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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Transcriptional activation of pref-1 by E2F1 in 3T3 L1 cells

  • Shen, Yan-Nan (Department of Biology, Kyung Hee University) ;
  • Kim, Yoon-Mo (Department of Biology, Kyung Hee University) ;
  • Yun, Cheol-Heui (Department of Agricultural Biotechnology, Seoul National University) ;
  • Moon, Yang-Soo (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Kim, Sang-Hoon (Department of Biology, Kyung Hee University)
  • Published : 2009.10.31
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Abstract

The E2F gene family appears to regulate the proliferation and differentiation of events that are required for adipogenesis. Pref-1 is a transmembrane protein that inhibits adipocyte differentiation in 3T3-L1 cells. In this study, we found that the expression of pref-1 is regulated by the transcription factor E2F1. The expression of pref-1 and E2F1 was strongly induced in preadipocytes and at the late differentiation stage. Using luciferase reporter assay, ChIP assay and EMSA, we found that the -211/-194 region of the pref-1 promoter is essential for the binding of E2F1 as well as E2F1-dependent transcriptional activation. Knockdown of E2F1 reduced both pref-1 promoter activity and the level of pref-1 mRNA. Taken together, our data suggest that transcriptional activation of pref-1 is stimulated by E2F1 protein in adipocytes.

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References

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