Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Screening of Microorganisms Having ACAT Inhibitor Activity from Soil and Characterization of AI-3, ACAT Inhibitor Produced by Streptomyces sp. A-3

토양으로부터 ACAT 저해활성을 나타내는 미생물의 탐색과 방선균이 생산하는 ACAT 저해물질, AI-3의 특성

  • 정태숙 (한국과학기술연구원 유전공학연구소 생물소재연구 Group) ;
  • 김성욱 (한국과학기술연구원 유전공학연구소 생물소재연구 Group) ;
  • 이항우 (한국과학기술연구원 유전공학연구소 생물소재연구 Group) ;
  • 손광희 (한국과학기술연구원 유전공학연구소 생물소재연구 Group) ;
  • 권용욱 (한국과학기술연구원 유전공학연구소 생물소재연구 Group) ;
  • 최명언 (서울대학교 자연과학대학 화학과) ;
  • 복성해 (한국과학기술연구원 유전공학연구소 생물소재연구 Group)
  • Published : 1993.12.01
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Abstract

About 1, 300 strains isolated from soil were evaluated for acyl-CoA:cholesterol acyltransferase (ACAT) inhibition activity. About 4.0% of actinomycetes and 3.6% of fungi showed greater than 50% inhibition activity, respectively. However, none of the isolated bacteria exhibited inhibition activity more than 50%. Among them, one Streptomyces sp. A-3 showed a higher ACAT inhibition activity in culture broth. Isolation of the ACAT inhibitor (AI-3) was achieved by Amberlite XAD-7 column, silica-gel column, Sephadex LH-20 gel-filtration and reverse phase HPLC.

Keywords

References

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