Abstract
Free amino acids and polysaccharide content of submerged mycelial culture of Fomitopsis pinicola using $2\%$ citrus peel water extracts(CP), $2\%$ green tea water extracts(GT) and yeast-malt broth(YM) were investigated. Cultivations were conducted at $30^{\circ}C$ and 150 rpm for 10 days. Yields of the mycelium as fresh weight basis in GT-, CP- and YM-broth were $42.3\%,\;34.2\%\;and\;9.89\%$, and their turbidity(OD at 660 nm) of the broth without mycelium were 0.14, 0.16 and 0.22, respectively. Total free amino acid content in the mycelium were 928.19 $mg\%$ in YM, 1060.53 $mg\%$ in CP, 764.83 $mg\%$ in GT, and the major free amino acid was lysine in YM, glutamic acid in CP and GT. Total free exo-amino acid contents were 659.75 $mg\%$ in YM, 954.55 $mg\%$ in CP, 838.69 $mg\%$ in GT, and the major in the all broths was glutamic acid Total amino acid derivatives content of the mycelium were in order of CP>GT>YM, and the major was cystathionine in YM, hydroxy proline in CP and GT. The major among exo-amino derivatives was hydroxy proline in the all broth. Exo-AIS content was in order of CP>GT>YM. Acid soluble polysaccharide content of the mycelium was GT($0.69\%$)>YM($0.39\%$)>CP($0.18\%$). The exo-polysaccharide content was in order of GT($0.87\%$)>CP($0.69\%$)>YM($0.09\%$). Alkali soluble polysaccharide content of the mycelium was in order of CP($5.21\%$)>GT($5.18\%$)>YM($4.56\%$), and exo-polysaccharide was in order of GT($6.79\%$)>YM($3.57\%$)>CP($3.01\%$). The alkali soluble polysaccharide eluted from mycelium cultivated in CP broth was supposed to polysaccharide(about 500,000 daltons) composed of hexose and uronic acid bounded with protein(below 10,000 daltons).
천연배지에서 배양한 소나무잔나비버섯(Fomitopsis pinicola)균사체와 균체외 유리아미노산 및 다당류함량을 조사하였다. 배지로는 YM(yeast-malt broth), CP(citrus peel-broth) 및 GT 배지(green tea-broth)를 사용하였으며 $30^{\circ}C$, 150rpm에서 10일간 배양하였다. 균사체의 수율은 GT, CP 및 YM에서 각각 42.3, 34.2 및 $9.89\%$였으며, 균사체를 제거한 배양액의 탁도는 GT 0.14, CP 0.16, YM 0.22이었다. 균사체의 총 유리아미노산 함량은 YM 928.19 $mg\%$, CP 1060.53 $mg\%$, GT 764.83 $mg\%$였으며, 주 아미노산은 YM은 lysine, CP와 GT는 glutamic acid였다. 균체외 총 유리아미노산 함량은 YM 659.75 $mg\%$, CP 954.55 $mg\%$, GT 838.69 $mg\%$였으며, 주 아미노산은 YM, CP 및 GT 모두 glutamic acid였다. 균사체와 균체외 total 아미노산 유도체의 함량은 CP>GT>YM 순이었다. 균사체의 주 아미노산 유도체는 YM은 cystathionine, CP와 GT는 hydroxy proline이였다. YM, CP 및 GT의 균체외 주 아미노산 유도체는 hydroxy proline이였다. 균사체와 균체외 AIS 함량은 CP>GT>YM순이었다. 산가용성 다당류 함량은 GT($0.69\%$)>YM($0.39\%$)>CP($0.18\%$)순, 균체외 산가용성 다당류 함량은 GT($0.87\%$)>CP($0.69\%$)>YM($0.09\%$)순이었다. 균사체의 알칼리 가용성 다당류 함량은 CP($5.21\%$)>GT($5.18\%$)>YM($4.56\%$) 순, 균체외는 GT($6.79\%$)>YM($3.57\%$)>CP($3.01\%$)순으로, 균사체 함유 다당류는 산가용성($0.18{\sim}0.69\%$)에 비하여 알칼리가용성($4.56{\sim}5.21\%$)의 함량이 현저하게 높았다. CP 배지에서 배양한 균사체의 알칼리가용성 다당류를 겔 여과한 결과 hexose와 uronic acid로 구성된 분자량 500,000 dalton의 다당류와 분자량 10,000 dalton이하의 단백질이 결합한 단백 다당체로 추정되었다.
