (t-BHP)-induced cell injury in mouse hippocampal HT22 cells, furnished two coumarins, esculetin (1) and fraxetin (2). Compounds 1 and 2 had the significant cytoprotective effects on t-BHP-induced cellular oxidative injury in HT22 cells. Furthermore, compounds 1 and 2 showed potent DPPH radical scavenging effect, exhibiting $IC_{50}$ values of 14.68 and 9.64 ${\mu}M$, respectively.

Cytoprotective Effect of Galgeuntang Extract on Cadmium-Induced Cytotoxicity (갈근탕 추출물의 카드뮴 독성에 대한 세포 보호효과)

  • Park, So-Yun;Baek, Jong-Min;Baek, Seung-Hwa
    • YAKHAK HOEJI
    • /
    • v.54 no.3
    • /
    • pp.151-156
    • /
    • 2010
  • Cytotoxicity of cadmium on NIH 3T3 fibroblasts was utilized in order to discover antitoxic compound in Galgeuntang extract in this study. Treatment groups were chosen as follows; control (medium only), $MTT_{50}$ group and five experimental groups. MTT {3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide} method was performed to evaluate the cytotoxicity of cell organelles and $IC_{50}$ was also measured. Accordingly we have examined the detoxification effects of Galgeuntang extract on cadmium-treated NIH 3T3 fibroblasts to observe morphological changes by the light microscopy. Galgeuntang extract showed cytoprotective effects on cadmium-induced cytotoxicity. Furthermore, Galgeuntang showed a dose-dependency in detoxication. The phenolic content of Galgeuntang ethanol extract was higher than that of water content. These results suggest that Galgeuntang extract may be used as a cytoprotective agent against cadmium (II)-mediated cytotoxicity.

The Study on Depigmentation of Kamibangpungtongsung-San (加味防風通聖散의 美白效果에 관한 硏究)

  • Lee Seung-eun;Kim Hae-jeong;Kim Yoon-bum
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
    • /
    • v.17 no.1
    • /
    • pp.94-103
    • /
    • 2004
  • Objective: This study was performed to detennine the depigmenting effects of Kamibongpungtongsung-San. Methods: To determine the depigmenting effects of Kamibangpungtongsung-San. we measured the degree of tyrosinase inhibition, melanin production & cell viability in cultured B16 melanoma cells, UV screen and cytoprotective effects on PC12 cells injured by hydrogen peroxide. Results: Komibangpungtongsung-San did not show inhibitory effects on melanin production in melanoma cells, UV screen and cytoprotective effects on PC12 cells injured by hydrogen peroxide. However it showed mild inhibitory effects on tyrosinase activity. Conclusion : This study shows that Kamibangpungtongsung-San, a generally used prescription for dermatologic diseases, do not have depigmenting effects via tyrosinase inhibition. Therefore, the depigmenting effect and mechanism of depigmentation by Kamibangpungtongsung-San need to be evaluated and investigated in other directions.

  • PDF

Cytoprotective effects of eupatilin, a novel antioxidative flavone, in oxidative stress- induced gastric mucosal cell damage

  • Oh, Tae-Young;Kim, Ju-Mi;Ahn, Byoung-Ok;Kim, Won-Bae;Park, Eun-Joo;Kim, Yong-Seok;Lee, Zee-Won;Ha, Kwon-Soo
    • Proceedings of the PSK Conference
    • /
    • 2003.04a
    • /
    • pp.129.1-129.1
    • /
    • 2003
  • Alcohol, Helicobacter pylori, stress and NSAIDs-activated neutrophils all produce reactive oxygen species (ROS), which play an important role in gastric mucosal damage. Eupatilin is an active component of Artemisia asiatica possessing cytoprotective effect. The effect of eupatilin on the production of ROS and cellular damage in AGS and ECV304 cells were evaluated to prove the cytoprotective action against the above mentioned gastric mucosal cell damages. (omitted)

  • PDF

Sphigosine-1-Phosphate-Induced ERK Activation Protects Human Melanocytes from UVB-Induced Apoptosis

  • Kim, Dong-Seok;Kim, Sook-Young;Lee, Jai-Eun;Kwon, Sun-Bang;Joo, Young-Hyun;Youn, Sang-Woong;Park, Kyoung-Chan
    • Archives of Pharmacal Research
    • /
    • v.26 no.9
    • /
    • pp.739-746
    • /
    • 2003
  • Ultraviolet B (UVB) is known to induce apoptosis in human melanocytes. Here we show the cytoprotective effect of sphingosine-1-phosphate (S1P) against UVB-induced apoptosis. We also show that UVB-induced apoptosis of melanocytes is mediated by caspase-3 activation and poly(ADP-ribose) polymerase (PARP) cleavage, and that S1P prevents apoptosis by inhibiting this apoptotic pathway. We further investigated three major mitogen-activated protein (MAP) kinases after UVB irradiation. UVB gradually activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, while extracellular signal-regulated protein kinase (ERK) was inactivated transiently. Blocking of the p38 MAP kinase pathway using SB203580 promoted cell survival and inhibited the activation of caspase-3 and PARP cleavage. These results suggest that p38 MAP kinase activation may play an important role in the UVB-induced apoptosis of human melanocytes. To explain this cytoprotective effect, we next examined whether S1P could inhibit UVB-induced JNK and p38 MAP kinase activation. However, S1P was not found to have any influence on UVB-induced JNK or p38 MAP kinase activation. In contrast, S1P clearly stimulated the phosphorylation of ERK, and the specific inhibition of the ERK pathway using PD98059 abolished the cytoprotective effect of S1P. Based on these results, we conclude that the activation of p38 MAP kinase plays an important role in UVB-induced apoptosis, and that S1P may show its cytoprotective effect through ERK activation in human melanocytes.

Cytoprotective effect of Eriobotrya japonica L. against the iron-induced oxidative stress through AMPK activation (AMPK 활성화를 통한 중금속 유발 산화적 스트레스에 대한 비파엽의 세포 보호 효과)

  • Min-Jin Kim;Young-Eun Kim;Seon Been Bak;Su-Jin Bae;Kwang-Il Park;Sun-Dong Park;Young Woo Kim
    • Herbal Formula Science
    • /
    • v.32 no.1
    • /
    • pp.99-109
    • /
    • 2024
  • Objectives : In this study, we investigated the cytoprotective effect of Eriobotrya japonica L. (EJ) extract against Arachidonic acid (AA)+iron-induced oxidative stress. Methods : To confirm the cytoprotective effect of EJ against AA+iron-induced oxidative stress in HepG2 cells, it was evaluated by MTT assay, immunoblot anaylsis, and Calcein-AM/propidium iodide (PI) staining. Additionally, the mechanism of action of the cytoprotective effect was evaluated through molecular mechanisms. Results : EJ (100 ㎍/mL) inhibited Arachidonic acid (AA)+iron-induced cell death in a concentration-dependent manner. It also inhibited AA+iron-induced mitochondrial dysfunction and ROS production. EJ activated the LKB1-AMPK signaling pathway. Conclusions : In conclusion, EJ has the ability to protect liver cells from oxidative stress, indicating that it is related to AMPK-LKB1 signaling pathways.