YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effects of Bulbocapnine on Dopamine Content in PC12 Cells

Bulbocapnine이 PC12 세포중의 도파민 함량에 미치는 영향

  • Published : 1998.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

The effects of bulbocapnine, an aporphine isoquinoline alkaloid, on dopamine content in PC12 cells were investigated. Bulbocapnine decreased the dopamine content dose-dependentl y (39.2% inhibition at 2O${\mu}$M for 24 hr). The $IC_{50}$ value of bulbocapnine was 22.7${\mu}$M. Dopamine content was lowered at 6 hr after exposure to bulbocapnine. And then, the decreased dopamine level was almost maintained at 36 hr and recovered to the control level at about 60 hr. Tyrosine hydroxylase, the rate limiting enzyme in the catecholamine blosynthetic pathway, was also inhibited at 20${\mu}$M of bulbocapnine by 23.1% relative to control. We, therefore, hypothesized that the inhibition of tyrosine hydroxylase by bulbocapnine with a single treatment might be partially contributed to the decrease in dopamine content in PC12 cells.

Keywords

References

  1. Free Radical. Biol. Med. v.12 Inhibition of nonenzymic lipid peroxidation by benzylisoquinoline alkaloids artinez, L.A.;Rios, J.L.;Paya, M.;Alcaraz, M.J.
  2. Eur. J. Pharmacol. v.267 Characterstics of satbly expressed human dopamine $D_{1a} and D_{1b}$ receptors: Atypical behavior of the dopamine $D_{1b}$ receptors Paderson, U.B.;Norby, B.;Jensen, A.A,;Schiodt, M.;Hansen, A.;Suhr-Jessen, P.;Scheideler, M.;Thastrup, O.;Andersen, P.H.
  3. J. Biol. Chem. v.239 Tyrosine hydroxylase. The initial step in norepinephrine biosynthesis Nagatsu, T.;Levitt, M;Udenfriend, S.
  4. Arch. Pharm. Res. v.17 Inhibitory effects of the root of Coptis japonica on catecholamine biosynthesis in PC12 cells Lee, M.K.;Park, W.K.;Kim, H.S.
  5. Planta Med. v.62 Inhibitory effects of protoberberine alkaloids from the root of Coptis japonica on catecholamine buosynthesis in PC12 cells Lee, M.K.;Kim, H.S.
  6. Arch. Pharm. Res. v.20 Inhibitory effects of noscapine on dopamine biosynthesis in PC12 cells Shin, J.S.;Lee, S.S.;Lee, M.K.
  7. Med. Sci. Res. v.24 Inhibition of tyrosine hydroxylase by berberine Lee, M.K.;Zhang, Y.H.
  8. Arch. Pharm. Res. v.19 Inhibition of tyrosine hydroxylase by palmatine Lee, M.K.;Zhang, Y.H.;Kim, H.S.
  9. Med. Sci. Res. v.25 Inhibition of tyrosine hydroxylase by hydrastine Lee, M.K.;Zhang, Y.H.;Shin, J.S.;Lee, S.S.
  10. Planta Med. v.63 Inhibition of tyrosine hydroxylase by bulbocapnine Zhang, Y.H.;Shin, J.S.;Lee, S.S.;Kim, S.H.;Lee, M.K.
  11. J. Neurochem. v.30 Short-term regulation of catecholamine biosynthesis in a nerve growth factor responsive clonal line of ratpheochromocytoma cells Greene, L.A.;Rein, G.
  12. In Advance in Cellular Neurobiology v.3 PC12 pheo-chromatocytoma cultures in neurobiological research Greene, L.A.;Tischler, A.S.;Feroroff S.(Ed.)
  13. J. Chromatogr. v.344 High-performance liquid chromatography of plasma catecholamines using 1,2-fiphenylethylene-diamine as precolumn fluorescence derivatization reagent Mitsui, A.;Nohta, H.;Ohkura, Y.
  14. J. Chromatogr. v.378 Occurrence of aromatic L-amino acid decarboxylase in human plasma and its assay by high performance liquid chromatography with fluorescence detection Lee, M.K.;Nohta, H.;Ohkura, Y.
  15. J. Chromatogr. v.163 Highly sensitive assay for tyrosine hydroxylase activity by high-performance liquid chromatography Nagatsu, T.;Oka, K.;Kato, T.
  16. J. Biol. Chem. v.193 Protein measurement with the Folin phenol reagent Lowry, O.H.;Rosebrough, N.J.;Farr, A.L.;Randall, R.J.