Transactions of the Korean Society of Mechanical Engineers A (대한기계학회논문집A)

The Korean Society of Mechanical Engineers (대한기계학회)
권호 표지
DOI QR코드

DOI QR Code

Microfilter Chip Fabrication for Bead-Based Immunoassay

비드를 이용한 면역분석용 마이크로필터 칩의 제작

  • 이승우 (한양대학교 대학원 정밀기계공학과) ;
  • 안유민 (한양대학교 기계공학과, 마이크로바이오칩센터) ;
  • 채영규 (한양대학교 생화학및분자생물, 마이크로바이오칩센터)
  • Published : 2004.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Immunoassay is one of the important analytical methods for clinical diagnoses and biochemical studies, but needs a long time, troublesome procedures and expensive reagents. In this study, therefore, we propose the micro filter chip with microbeads for immunoassay, which has pillar structures. The advantage of the proposed micro filter chip is to use simple fabrication process and cheap materials. The mold was made by the photolithography technique with Si wafer and negative photoresist SU-8. The replica was made of PDMS, bonded on the pyrex glass. The micro filter chip consists of inlet channel, filter chamber and outlet channel. HBV (Hepatitius B virus) monoclonal antibody (Ag1) labeled with biotin were immobilized onto streptavidin coated beads of 30∼50 $\mu$m size. Fluorescein isothiocyanate (FITC)-labeled HBV monoclonal antibody (Ag8) was used to detect HBsAg (Hebatitis B virus surface Antigen), and fluorescence intensity was monitored by epi-fluorescence microscope. In this study, the immune response of less than 30 min was obtained with with the use of 100 $m\ell$ of sample.

Keywords

References

  1. Kim, J. K., Chung, C, Chang, J. K. and Yoo, J. Y, 2003, 'Analysis of Fully Developed Mulitilayer Flow in MicroChannel with a Rectangular Cross Section,' Trans, of the KSME (B), Vol. 27, No. 5, pp. 644-654
  2. Lee, D., Maeng, J.-S, Choi, H.-I. and Na, W., 2003, 'Predictions of Microscale Separated Flow using Langmuir Slip Boundary Condition,' Trans. of the KSME (B), Vol. 27, No. 8, pp. 1097-1104
  3. Manz, A. and Becker, H. Eds., 1999, Microsystem Technology in Chemistry and Life Sciences, Springer-Verlag, Berlin Heidelberg, p. 10
  4. Hatch, A., Kamholz, A. E., Hawkins, K. R., Munson, M. S., Schilling, E. A., Weigl, B. H. and Yager, P., 2001, 'A Rapid Diffusion Immunoassay in a T-sensor,' Nat. Biotechnol., Vol. 19, pp. 461-465 https://doi.org/10.1038/88135
  5. Sato, K., Tokeshi, M., Kimura, H. and Kitamori, T., 2001, 'Determination of Carcinoembryonic Antigen in Human Sera by Integrated Bead-Bed Immunoasay in a Microchip for Cancer Diagnosis,' Anal. Chetn., Vol. 73, pp. 1213-1218 https://doi.org/10.1021/ac000991z
  6. Andersson, H., Van der Wijngaart, W., Enoksson, P., Stemme, G, 2000, 'Micromachined Flow-through Filter-Chamber for Chemical Reactions on Beads,' Sens. Actuators B, Vol. 67, pp. 203-208 https://doi.org/10.1016/S0925-4005(00)00413-5
  7. Seong, G H. and Crook, R. M., 2002, 'Efficient Mixing and Reactions within Microfluidic Channels Using Microbead - Supported Catalysts,' J. Am. Chem. Soc. Vol. 124, pp. 13360-13361 https://doi.org/10.1021/ja020932y
  8. Armani, D., Liu, C, Aluru, N., 1999, 'Re-configurable Fluid Circuits by PDMS Elastomer Micro-maching,' Proceedings of IEEE MEMS, IEEE, Washington DC, pp. 222-227