The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지

Effects of Kangwhalyupung-tang on the Cerebral Neuronal Damage induced by Ischemia

강활유풍탕(羌活愈風湯)이 뇌허혈(腦虛血)로 유도된 대뇌신경세포손상(大腦神經細胞損傷)에 미치는 영향

  • Mun Beong-Sun (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Hwang Chung-Yeon (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Kim Keong-Yo (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee Geon-Mok (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Sung Kang-Keong (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee Dae-Yong (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University, Kunpo Hospital) ;
  • Lee Seoung-Geun (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University, Kunpo Hospital)
  • 문병순 (원광대학교 한의과대학) ;
  • 황충연 (원광대학교 한의과대학) ;
  • 김경요 (원광대학교 한의과대학) ;
  • 이건목 (원광대학교 한의과대학) ;
  • 성강경 (원광대학교 한의과대학) ;
  • 이대용 (원광대학교 군포한방병원 한방내과) ;
  • 이성근 (원광대학교 군포한방병원 한방내과)
  • Published : 2003.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Objective : Experimental studies have been done to elucidate the effect of kangwhalyupung-tang(KWYPT) on neuronal cell damage induced by brain ischemia. Method : The cytotoxic effect of ischemia was measured in the MTS assay cultures. MTS assay, INT assay, neurofilament(NF) enzymeimmuno assay(EIA). And the KWYPT on ischemia-induced neurotoxicity were examined by in vitro assays. Results : 1. The KWYPT protected effectively neuronal cell-death resulted from brain ischemia induced by the treatment of $95%N_2/5%CO_2$ for 10 min in those dependent fashion. 2. The KWYPT effectively increased the amount of NF resulting from brain ischemia, induced by the treatment of $95%N_2/5%CO_2$ for 15 min in those dependent fashions. Conclusions : KWYPT protects the brain ischemia-induced neurotoxicity through the increase of cell viability and of neurofilament in dose-dependent manner.

Keywords