DOI QR코드

DOI QR Code

Two-component Signal Transduction in Synechocystis sp. PCC 6803 under Phosphate Limitation: Role of Acetyl Phosphate

  • Juntarajumnong, Waraporn (Department of Biochemistry, Faculty of Science, Chulalongkorn University) ;
  • Eaton-Rye, Julian J. (Department of Biochemistry, University of Otago) ;
  • Incharoensakdi, Aran (Department of Biochemistry, Faculty of Science, Chulalongkorn University)
  • 발행 : 2007.09.30

초록

The two-component signal transduction, which typically consists of a histidine kinase and a response regulator, is used by bacterial cells to sense changes in their environment. Previously, the SphS-SphR histidine kinase and response regulator pair of phosphate sensing signal transduction has been identified in Synechocystis sp. PCC 6803. In addition, some response regulators in bacteria have been shown to be cross regulated by low molecular weight phosphorylated compounds in the absence of the cognate histidine kinase. The ability of an endogenous acetyl phosphate to phosphorylate the response regulator, SphR in the absence of the cognate histidine kinase, SphS was therefore tested in Synechocystis sp. PCC 6803. The mutant lacking functional SphS and acetate kinase showed no detectable alkaline phosphatase activity under phosphate-limiting growth conditions. The results suggested that the endogenous acetyl phosphate accumulated inside the mutants could not activate the SphR via phosphorylation. On the other hand, exogenous acetyl phosphate could allow the mutant lacking functional acetate kinase and phosphotransacetylase to grow under phosphate-limiting conditions suggesting the role of acetyl phosphate as an energy source. Reverse transcription PCR demonstrated that the transcripts of acetate kinase and phospho-transacetylase genes in Synechocystis sp. PCC 6803 is up-regulated in response to phosphate limitation suggesting the importance of these two enzymes for energy metabolism in Synechocystis cells

참고문헌

  1. Amemura, M., Makino, K., Shinagawa, H. and Nakata, A. (1990) Cross talk to the phosphate regulon of Escherichia coli by PhoM protein: PhoM is a histidine protein kinase and catalyzes phosphorylation of PhoB and PhoM-open reading frame 2. J. Bacteriol. 172, 6300-6307. https://doi.org/10.1128/jb.172.11.6300-6307.1990
  2. Eaton-Rye, J. J. (2004) The construction of gene knockouts in the cyanobacterium Synechocystis sp. PCC 6803; in Photosysthesis Research Protocols, Carpentier, R. (ed.), pp. 309-324, Humana Press, Totowa, USA.
  3. Hirani, T. A. (2001) Identification and characterization of a twocomponent system involved in phosphate sensing in Synechocystis sp. PCC 6803. Ph. D. thesis. University of Otago, New Zealand.
  4. Hirani, T. A., Suzuki, I., Murata, N., Hayashi, H. and Eaton-Rye, J. J. (2000) Characterization of a two-component signal transduction system involved in the induction of alkaline phosphatase under phosphate-limiting conditions in Synechocystis sp. PCC 6803. Plant Mol. Biol. 45, 133-144. https://doi.org/10.1023/A:1006425214168
  5. Hiratsu, K., Nakata, A., Shinagawa, H. and Makino, K. (1995) Autophosphorylation and activation of transcriptional activator PhoB of Escherichia coli by acetyl phosphate in vitro. Gene 161, 7-10. https://doi.org/10.1016/0378-1119(95)00259-9
  6. Hsing, W., Russo, F. D., Bernd, K. K. and Silhavy, T. J. (1998) Mutations that alter the kinase and phosphatase activities of the two-component sensor EnvZ. J. Bacteriol. 180, 4583-4586.
  7. Kakuda, H., Hosono, K., Shiroishi, K. and Ichihara, S. (1994) Identification and characterization of the ackA (acetate kinase A)-pta (phosphotransacetylase) operon and complementation analysis of acetate utilization by an ackA-pta deletion mutant of Escherichia coli. J. Biochem. 116, 916-922. https://doi.org/10.1093/oxfordjournals.jbchem.a124616
  8. Lee, T., Makino, K., Shinagawa, H. and Nakata, A. (1990) Overproduction of acetate kinase activates the phosphate regulon in the absence of the phoR and phoM functions in Escherichia coli. J. Bacteriol. 172, 2245-2249. https://doi.org/10.1128/jb.172.5.2245-2249.1990
  9. McCleary, W. R. and Stock, J. B. (1994) Acetyl phosphate and the activation of two-component response regulators. J. Biol. Chem. 269, 31467-31572.
  10. McCleary, W. R., Stock, J. B. and Ninfa, A. J. (1993) Is acetyl phosphate a global signal in Escherichia coli? J. Bacteriol. 175, 2793-2798. https://doi.org/10.1128/jb.175.10.2793-2798.1993
  11. Morgan, T. R., Shand, J. A., Clarke, S. M. and Eaton-Rye, J. J. (1998) Specific requirements for cytochrome c-550 and the manganese-stabilizing protein in photoautotrophic strains of Synechocystis sp. PCC 6803 with mutations in the domain Gly-351 to Thr-436 of the chlorophyll-binding protein CP47. Biochemistry 37, 14437-14449. https://doi.org/10.1021/bi980404s
  12. Morrison, S. S., Mullineaux, C. W. and Ashby, M. K. (2005) The influence of acetyl phosphate on DspA signaling in the cyanobacterium Synechocystis sp. PCC6803. BMC Microbiol. 5, 47. https://doi.org/10.1186/1471-2180-5-47
  13. Murata, N. and Suzuki, I. (2006) Exploitation of genomic sequences in a systematic analysis to assess how cyanobacteria sense environmental stress. J. Exp. Bot. 57, 235-247. https://doi.org/10.1093/jxb/erj005
  14. Parkinson, J. S. and Kofoid, E. C. (1992) Communication modules in bacterial signaling proteins. Ann. Rev. Genet. 26, 71-112. https://doi.org/10.1146/annurev.ge.26.120192.000443
  15. Singh-Wissmann, K. and Ferry, J. G. (1995) Transcriptional regulation of the phosphotransacetylase-encoding and acetate kinase-encoding genes (pta and ack) from Methanosarcina thermophila. J. Bacteriol. 177, 1699-1702. https://doi.org/10.1128/jb.177.7.1699-1702.1995
  16. Stock, J. B., Surette, M. G., Levitt, M. and Park, P. (1995) Two-component signal transduction systems: structure-function relationships and mechanisms of catalysis. ; in Two-component Signal Transduction, Hoch, J. A. and Silhavy, T. J. (eds.), pp 25-51, American Society for Microbiology, Washington, USA.
  17. Summers, M. L., Denton, M. C. and McDermott, T. R. (1999) Genes coding for phosphotransacetylase and acetate kinase in Sinorhizobium meliloti are in an operon that is inducible by phosphate stress and controlled by PhoB. J. Bacteriol. 181, 2217-2224.
  18. Suzuki, S., Ferjan, A., Suzuk,i I. and Murata, N. (2004) The SphS-SphR two component system is the exclusive sensor for the induction of gene expression in response to phosphate limitation in Synechocystis. J. Biol. Chem. 279, 13234-13240. https://doi.org/10.1074/jbc.M313358200
  19. Wanner, B. L. (1995) Signal transduction and cross regulation in the Escherichia coli phosphate regulon by PhoR, CreC, and acetyl phosphate in Two-component Signal Transduction, Hoch, J. A. and Silhavy, T. J. (ed), pp. 203-221, American Society for Microbiology, Washington, USA.
  20. Wanner, B. L. and Wilmes-riesenberg, M. R. (1992) Involvement of phosphotransacetylase, acetate kinase, and acetyl phosphate synthesis in control of the phosphate regulon in Escherichia coli. J. Bacteriol. 174, 2124-2130. https://doi.org/10.1128/jb.174.7.2124-2130.1992
  21. Williams, J. G. K. (1988) Construction of specific mutations in the photosystem II photosynthetic reaction center by genetic engineering methods in the cyanobacterium Synechocystis sp. PCC 6803. Methods Enzymol. 167, 776-778.
  22. Wolfe, A. J. (2005) The acetate switch. Micro. Mol. Biol. Rev. 69, 12-50. https://doi.org/10.1128/MMBR.69.1.12-50.2005

피인용 문헌

  1. Production of optically pure d-lactate from CO2 by blocking the PHB and acetate pathways and expressing d-lactate dehydrogenase in cyanobacterium Synechocystis sp. PCC 6803 vol.49, pp.12, 2014, https://doi.org/10.1016/j.procbio.2014.09.007
  2. Phosphorus removal in a closed recirculating aquaculture system using the cyanobacterium Synechocystis sp. PCC 6803 strain lacking the SphU regulator of the Pho regulon vol.74, 2013, https://doi.org/10.1016/j.bej.2013.03.004
  3. Essential role of the plasmidhik31operon in regulating central metabolism in the dark inSynechocystissp. PCC 6803 vol.91, pp.1, 2014, https://doi.org/10.1111/mmi.12442
  4. The extended N-terminal region of SphS is required for detection of external phosphate levels in Synechocystis sp. PCC 6803 vol.378, pp.3, 2009, https://doi.org/10.1016/j.bbrc.2008.11.012
  5. Stress Sensors and Signal Transducers in Cyanobacteria vol.10, pp.3, 2010, https://doi.org/10.3390/s100302386
  6. Designing and creating a modularized synthetic pathway in cyanobacterium Synechocystis enables production of acetone from carbon dioxide vol.14, pp.4, 2012, https://doi.org/10.1016/j.ymben.2012.03.005
  7. Powerful fermentative hydrogen evolution of photosynthate in the cyanobacterium Lyngbya aestuarii BL J mediated by a bidirectional hydrogenase vol.5, 2014, https://doi.org/10.3389/fmicb.2014.00680