Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Effect of Bisphenol A on Ovarian Steroidogenesis in Longchin Goby (Chasmichthys dolichognathus)

Bisphenol A가 점 망둑 (Chasmichthys dolichognothus)의 난소 스테로이드 호르몬 대사에 미치는 영향

  • BAEK Hea-Ja (Department of Marine Biology, Pukyong National University) ;
  • PARK Myoung-Hee (Department of Marine Biology, Pukyong National University) ;
  • LEE Young-Don (Marine and Environmental Research Institute, Cheju National University) ;
  • KIM Hyung-Bae (Department of Marine Bio-resources, Gangwon Provincial University) ;
  • KIM Jae-Won (Department of Marine Biology, Pukyong National University) ;
  • YOO Myoung-Suk (Department of Marine Biology, Pukyong National University)
  • 백혜자 (부경대학교 자원생물학과) ;
  • 박명희 (부경대학교 자원생물학과) ;
  • 이영돈 (제주대학교 해양과환경연구소) ;
  • 김형배 (강원도립대학 해양생물자원개발과) ;
  • 김재원 (부경대학교 자원생물학과) ;
  • 유명숙 (부경대학교 자원생물학과)
  • Published : 2004.06.01
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Abstract

The in vitro effect of bisphenol A (BPA) on ovarian steroidogenesis of the longchin goby (Chasmichthys dolichognathus) was investigated. Oocytes taken during the maturing phase (vitellogenic, fully vitellogenic or germinal vesicle breakdown stage) were incubated with BPA (100 ng/mL) in the presence of exogenous precursor $^{3}H-17\alpha\;hydroxyprogesterone\;(^{3}H-17\alphaOHP).$ Steroids were extracted from the media and the isolated oocytes, and the extracts were separated and identified by thin layer chromatography and gas chromatography-mass spectrometry. The identities of the major metabolites were progestogens $[17{\alpha}-hydroxy,20{\alpha}-dihydroprogesterone\;(17{\alpha}20{\alpha}OHP)\;and\;17{\alpha}-hydrxy,20{\beta}-dihydroprogesterone\;(17{\alpha}20{\beta}OHP),$ androgens [androstenedione (A4) and testosterone (T)] and estrogens [estrone $(E_1)\;and\;estradiol-17{\beta}(E_2)].$ BPA treatment inhibited production of estrogens in all the maturing phases and progestogens in the germinal vesicle migrating stage. Percentage yield of estrogens was decreased with increased yield of androgens. In conclusion, BPA had an inhibitory effect on the conversion of $^3H-17\alphaOHP$ to estrogens and progestogens. These results demonstrate that BPA can act either estrogenic or anti-estrogenic effects.

Keywords

References

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