Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Plant Regeneration via Organogenesis from Leaf Explant Culture of Taraxacum coreanum Nakai

흰민들레 잎조직으로 부터 기관형성을 통한 식물체 재생

  • Lee, Hyun-Hwa (Department of Biology, Collage of Nature Science, Chosun University) ;
  • Kim, Young-Soon (Kumho Life and Environmental Science Laboratory, Chonnam National University) ;
  • Park, Hyeon-Yong (Department of Biology, Collage of Nature Science, Chosun University)
  • 이현화 (조선대학교 자연과학대학 생물학과) ;
  • 김영순 (금호생명과학연구소, 전남대학교) ;
  • 박현용 (조선대학교 자연과학대학 생물학과)
  • Published : 2007.02.28
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Abstract

Plant Regeneration via organogenesis from leaf disk of Korean dandelion was investigated. Leaf disk cultured on MS medium with various combinations of BA (0-4 mg/L) and 2,4-D (0-1 mg/L). Shoot regeneration from leaf explant was observed after 3 weeks of culture. The highest shoot regeneration frequency from leaf disk was obtained with 2 mg/L BA. To analyze the effect of leaf age along shoot formation, we measured number of shoots per explant, shooting rate, fresh and dry weight of leaf explant. The highest number of shoots (11.5) per explant were obtained leaf from 7 weeks old plantlets after seed germination. The regenerated shoots were transferred in 1/2 MS medium with 0.5 mg/L NAA for root formation. Regeneratied plantlets thought organogenesis were growing to whole plants in the pots with acclimation.

한국의 전통적인 민간 약재로 사용되어져온 흰민들레의 잎절편체로 부터 기관형성을 통한 재분화를 시도하였다. 잎 절편체를 BA (0-4mg/L)와 2,4-D (0-1mg/L)가 혼합 첨가된 MS 기본 배지에 치상하여 배양한 결과, 배양 3주후부터 신초가 발생되었다. 신초 분화율은 2mg/L BA 첨가된 조건에서 가장 높았으며, 배양 4주째 4mg/L BA 첨가된 조건에서는 캘러스가 유기되었다. 잎령에 따른 부정아 형성율, 발생빈도, 건량, 생중량을 조사한 결과, 발아 후 7주된 잎 절편체에서 평균 11.5개의 신초를 형성하였다. 유도된 신초는 0.5mg/L NAA가 첨가된 배지에서 발근되었으며, 화분에 옮겨 완전한 식물체로 재분화 되었다.

Keywords

References

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