Component Analysis and Antioxidant Activity of Adenophora triphylla

잔대의 함유성분 분석과 항산화 활성

The levels of minerals, amino acids, fatty acids and sugars and antioxidant capacity of Adenophora triphylla (AT) root were investigated. The moisture, crude protein, crude lipid, crude ash and carbohydrate contents of the AT root were $3.4{\pm}0.3,\;9.4{\pm}0.1,\;1.6{\pm}0.1,\;3.7{\pm}0.2$, and $81.9{\pm}0.5$, respectively. Among the minerals present in root, potassium content was the highest. Total amino acid contents were $103.5{\pm}9.1$ mg/g. Palmitic acid was the most abundant fatty acid in AT root. The vitamin E and C levels in AT root were $1.1{\pm}0.1$ and $5.2{\pm}0.5$ mg/100 g, respectively. The antioxidant activity of 70% ethanol extract and fractions of the AT root has been determined by the scavenging of the stable radical DPPH; the result showed that the chloroform fraction was the most active, as the amount required for 50% reduction of DPPH after 30 min ($RC_{50}$) was $103.7{\mu}g$, followed by 70% ethanol extract ($265.4{\mu}g$), hexane fraction ($104.4{\mu}g$), ethyl acetate fraction ($104.4{\mu}g$), butanol fraction ($108.9{\mu}g$) and aqueous fraction ($484.1{\mu}g$).

잔대 뿌리의 무기질, 아미노산, 지방산, 당류 함량과 항산화 효과에 대해서 조사하였다. 수분, 조단백 조지방, 조회분과 탄수화물은 각각 $3.4{\pm}0.3,\;9.4{\pm}0.1,\;1.6{\pm}0.1,\;3.7{\pm}0.2$와 $81.9{\pm}0.5%$이었다. 무기질 중에 칼륨의 함량이 가장 높게 나타났으며, 총 아미노산 함량은 $103.5{\pm}9.1$ mg/g이었고, 지방산에서 palmitic acid가 가장 많았다. 잔대 뿌리에 함유된 비타민 E와 C는 각각 $1.1{\pm}0.1$과 $5.2{\pm}0.5$ mg/100 g이었다. 잔대 70% 에탄올, 헥산, 클로로포름, 에틸아세테이트, 부탄올과 물 추출물에서 DPPH 라디칼 소거능의 항산화 효과 결과는 $RC_{50}$값이 265.4, 117.1, 103.7, 104.4, 108.9, 484.1 ${\mu}g$로 나타났다.