Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Inhibitory Effect of Solvent Fraction of Various Kinds of Kimchi on Ultraviolet B Induced Oxidation and Erythema Formation of Hairless Mice Skin

김치종류별 용매 획분의 자외선 B 조사에 의해 유도된 피부산화 및 홍반 생성 억제 효과

  • 류복미 (부산대학교 식품영양학과 및 김치연구소) ;
  • 류승희 (인제대학교 바이오헬스 소재 연구센터, 식품과학연구소 및 식품생명과학) ;
  • 전영수 (부산대학교 식품영양학과 및 김치연구) ;
  • 이유순 (경북과학대학 향장공업) ;
  • 문갑순 (인제대학교 바이오헬스 소재 연구센터, 식품과학연구소 및 식품생명과학부)
  • Published : 2004.06.01
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Abstract

Kimchi is well known as a traditional Korean food containing various antioxidative compounds and it has been demonstrated that dietary kimchi inhibit the tissue oxidation and aging in many related studies. Ultraviolet B (UVB) radiation is the most potent among the many related factor to skin photo-aging. This experiment is designed to elucidate the inhibitory effect of solvent fractionation of various kimchi on UVB induced oxidation and erythema formation. Among solvent fractions, phenol content mostly existed in dichloromethane / ethylacetate fraction of buchu kimchi, dichloromethane fraction of Korean cabbage kimchi and ethylacetate / dichloromethane fraction of mustard leaf kimchi. Free radical scavenging activities were higher in dichloromethane and ethylacetate fraction from buchu kimchi, dichloromethane fraction from Korean cabbage kimchi, and ethylacetate fraction from mustard leaf kimchi. When the damage of photo-oxidation by UVB irradiation was tested in the presence of solvent fractions of kimchi, dichloromethane / ethylacetate fraction of buchu kimchi, ethylacetate fraction from Korean cabbage and from mustard leaf kimchi showed the higher protective activities than others. The erythema formations which were induced by UVB irradiation were decreased in solvent fractions of kimchi that hate higher antioxidant activities. In conclusion, kimchi solvent fractions having antioxidant compounds mostly inhibited photo-oxidation and erythema formation by UV-irradiation, therefore kimchi consumption can retard skin aging due to the presence of antioxidative compounds.

배추김치, 갓김치, 부추김치의 용매획분 추출물을 제조한 뒤 항산화관련 물질들의 함량을 측정한 결과 카로테노이드는 갓김치의 hexane층에서, 클로로필 함량은 부추김치의 hexane층에서 가장 높았고, 비타민 C함량은 갓김치의 EtOAc층과 BuOH층에서 가장 높게 나타났다. 총 폐놀화합물은 부추김 치의 CH$_2$Cl$_2$층과 EtOAc층, 배추김치 CH$_2$Cl$_2$층, 갖김치 EtOAc층과 CH$_2$Cl$_2$층에 많이 함유되어 있었다. DPPH법을 이용한 유리기 소거능을 조사한 결과 부추김치 CH$_2$Cl$_2$층과 EtOAc층, 배추김치 CH$_2$Cl$_2$층, 갓김치 EtOAc층에서 효과가 큰 것으로 나타났다. 김치종류별 용매획분을 hairless mouse의 피부균질액에 첨가하여 자외선 B를 조사한 후 지질과산 화물 함량을 측정한 결과 부추김치의 CH$_2$Cl$_2$층과 EtOAc층, 배추김치, 갓김치의 EtOAc층에서 낮게 나타나 항산화성이 높은 획분에서 피부의 광산화 억제효과가 큰 것을 알 수 있었고 김치 용매획분을 크림에 첨가하여 hairless mouse의 등에 직접 도포한 후 자외선 B를 조사하였을 때 대조군에 비해 갓김치의 hexane층, 부추김치의 hexane층과 BuOH층을 제외한 모든 용매획분이 피부홍반의 생성을 억제하였고 특히 갖김치 EtOAc층, 배추김치 CH$_2$Cl$_2$층, 부추김치 물층에서 피부보호효과가 큰 것으로 나타났다.

Keywords

References

  1. Cheigh HS. 1995. Biochemical characteristics of kimchi. J East Asian Soc Dietary Life 5: 89-101.
  2. Park KY. 1995. The nutritional evaluation, and antimutagenic and anticancer effects of kimchi. J Korean Soc Food Nutr 24: 169-182.
  3. Hwang JW, Song YO. 2000. The effects of solvent fractions of kimchi on plasma lipid concentration of rabbit fed high cholesterol diet. J Korean Soc Food Sci Nutr 29: 204-210.
  4. Jeon HN, Kwon MJ, Song YO. 2002. Effect of kimchi solvent fractions on accumulation of lipids in heart, kidney and lung of rabbit fed high cholesterol diet. J Korean Soc Food Sci Nurt 31: 814-818. https://doi.org/10.3746/jkfn.2002.31.5.814
  5. Lee YO, Cheigh HS. 1995. Antioxidative effect of kimchi on the lipid oxidation of cooked meat. J Korean Soc Food Nutr 24: 1005-1009.
  6. Lee YO, Park KY, Cheigh HS. 1996. Antioxidative effect of kimchi with various fermentation period on the lipid oxidation of cooked ground meat. J Korean Soc Food Nutr 25: 261-266.
  7. Ryu SH, Kwon MJ, Song YO, Song YS, Moon GS. 2003. Effect of kimchi diet on antioxidative system of rat. Inje J Inje Univ (Korea) 18: 475-488.
  8. Kim JH, Kwon MJ, Lee SY, Ryu JD, Moon GS, Cheigh HS, Song YS. 2002. The effect of kimchi intake on production of free radicals and anti-oxidative enzyme activities in the liver of SAM. J Korean Soc Food Sci Nutr 31: 109-116. https://doi.org/10.3746/jkfn.2002.31.1.109
  9. Kim JH, Ryu JD, Lee HG, Park JH, Moon GS, Cheigh HS, Song YS. 2002. The effect of kimchi intake on production of free radicals and anti-oxidative enzyme activities in the brain of SAM. J Korean Soc Food Sci Nutr 31: 117-123. https://doi.org/10.3746/jkfn.2002.31.1.117
  10. Ryu BM, Ryu SH, Lee YS, Jeon YS, Moon GS. 2004. Effect of different kimchi diets on oxidation and photooxidation in hairless mouse. J Korean Soc Food Sci Nutr 33: 291-298. https://doi.org/10.3746/jkfn.2004.33.2.291
  11. Danno K, Horio T, Takigawa M, Imamura S. 1984. Role of oxygen intermediates in UV-induced epidermal cell injury. J Invest Dermatol 83: 166-168. https://doi.org/10.1111/1523-1747.ep12263506
  12. Miyachi Y, Horio T, Imamura S. 1983. Sunburn cell formation is prevented by scavenging oxygen intermediates. Clinical and Experimental Dermatology 8: 305-310. https://doi.org/10.1111/j.1365-2230.1983.tb01784.x
  13. Choi JY, Yoon KS, Cho YH, Kim NI. 1996. The effect of UVB irradiation and sunscreen agents on photoaging of hairless albino mouse skin. Kor J Dermatol 34: 289-299.
  14. Iwasaki TK, Sakamoto K, Packer L. 1997. Interaction between vitamin E homologues and ascorbate free radicals in murine skin homogenates irradiated with ultraviolet light. Photochem & Photobiol 65: 355-365. https://doi.org/10.1111/j.1751-1097.1997.tb08571.x
  15. Ryu SH, Jeon YS, Kwon MJ, Moon JW, Lee YS, Moon GS. 1997. Effect of kimchi extracts to reactive oxygen species in skin cell cytotoxicity. J Korean Soc Food Sci Nutr 26: 814-821.
  16. Blois MS. 1958. Antioxidant determinations by the use of stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  17. Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95: 351-358. https://doi.org/10.1016/0003-2697(79)90738-3
  18. Gonalez S, Pathak MA. 1996. Inhibition of ultravioletinduced formation of reactive oxygen species, lipid peroxidation, erythema and skin photosensitization by polypodium leucotomes. Photodermatol Photoimmunol Photomed 12: 45-56. https://doi.org/10.1111/j.1600-0781.1996.tb00175.x
  19. Kim JI. 2000. Studies on antioxidative flavonoids and nucleotide from mustard leaf kimchi. PhD Thesis. Pusan National University, Busan, Korea.
  20. Usuki R, Endo Y, Kaneda T. 1984. Prooxidant activities of chlorophylls and pheophytins on the photooxidation of edible oils. Agric Biol Chem 48: 991-994. https://doi.org/10.1271/bbb1961.48.991
  21. McAuliffe DJ, Blank IH. 1991. Effects of UVA on the barrier characteristics of the skin. J Invest Dermatol 96: 758-762. https://doi.org/10.1111/1523-1747.ep12471711
  22. Kim BK, Park SB, Youn JI. 1997. Relationship between UV-induced erythema and pigmentation dose-response angles and the predictors of cutaneous sensitivity to UV right. Kor J Dermatol 35: 1177-1183.

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