Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Evaluation of Solvent Extraction on the Anti-Inflammatory Efficacy of Glycyrrhiza uralensis

감초의 추출용매별 항염증 효능 평가 연구

  • Yoon, Tae-Sook (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Cheon, Myeong-Sook (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Kim, Seung-Ju (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Lee, A-Yeong (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Moon, Byeong-Cheol (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Chun, Jin-Mi (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Choo, Byung-Kil (Department of Crop Agriculture & Life Science, College of Agriculture & Life Science, Chonbuk National University) ;
  • Kim, Ho-Kyoung (Center of Herbal Resources Research, Korea Institute of Oriental Medicine)
  • 윤태숙 (한국한의학연구원 한약자원연구센터) ;
  • 전명숙 (한국한의학연구원 한약자원연구센터) ;
  • 김승주 (한국한의학연구원 한약자원연구센터) ;
  • 이아영 (한국한의학연구원 한약자원연구센터) ;
  • 문병철 (한국한의학연구원 한약자원연구센터) ;
  • 천진미 (한국한의학연구원 한약자원연구센터) ;
  • 추병길 (전북대학교 농업생명과학대학 작물생명과학과) ;
  • 김호경 (한국한의학연구원 한약자원연구센터)
  • Received : 2009.09.04
  • Accepted : 2010.01.04
  • Published : 2010.02.28
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Abstract

Glycyrrhiza uralensis (Leguminosae) is a well-known herbal medicine that has long been valued as a demulcent to relieve inflammatory disorders. To compare the influence of different solvents on the anti-inflammatory efficacy of G. uralensis, we measured the inhibition of pro-inflammatory mediators such as NO, TNF-$\alpha$, and $PGE_2$ in lipopolysaccharide (LPS)-stimulated mouse macrophage RAW 264.7 cells by extracts produced using different solvents (water, methanol, ethanol, or n-hexane). The results showed that methanol was the most effective solvent for the inhibition of both NO and $PGE_2$ production in RAW 264.7 cells. However, there was no difference among the extracts for inhibition of TNF-$\alpha$. Further study must be performed for the analysis of correlation between the anti-inflammatory activity of extracts produced using different solvents and the content of major bioactive compounds in G. uralensis, such as glycyrrhizin and liquiritin. The present study suggests that methanol may be a more appropriate solvent of G. uralensis than other solvents (water, ethanol, and n-hexane) to yield the greatest anti-inflammatory activity for food additives and medicine.

Keywords

References

  1. Carmichael J, DeGraff WG and Gazdar AF. (1987). Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Research. 47:936-941.
  2. Cheng A, Wan F, Jin Z, Wang J and Xu X. (2008a). Nitrite oxide and inducible nitric oxide synthase were regulated by polysaccharides isolated from Glycyrrhiza uralensis Fisch. Journal of Ethnopharmacology. 118:59-64. https://doi.org/10.1016/j.jep.2008.03.002
  3. Cheng A, Wan F, Wang J, Jin Z and Xu X. (2008b). Macrophage immunomodulatory activity of polysaccharides isolated from Glycyrrhiza uralensis Fisch. International Immunopharmacology. 8:43-50. https://doi.org/10.1016/j.intimp.2007.10.006
  4. Fujiwara N and Kobayashi K. (2005). Macrophages in inflammation. Current Drug Targets - Inflammation and Allergy. 4:281-286. https://doi.org/10.2174/1568010054022024
  5. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS and Tannenbaum SR. (1982). Analysis of nitrate, nitrite, and $[^{15}N]$nitrate in biological fluids. Analytical Biochemistry. 126:131-138. https://doi.org/10.1016/0003-2697(82)90118-X
  6. Kim HP, Son KH, Chang HW and Kang SS. (2004). Antiinflammatory plant flavonoids and cellular action mechanisms. Journal of Pharmacological Sciences. 96:229-245. https://doi.org/10.1254/jphs.CRJ04003X
  7. Lee JH, Ko WS, Kim YH, Kang HS, Kim HD and Choi BT. (2001). Anti-inflammatory effect of the aqueous extract from Lonicera japonica flower is related to inhibition of NF-kappaB activation through reducing I-kappaBalpha degradation in rat liver. International Journal of Molecular Medicine. 7:79-83.
  8. Lee YG and Cho JY. (2007). Inhibitory effects of curcumin on nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 cells and its suppressive mechanism. Korean Journal of Medicinal Crop Science. 15:451-456.
  9. Lin JY, Li CY and Hwang IF. (2008). Characterisation of the pigment components in red cabbage (Brassica oleracea L. var.) juice and their anti-inflammatory effects on LPS-stimulated murine splenocytes. Food Chemistry. 109:771-781. https://doi.org/10.1016/j.foodchem.2008.01.039
  10. Paul A, Cuenda A, Bryant CE, Murray J, Chilvers ER, Cohen P, Gould GW and Plevin R. (1999). Involvement of mitogen activated protein kinase homologues in the regulation of lipopolysaccharide-mediated induction of cyclo-oxygenase-2 but not nitric oxide synthase in RAW 264.7 macrophages. Cellular Signalling. 11:491-497. https://doi.org/10.1016/S0898-6568(99)00018-2
  11. Pulendran B, Palucka K and Banchereau J. (2001). Sensing pathogens and tuning immune responses. Science. 293:253-256. https://doi.org/10.1126/science.1062060
  12. Sabbioni C, Ferranti A, Bugamelli F, Forti GC and Raggi MA. (2006). Simultaneous HPLC analysis, with isocratic elution, of glycyrrhizin and glycyrrhetic acid in liquorice roots and confectionery products. Phytochemical Analysis. 17:25-31. https://doi.org/10.1002/pca.877
  13. Seo JS, Lee TH, Lee SM, Lee SE, Seong NS and Kim J. (2009). Inhibitory effects of methanolic extracts of medicinal plants on nitric oxide production in activated macrophage RAW 264.7 cells. Korean Journal of Medicinal Crop Science. 17:173-178.
  14. Steinman L. (2004). Immune therapy for autoimmune diseases. Science. 305:212-216. https://doi.org/10.1126/science.1099896
  15. Sun C, Xie Y, Tian Q and Liu H. (2008). Analysis of glycyrrhizic acid and liquiritin in liquorice root with microwave-assisted micellar extraction and pre-concentration. Phytochemical Analysis. 19:160-163. https://doi.org/10.1002/pca.1031
  16. Sun HX and Pan HJ. (2006). Immunological adjuvant effect of Glycyrrhiza uralensis saponins on the immune responses to ovalbumin in mice. Vaccine. 24:1914-1920. https://doi.org/10.1016/j.vaccine.2005.10.040
  17. Williamson EM. (2001). Synergy and other interactions in phytomedicines. Phytomedicine. 8:401-409. https://doi.org/10.1078/0944-7113-00060