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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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RU486 Suppresses Progesterone-induced Acrosome Reaction in Boar Spermatozoa

  • Jang, Sun-Phil (Department of Biological Science and the Institute for Basic Science, Sungkyunkwan University) ;
  • YiLee, S.H. (Department of Biological Science and the Institute for Basic Science, Sungkyunkwan University)
  • Published : 2002.11.30
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Abstract

The effects of progesterone on the acrosome reaction, as well as the effects of RU486 on the progesterone-induced acrosome reaction in capacitated boar spermatozoa, were investigated. Progesterone, a major steroid that is secreted by the cumulus cells of oocyte, clearly induced the acrosome reaction in a dose-dependent manner in capacitated boar spermatozoa, even though it failed to show similar effects in non-capacitated spermatozoa. RU486, a potent antiprogestin, significantly reduced the effects of progesterone on the progesterone-induced acrosome reaction; however, when treated alone, it showed no inhibitory effects on the acrosome reaction. The inhibitory effects of RU486 were also shown to be dose-dependent. These results imply that in addition to the well-known inducer of the acrosome reaction, zona pellucida, progesterone can also induce the acrosome reaction through its specific receptors on spermatozoa after the spermatozoa undergo capacitation.

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References

  1. Baldi, E., Casano, R., Falsetti, C., Krausz, C., Maggi, M. and Forti, G. (1991) Intracellular calcium accumulation and responsiveness to progesterone in capacitating human spermatozoa. J. Androl. 12, 323-330.
  2. Baldi, E., Luconi, M., Bonaccorsi, L. and Forti, G. (2002) Signal transduction pathways in human spermatozoa. J. Reprod. Immunol. 53, 121-131. Review. https://doi.org/10.1016/S0165-0378(01)00089-4
  3. Baulieu, E. E. (1989) Contragestion and other clinical applications of RU486, an antiprogesterone at the receptor. Science 245, 1351-1357. https://doi.org/10.1126/science.2781282
  4. Cross, N. L., Morales, P., Overstreet, J. M. and Hanson, F. W. (1988) Induction of acrosome reaction by the human zona pellucida. BioI. Reprod. 38, 235-244 https://doi.org/10.1095/biolreprod38.1.235
  5. Fazeli, A., Duncan, A. E., Watson, P. F. and Holt, W. V. (1999) Sperm-oviduct interaction: Induction of capacitation and preferential binding of uncapacitated spermatozoa to oviductal epithelial cells in porcine species. BioI. Reprod. 60, 879-886. https://doi.org/10.1095/biolreprod60.4.879
  6. Fraser, L. R. (1995) Cellular biology of capacitation and acrosome reaction. Hum. Reprod. Suppl. 10, 22-30.
  7. Fraser, L. R. and Herrod, J. E. (1990) Expression of capacitation-dependent changes in chlorotetracycline fluorescence pattern in mouse spermatozoa requires a suitable glycosylable substrate. J. Reprod. Fertil. 88, 611-621. https://doi.org/10.1530/jrf.0.0880611
  8. Meizel, S., Pillai, M. C., Diaz-Perez, E. and Thomas, P. (1990) Initiation of the human acrosome reaction by components of human follicular fluid and cumulus secretions including steroids. Serono Symposia. USA. 205-222.
  9. Meizel, S. and Turner, K. O. (1991) Progesterone acts at the plasma membrane of human sperm. Mol. Cell. Endocrinol. 11, R1-R5. https://doi.org/10.1016/0303-7207(78)90027-8
  10. Melendrez, C. S., Meizel, S. and Berger, T. (1994) Comparison of the ability of progesterone and heat solubilized porcine zona pellucida to initiate the porcine sperm acrosome reaction in vitro. Mol. Repmd. Dev. 39, 433-438. https://doi.org/10.1002/mrd.1080390412
  11. Meyers, S. A., Overstreet, J. W., Liu, I. K. M. and Drobnis, E. Z. (1995) Capacitation in vitro of stallion spermatozoa: comparison of progesterone-induced acrosome reactions in fertile and subfertile males. J. Androl. 16, 47-54.
  12. Osman, R. A., Andria, M. L., lones, A. D. and Meizel, S. (1989) Steroid induced exocytosis: the human sperm acrosome reaction. Biochem. Biophys. Res. Commun. 160, 828-833. https://doi.org/10.1016/0006-291X(89)92508-4
  13. Revelli. A., Massobori, M. and Tesarik, J. (1998) Nongenomic actions of steroid hormones in reproductive tissues. Endocr. Rev. 19. 3-17. https://doi.org/10.1210/er.19.1.3
  14. Sabuer, K., Edwards, D. P. and Meizel, S. (1996) Human sperm plasma membrane progesterone receptor(s) and the acrosome reaction. Biol. Reprod. 54, 993-1001. https://doi.org/10.1095/biolreprod54.5.993
  15. Somanuth, P. R ., Suraj, K. and Gandhi, K. K. (2000) Caprine sperm acrosome reaction: promotion by progesterone and homologous zona pellucida. Small Rumin. Res. 31, 279-286. https://doi.org/10.1016/S0921-4488(99)00148-0
  16. Suarez, S. S. and Dai, X. (1995) Intracellular calcium reaches different levels of elevation in hyperactivated and acrosome- reacted hamster sperm. Mol. Reprod. Dev. 42, 325-333. https://doi.org/10.1002/mrd.1080420310
  17. Tesarik, J., Carreras, A., and Mendoza, C. (1993) Differential sensitivity of progesterone and zona pellucida-induced acrosome reactions to pertussis toxin. Mol. Reprod. Dev. 34, 183-189. https://doi.org/10.1002/mrd.1080340210
  18. Tulsiani, D.R., Abou-Haila, A., Loeser, C. R. and Pereira, B. M. (1998) The biological and functional significance of the sperm acrosome and acrosomal enzymes in mammalian fertilization. Exp. Cell Res. 240, 151-164. https://doi.org/10.1006/excr.1998.3943
  19. Yamagata, K., Honda, A., Kashiwabara, S. I. and Baba, T. (1999) Difference of acrosomal serine protease system between mouse and other rodent sperm. Dev. Genet. 25, 115-122. https://doi.org/10.1002/(SICI)1520-6408(1999)25:2<115::AID-DVG5>3.0.CO;2-1
  20. Yanagimachi, R. (1994) Mammalian fertilization; in The Physiology Of Reproduction. Knobil. E and Neil, J. (eds), pp 135-186, Raven Press. New York.
  21. Yang, J., Serres, C., Phlibert, D., Robel, P. and Baulieu, E. E. (1994) Progesterone and RU486: Opposing effects on human sperm. Proc. Natl. Acad. Sci. USA 91. 529-533. https://doi.org/10.1073/pnas.91.2.529
  22. Yi, L. S. (1999) Amino acid composition analysis of the 32 kDa sperminogen. J. Biochem. Mol. Biol. 33, 510-513.
  23. Yu, H. and Vi, L. S. (2001) Purification and partial peptide sequence analysis of the boar 32 kDa sperminogen. Mol. Cells 31, 107-111.

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