• BMB Reports
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• v.48 no.11
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• pp.595-596
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• 2015

Skeletal muscle exhibits a loss of muscle mass and function with age. Decreased regenerative potential of muscle stem/progenitor cells is a major underlying cause of sarcopenia. We analyzed microRNAs (miRNA) that are differentially expressed in young and old myoblasts, to identify novel intrinsic factors that play a degenerative role in aged skeletal muscle. miR-431, one of decreasing miRNAs in old myoblasts, improved the myogenic differentiation when overexpressed in old myoblast, but suppressed their myogenic capability in knockdowned young myoblasts. We found that miR-431 directly binds to 3` untranslated regions (UTR) of Smad4 mRNA, and decreases its expression. Given that SMAD4 is one of the downstream effectors of TGF-β, a well-known degenerative signaling pathway in myogenesis, the decreased miR-431 in old myoblast causes SMAD4 elevation, thus resulting in defective myogenesis. Exogenous expression of miR-431 greatly improved the muscle regeneration in the cardiotoxin-injured hindlimb muscle of old mice by reducing SMAD4 levels. Since the miR-431 seed sequence is conserved in human SMAD4 3'UTR, miR-431 regulates the myogenic capacity of human skeletal myoblasts in the same manner. Our results suggest that age-associated miR-431 is required for the maintenance of the myogenic capability in myoblasts, thus underscoring its potential as a therapeutic target to slow down muscle aging.

• The Journal of the Korea Contents Association
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• v.21 no.11
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• pp.638-645
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• 2021

This study was attempted to find out the effect of biomedical ethics and critical thinking disposition of nursing college students on professionalism. For this study, from May to June 2021, 144 students were participated from S University in A City, using self reported questionnaire. Descriptive statistics, t-test, ANOVA, Scheffe test, Pearson's correlation analysis, and multiple regression analysis were used for data analysis. As results of this study, it was revealed that there was a significant positive correlation between the professionalism of nursing students on biomedical ethics and critical thinking disposition(r=.400, p<.001, r=.431, p<.001). Also, the factors affecting the professionalism of nursing students were grade(𝛽=0.303, p<.001), biomedical ethics(𝛽=0.287, p<.001) and critical thinking disposition(𝛽=0.294, p<.001) with a total explanatory power of 31.2%. As the results of this study, in order to increase the professionalism of nursing students, it is necessary to develop their biomedical ethics and critical thinking disposition. The specific strategies are to make and apply special programs by their grades.

• Ocean and Polar Research
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• v.27 no.4
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• pp.419-431
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• 2005

With the draining of land resources, and rising new economic and resources expansionism, finding a new and yet to be developed ${\ulcorner}treasure{\lrcorner}$ is often a fierce and competitive challenge among many nations. This is especially true for Korea confronted with its urgent needs to find new resources and strategy to maintain the current pace of societal demand and economic development in this often impatient and rapidly progressing world. The ocean world in the South Pacific provides potentials to quench both orders with promises of new ${\ulcorner}treasure{\lrcorner}$ of living and ecosystem resources such as new fisheries and new bioactive products as well as providing alternative aesthetic, recreational resources and critical informations on various societal problems such as extreme weather conditions and early wining of the consequences resulting from global environmental changes.

• Journal of Gastric Cancer
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• v.17 no.4
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• pp.384-393
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• 2017

Purpose: The tumor microenvironment is known to be associated with the metabolic activity of cancer cells and local immune reactions. We hypothesized that glucose metabolism measured by 2-deoxy-2-($^{18}F$)fluoro-D-glucose ($^{18}F-FDG$) positron emission tomography (PET)-computed tomography (CT) ($^{18}F-FDG$ PET-CT) would be associated with local immune responses evaluated according to the presence of tumor infiltrating lymphocytes (TILs). Materials and Methods: We retrospectively reviewed 56 patients who underwent $^{18}F-FDG$ PET-CT prior to gastrectomy. In resected tumor specimens, TIL subsets, including cluster of differentiation (CD) 3, CD4, CD8, Forkhead box P3 (Foxp3), and granzyme B, were subjected to immunohistochemical analysis. The prognostic nutritional index (PNI) was calculated as: ($10{\times}serum$ albumin value)+($0.005{\times}peripheral$ lymphocyte counts). Additionally, the maximum standard uptake value ($SUV_{max}$) was calculated to evaluate the metabolic activity of cancer cells. Results: The $SUV_{max}$ was positively correlated with larger tumor size (R=0.293; P=0.029) and negatively correlated with PNI (R=-0.407; P=0.002). A higher $SUV_{max}$ showed a marginal association with higher CD3 (+) T lymphocyte counts (R=0.227; P=0.092) and a significant association with higher Foxp3 (+) T lymphocyte counts (R=0.431; P=0.009). No other clinicopathological characteristics were associated with $SUV_{max}$ or TILs. Survival analysis, however, indicated that neither $SUV_{max}$ nor Foxp3 held prognostic significance. Conclusions: FDG uptake on PET-CT could be associated with TILs, especially regulatory T cells, in gastric cancer. This finding may suggest that PET-CT could be of use as a non-invasive tool for monitoring the tumor microenvironment in patients with gastric cancer.

• Journal of Microbiology and Biotechnology
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• v.25 no.4
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• pp.431-438
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• 2015

Ever since the ban on antibiotic growth promoters (AGPs), the livestock death rate has increased owing to pathogenic bacterial infections. There is a need of developing AGP alternatives; however, the mechanisms by which AGP enhances livestock growth performance are not clearly understood. In this study, we fed 3-week-old swine for 9 weeks with and without AGPs containing chlortetracycline, sulfathiazole, and penicillin to investigate the effects of AGPs on swine gut microbiota. Microbial community analysis was done based on bacterial 16S rRNA genes using MiSeq. The use of AGP showed no growth promoting effect, but inhibited the growth of potential pathogens during the early growth stage. Our results showed the significant increase in species richness after the stabilization of gut microbiota during the post-weaning period (4-week-old). Moreover, the swine gut microbiota was divided into four clusters based on the distribution of operational taxonomic units, which was significantly correlated to the swine weight regardless of AGP treatments. Taxonomic abundance analysis indicated a negative correlation between host weight and the abundance of the family Prevotellaceae species, but showed positive correlation to the abundance of the family Spirochaetaceae, Clostridiaceae_1, and Peptostreptococcaeae species. Although no growth performance enhancement was observed, the use of AGP inhibited the potential pathogens in the early growth stage of swine. In addition, our results indicated the ecological succession of swine gut microbiota according to swine weight. Here, we present a characterization of swine gut microbiota with respect to the effects of AGPs on growth performance.

• Proceedings of the KAIS Fall Conference
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• 2009.05a
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• pp.616-619
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• 2009

국내 육성 품종 JW07-190, JW07-192과 일본산 도입 품종 Meiryoku (MR), Ryoufuu (RF), Yamanoibuki (YI) 대해서 80% 메탄올 추출물을 공시 재료로 ESR을 이용하여 항산화 효능을 평가하였다. 국내 육성 품종인 JW07-192에서 DPPH radical, alkyl radical, hydroxyl radical에 대해 비교적 높은 radical 소거능을 보였고, 모든 녹차 품종에서 강력한 DPPH radical 소거능을 보였다. 전체적으로, 국내 육성 품종인 JW07-192가 일본 도입 품종과 비교하였을 때 더 좋은 항산화 효능을 나타내었다. 총 폴리페놀 함량은 국내 육성 계통인 JW07-192가 $431.57{\pm}28.45\;mg/g$로 가장 높은 함량을 나타냈고, 플라보노이드 함량은 도입 품종인 YI가 $313.44{\pm}6.31$로 가장 높은 함량을 보였다.

• Food Science and Preservation
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• v.16 no.6
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• pp.830-837
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• 2009

A total of 24 processed barley samples produced in Korea, 9 split and 15 pressed, were analyzed for protein, $\beta$-glucan, and amylose content whiteness cooking characteristics (water absorption and expansibility) and pasting properties, with respect to the areas in which they were grown. Split and pressed barley products from Japan were compared. Both forms of barley products had similar contents of protein and $\beta$-glucan. Whiteness was higher in pressed barley than in split barley. Split barley produced in Korea had a higher water absorption (342-436%) and expansibility (449-608%) than did pressed barley. Japanese processed barley products were much lower in protein (4.4-6.6%, w/w) and showed a higher whiteness grade than did Korean products. Whiteness of pressed barley ranged from 57.2-68.3 and was higher than that of split barley, but split barley product showed better cooking characteristics than did pressed barley. Japanese products were similar to Korean materials in this respect. A negative correlation was observed between protein content in and whiteness of processed barley products (r=-0.5112, p<0.01). Waxy barley products had a lower pasting temperature, and showed higher breakdown and lower setback than did non-waxy barley products. The pasting properties of Japanese products were different from those of Korean materials. The pasting temperatures of Japanese split and pressed barley were lower, and the setback much higher, than seen with Korean products. The results show that both splitting and pressing efficiently improve the cooking characteristics of barley. The processing of waxy barley was particularly effective.