Korean Journal of Materials Research (한국재료학회지)

Materials Research Society of Korea (한국재료학회)
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Biocompatibility of Multilayer Poly Methyl Methacrylate (PMMA)/Poly Vinyl Alcohol (PVA) Bone Plate by Electrospinning Method

전기방사로 제조된 다층 Poly Methyl Methacrylate (PMMA)/Poly Vinyl Alcohol (PVA) Bone Plate의 생체적합성 평가

  • Kwak, Kyung-A (Department of Microbiology, School of Medicine, Soonchunhyang University) ;
  • Kim, Young-Hee (Department of Microbiology, School of Medicine, Soonchunhyang University) ;
  • Thai, Van Viet (Department of Biomedical Engineering, School of Medicine, Soonchunhyang University) ;
  • Lee, Byong-Taek (Department of Biomedical Engineering, School of Medicine, Soonchunhyang University) ;
  • Song, Ho-Yeon (Department of Microbiology, School of Medicine, Soonchunhyang University)
  • 곽경아 (순천향대학교 의과대학 미생물학교실) ;
  • 김영희 (순천향대학교 의과대학 미생물학교실) ;
  • ;
  • 이병택 (순천향대학교 의과대학 의공학교실) ;
  • 송호연 (순천향대학교 의과대학 미생물학교실)
  • Received : 2010.04.18
  • Accepted : 2010.06.07
  • Published : 2010.06.27
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Abstract

Multilayer Poly methyl methacrylate (PMMA)/ Poly vinyl alcohol (PVA) bone plates were fabricated using electrospinning and in vitro investigations were carried out for pre-clinical biocompatibility studies. The initial cellular cytotoxicity of the methacrylate (PMMA)/ Poly vinyl alcohol (PVA) bone plates was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay using fibroblast-like L-929 cells. Cellular adhesion and differentiation studies were carried out using osteoblast-like MG-63 cells. As simulated body fluid (SBF) contains the same ionic concentration of body fluid and any bioactive material tends to deposit bone-like apatite on the samples surfaces into the SBF, in vitro bioactivity of the multilayer bone plates were investigated using SBF. We also studied the internal organization and tensile strength of the multilayer PMMA/PVA bone plates using micro-computed topography (${\mu}$-CT) and universal testing instrument (UTI, Korea) respectively. The cellular cytotoxicity study with MTT confirmed that the cellular viability was 78 to 90% which indicates good cyto-compatibility. Scanning electron microscopic findings revealed a good attachment and adhesion phenomenon of MG-63 cells onto the surfaces of the samples. Cellular differentiation studies also showed that osteogenic differentiation was switched on in a timely manner and affirmed along with that of the control group. Bone-like apatite formation on the surfaces was confirmed within 14 days of SBF incubation. Initial organizations of the multilayer PMMA/PVA bone plates were characterized as dense and uniform. The tensile strength of the post-pressing electronspun mat was higher than that of the pre-electronspun mat. These results suggest that a multilayer PMMA/PVA bone plate system is biocompatible, bioactive and a very good alternative bone plate system.

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References

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