Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Hypoglycemic Properties of Polysaccharides Extracted from Ganoderma lucidum in Alloxan-Induced Diabetic Rats

  • Gao, Yihuai (Institute of Food, Nutrition and Human Health, Massey University) ;
  • Gao, He (New Zealand Institute of Natural Medicine, Auckland, New Zealand) ;
  • Chan, Eli (Department of Pharmacy, Faculty of Science, National University of Singapore) ;
  • Tan, Wenbo (New Zealand Institute of Natural Medicine, Auckland, New Zealand) ;
  • Lan, Jin (New Zealand Institute of Natural Medicine, Auckland, New Zealand) ;
  • Koh, Hwee-Ling (Department of Pharmacy, Faculty of Science, National University of Singapore) ;
  • Chen, Guoliang (New Zealand Institute of Natural Medicine, Auckland, New Zealand) ;
  • Zhou, Shufeng (Department of Pharmacy, Faculty of Science, National University of Singapore)
  • Published : 2004.09.01
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Abstract

A recent randomized and double-blind placebo-controlled clinical study bas indicated that Ganoderma lucidum polysaccharides (GLP) decrease blood glucose in patients with type II diabetes. The aim of this study was to investigate the effect of the GLP extract in alloxan-induced diabetic rats. Oral administration of GLP at 0.25, 0.5 and 1.0 g/kg for 4 weeks resulted in a reduction of blood glucose levels by 12.5, 18.7 and 33.7% respectively, while glibenclamide treatment brought the hyperglycemic value down to normal. The hyperglycemic effect was supported by a significant decrease in glycosylated haemoglobin and increased plasma insulin levels (p<0.01) in a dose- and time-dependent manner. This study showed that GLP has similar hypoglycemic effects as glibenclamide in alloxan-induced diabetic rats.

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References

  1. Andersson DKG, Svardsudd K. 1995. Long term glycemic control related to mortality in type II diabetes. Diabetes Care 18: 1534-1543 https://doi.org/10.2337/diacare.18.12.1534
  2. Ohkubo Y, Kishikawa H, Araki E, Miyata T, Isami S, Motoyoshi S, Kojima Y, Furuyoshi N, Shichiri M. 1995. Intensive insulin therapy prevents the progression of diabetic microvascular complications in Japanese patients with non-insulin-dependent diabetes mellitus: A randomized prospective 6 year study. Diabetes Res Clin Pract 28: 103- 117 https://doi.org/10.1016/0168-8227(95)01064-K
  3. Pugliese A, Miceli D. 2002. The insulin gene in diabetes. Diabetes Metab Res Rev 18: 13-25 https://doi.org/10.1002/dmrr.261
  4. Skyler JS. 1996. Diabetic complications: the importance of glucose control. Endocrinol Metab Clin North Am 25: 243-254 https://doi.org/10.1016/S0889-8529(05)70323-6
  5. Groop LC. 1996. Drug treatment of NIDDM. In Textbook of Diabetes. Pickup JC, Williams G, eds. Blackwell, Oxford. p 1-17
  6. Yap WS, Peterson GM, Vial JH, Randall CTC, Greenaway TM. 1998. Review of management of type 2 diabetes mellitus. J Clin Pharm Ther 23: 457-465 https://doi.org/10.1046/j.1365-2710.1998.00185.x
  7. Hsia SH. 2001. Modern pharmacotherapies for type 2 diabetes mellitus. J Nat'l Med Assoc 93: 335-349
  8. Gao YH, Lan J, Dai XH, Ye JX, Zhou SF. 2004. A Phase I/II study of Ling Zhi mushroom Ganoderma lucidum (W. Curt.:Fr.) Lloyd (Aphyllophoromycetideae) extract in patients with type II diabetes mellitus. Int J Med Mushr 6: 33-39 https://doi.org/10.1615/IntJMedMushr.v6.i1.30
  9. Shiao MS, Lee KR, Lin LJ, Wang CT. 1994. Natural products and biological activities of the Chinese medical fungus, Ganoderma lucidum. In Food phytochemicals for cancer prevention. II: Teas, spices, and herbs. Ho CT, Osawa T, Huang MT, Rosen RT, eds. American Chemical Society, Washington DC. p 342-354
  10. Wasser SP, Weis AL. 1999. Therapeutic effects of substances occurring in higher basidiomycetes mushrooms: A modern perspective. Crit Rev Immunol 19: 65-96
  11. Gao YH, Zhou SF. 2003. Cancer prevention and treatment by Ganoderma, a mushroom with medicinal properties. Food Rev Int 19: 275-325 https://doi.org/10.1081/FRI-120023480
  12. Gao YH, Zhou SF, Chen GL, Lan J, Ye JX. 2003. Effect of Ganopoly, a Ganoderma lucidum polysaccharide extract on the immunological function in advanced-stage cancer patients. Immunol Invest 32: 201-215 https://doi.org/10.1081/IMM-120022979
  13. Gao YH, Zhou SF, Huang M, Lan J, Gao H. 2002. Mechanisms for the protective effects of Ganoderma lucidum polysaccharide fraction on indomethacin-induced ulcer in the rat. Life Sci 72: 731-745 https://doi.org/10.1016/S0024-3205(02)02301-9
  14. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determination of sugar and related substances. Anal Chem 28: 350-356 https://doi.org/10.1021/ac60111a017
  15. Alsop RM, Vlachogiannis GJ. 1982. Determination of the molecular weight of clinical dextrans by gel permeation chromatography on TSK PW type columns. J Chromatogr 246: 227-240 https://doi.org/10.1016/S0021-9673(00)95862-X
  16. Dische Z. 1947. A new specific color reaction of hexuronic acids. J Biol Chem 167: 189-198
  17. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Garter FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC. 1985. Measurement of protein using bicinchoninic acid. Anal Biochem 150: 76-85
  18. Lindsay GK, Roslansky PF, Novitsky TJ. 1989. Single- step, chromogenic Limulus amebocyte lysate assay for endotoxin. J Clin Microbiol 27: 947-951
  19. Roth RI, Levin FC, Levin J. 1990. Optimization of detection of bacterial endotoxin in plasma with the Limulus test. J Lab Clin Med 116: 153-161
  20. Sasaki T, Matsy S, Sonae A. 1972. Effect of acetic acid concentration on the colour reaction in the o-toluidine-boric acid method for glucose estimation. Rinsho Kagaku 1: 346-353
  21. Drabkin DL, Austin JM. 1932. Spectrophotometric studies, spectrophotometric constants for common haemoglobin derivatives in human, dog and rabbit blood. J Biol Chem 98: 719-733
  22. Nayak SS, Pattabiraman TN. 1981. A new colorimetric method for the estimation of glycosylated haemoglobin. Clin Chim Acta 109: 267-274 https://doi.org/10.1016/0009-8981(81)90312-0
  23. Zhang HN, He JH, Yuan L, Lin ZB. 2003. In vitro and in vivo protective effect of Ganoderma lucidum polysaccharides on alloxan-induced pancreatic islets damage. Life Sci 73: 2307-2319 https://doi.org/10.1016/S0024-3205(03)00594-0
  24. Vickers A. 2000. Recent advances: Complementary medicine. Br Med J 321: 683-686 https://doi.org/10.1136/bmj.321.7262.683
  25. Petlevski R, Hadzija M, Slijepcevic M, Juretic D. 2001. Effect of 'antidiabetis' herbal preparation on serum glucose and fructosamine in NOD mice. J Ethnopharmacol 75: 181-184 https://doi.org/10.1016/S0378-8741(01)00177-5
  26. Grover JK, Yadav S, Vats V. 2002. Medicinal plants of India with anti-diabetic potential. J Ethnopharmacol 81: 81-100 https://doi.org/10.1016/S0378-8741(02)00059-4
  27. Murali B, Upadhyaya UM, Goyal RK. 2002. Effect of chronic treatment with Enicostemma littorale innon-insulin- dependent diabetic (NIDDM) rats. J Ethnopharmacol 81: 199-204 https://doi.org/10.1016/S0378-8741(02)00077-6
  28. Yang BK, Kim DH, Jeong SC, Das S, Choi YS, Shin JS, Lee SC, Song CH. 2002. Hypoglycemic effect of a Lentinus edodes exo-polymer produced from a submerged mycelial culture. Biosci Biotech Biochem 66: 937-942 https://doi.org/10.1271/bbb.66.937
  29. Pari L, Ramakrishnan R, Venkateswaran S. 2001. Antihyperglycaemic effect of Diamed, a herbal formulation, in experimental diabetes in rats. J Pharm Pharmacol 53: 1139-1143 https://doi.org/10.1211/0022357011776397
  30. Dunn D, Sheehan H, McLetchin N. 1943. Necrosis of islets of Langherhans produced experimentally. Lancet 244: 484- 494 https://doi.org/10.1016/S0140-6736(00)45317-1
  31. Lenzen S, Munday R. 1991. Thiol group reactivity, hydrophilicity and stability of alloxan, its reduction products and its N-methyl derivatives and a comparison with ninhydrin. Biochem Pharmacol 42: 1385-1391 https://doi.org/10.1016/0006-2952(91)90449-F
  32. Muller A, Rice PJ, Ensley H, Coogan PS, Kalbfleisch JH, Kelley JL, Love EJ, Portera CA, Ha TZ, Browder IW, Williams DL. 1996. Receptor binding and internalization of a water-soluble (1$\rightarrow$3)-beta-D-glucan biologic response modifier in two monocyte macrophage cell lines. J Immunol 156: 3418-3425
  33. Muller A, Raptis J, Rice PJ, Kalbfleisch JH, Stout RD, Ensley HE, Browder W, Williams DL. 2000. The influence of glucan polymer structure and solution conformation on binding to (1$\rightarrow$3)-beta-D-glucan receptors in a human monocyte-like cell line. Glycobiology 10: 339-346 https://doi.org/10.1093/glycob/10.4.339
  34. Kumar GP, Sudheesh S, Vijayalakshmi NR. 1993. Hypoglycemic effect of Coccinia indica: Mechanism of action. Planta Med 59: 330-332 https://doi.org/10.1055/s-2006-959693

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