The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effects of Artemisia Capillaris Fructus on Fas-FasL-induced Apoptosis in Hepatocye

인진호(茵蔯蒿)가 Fas-FasL 매개형 간세포 Apoptosis에 미치는 영향

  • Kim, Hyeong-Hwan (Dept. of Internal Medicine, College of oriental medicine, Dongguk University) ;
  • An, Joong-Hwan (Dept. of Internal Medicine, College of oriental medicine, Dongguk University) ;
  • Kim, Jong-Dae (Dept. of Internal Medicine, College of oriental medicine, Dongguk University) ;
  • Kim, Cheorl-Ho (Dept. of Biochemistry, College of oriental medicine, Dongguk University) ;
  • Kim, Seon-Kang (Dept. of Internal Medicine, College of oriental medicine, Dongguk University)
  • 김형환 (동국대학교 한의과대학 내과학교실) ;
  • 안중환 (동국대학교 한의과대학 내과학교실) ;
  • 김종대 (동국대학교 한의과대학 내과학교실) ;
  • 김철호 (동국대학교 한의과대학 생화학교실) ;
  • 김선강 (동국대학교 한의과대학 내과학교실)
  • Published : 2001.09.30
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Abstract

Objectives: Recently, it was known that the major cause of hepatitis is apoptosis reaction mediated by Fas-FasL. Since Artemisia Capillaris Fructus has long been applied to cure the jaundice in oriental medicine. Therefore, this study was carried out to examine the effect of fractions of Artemisia Capillaris Fructus on Fas-FasL-mediated apoptosis in hepatocytes. Methods: This study employed propidium iodide negative cell count assay and some the other biochemical assays. Results : This study confirms that hepatitis has been occured by apoptosis mediated by Fas-FasL in cultured hepatocyte and fractions of Artemisia Capillaris Fructus restrain apoptosis induced Fas-FasL. Conclusions : Water-extracted fraction, methanol extracts, ether-soluble fraction, and buthanol-soluble fractions of Artemisia Capillaris Fructus restrain Fas-FasL-mediated apoptosis in hepatocyte. Silica gel chromatograph of Buthanol-soluble fraction of Artemisia Capillaris Fructus restrain Fas-FasL-mediated apoptosis in hepatocyte. Artemisia Capillaris Fructus could be applied to cure hepatitis.

Keywords

References

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