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Statistical optimization of culture media contained soy proteins and hypocotyl for the growth of Bifidobacterium lactis BL 740 and production of soy isoflavone aglycones

대두 단백질 및 배아를 이용한 Bifidobacterium lactis BL740의 균체성장 및 이소플라본 비배당체 생산를 위한 통계적 배지 최적화

  • Lee, Choong-Young (Central Research Institute, Dr. Chung's Food Co. Ltd.) ;
  • Lee, Yoon-Bok (Central Research Institute, Dr. Chung's Food Co. Ltd.) ;
  • Lee, Keun-Ha (Central Research Institute, Dr. Chung's Food Co. Ltd.) ;
  • Park, Myeong-Soo (Department of Hotel Culinary Arts, Anyang Technical College) ;
  • Hwang, Seock-Yeon (Department of Biomedical Laboratory Science College of Applied Science and Industry, Daejeon University) ;
  • Hong, Seung-Bok (Department of Clinical Laboratory Science, Juseong University) ;
  • Yoo, Yung-Choon (Department of Microbiology, College of Medicine, Konyang University) ;
  • Yu, Byung-Yeon (Department of Family Medicine, College of Medicine, Konyang University) ;
  • Kim, Chung-Ho (Department of Food and Nutrition, Seowon University)
  • 이충영 ((주)정.식품 종양연구소) ;
  • 이윤복 ((주)정.식품 종양연구소) ;
  • 이근하 ((주)정.식품 종양연구소) ;
  • 박명수 (안양과학대학 호텔 관광학과) ;
  • 황석연 (대전대학교 임상병리학과) ;
  • 홍승복 (주성대학교 임상병리학과) ;
  • 유영춘 (건양대학교 의과대학 미생물학과) ;
  • 유병연 (건양대학교 의과대학 가정의학과) ;
  • 김정호 (서원대학교 식품영양학과)
  • Received : 2010.06.03
  • Accepted : 2010.06.21
  • Published : 2010.09.30

Abstract

In order to maximize the growth of Bifidobacterium lactis BL 740 and soy isoflavone agycones production, we investigated the optimization of a culture medium containing soy hypocotyls, which are the byproducts of the soy manufacturing process, and soy proteins. The ingredients of the medium containing soy materials (S-medium) were selected by fractional factorial design (FFD) and central composite design (CCD) within a desirable range. The FFD was applied by six factors: glucose, cellobiose, fructooligosaccharide, soy peptone, soy protein, and soy hypocotyl. Soy protein, soy peptone, and soy hypocotyl were found to be significant factors from the result of FFD for both the growth of B. lactis BL 740 and aglycone production. The CCD was then applied with three variables found from FFD at five levels each and the optimum values were determined for the three variables: soy peptone, soy protein, and soy hypocotyl. In the case of the growth of B. lactics BL740, the proposed optimal media contained 12.73 g/L of soy protein, 29.55 g/L of soy peptone, and 130.67 g/L of soy hypocotyl. To produce isoflavone aglycones, optimized media was composed of 2.06 g/L, soy protein, 1.25 g/L of soy peptone, and 60.02 g/L of soy hypocotyl.

대두가공공정 중 발생하는 부산물인 대두배아 및 대두 단백질을 이용하여 Bifidobacterium lactis BL740의 생균수 및 대두 이소플라본 비배당체 생산 최대화를 위한 배지최적화를 수행하였고 이를 위하여 통계적 방법인 fractional factorial design (FFD) 및 central composite design(CCD)를 이용, 대두 유래 성분들이 포함된 배지 (S-medium)의 최적 조성물을 확언하였다. FFD의 경우 glucose, cellobiose, fructooligosaccharidde, soy peptone, soy protein, 대두배아를 이용 총 6가지 요소를 2수준에서 적용하였으며 이 중 soy protein, soy peptone 그리고 대두 배아가 B. lactis BL740의 균성장 혹은 대두 이소플라본 비배당체 전환에 중요 인자로 확인이 되었다. FFD에서 확인된 3가지 인자를 CCD에 적용하였으며 이를 통해 균성장의 경우 최적의 배지조성함량이 soy peptone 29.55 g/L, soy protein 12.73 g/L, 대두 배아 130.67 g/L로 확인되었으며 비배당체 전환의 경우 soy peptone 1.25 g/L, soy protein 2.06 g/L, 대두 배아 60.02 g/L로 최적화 되었다.

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