Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Acupuncture Stimulation to HT8 Enhances Cell Proliferation in Hippocampus on an Epilepsy Mouse Model

마우스 간질 동물모델에서 소부혈 자침이 해마 치상회의 신경세포증식에 미치는 영향

  • Kim, Seung-Tae (Division of meridian and structural medicine, School of Korean medicine, Pusan National University) ;
  • Park, Hae-Jeong (Kohwang Medical Research Institute, Kyung Hee University) ;
  • Hong, Mee-Sook (Kohwang Medical Research Institute, Kyung Hee University) ;
  • Kim, Seung-Nam (Acupuncture & Meridian Science Research Center, Kyung Hee University) ;
  • Doo, Ah-Reum (Acupuncture & Meridian Science Research Center, Kyung Hee University) ;
  • Yin, Chang-Shik (Acupuncture & Meridian Science Research Center, Kyung Hee University) ;
  • Lee, Hye-Jung (Acupuncture & Meridian Science Research Center, Kyung Hee University) ;
  • Chung, Joo-Ho (Kohwang Medical Research Institute, Kyung Hee University) ;
  • Park, Hi-Joon (Acupuncture & Meridian Science Research Center, Kyung Hee University)
  • 김승태 (부산대학교 한의학전문대학원 경락구조의학부) ;
  • 박해정 (경희대학교 고황의학연구소) ;
  • 홍미숙 (경희대학교 고황의학연구소) ;
  • 김승남 (경희대학교 침구경락과학연구센터) ;
  • 두아름 (경희대학교 침구경락과학연구센터) ;
  • 인창식 (경희대학교 침구경락과학연구센터) ;
  • 이혜정 (경희대학교 침구경락과학연구센터) ;
  • 정주호 (경희대학교 고황의학연구소) ;
  • 박히준 (경희대학교 침구경락과학연구센터)
  • Received : 2010.04.30
  • Accepted : 2010.06.09
  • Published : 2010.06.27
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Abstract

목적 : 뇌의 신경세포 증식은 해마 치상회와 뇌실하영역에서만 나타나는 현상이다. Kainic acid(KA)를 이용한 간질 동물모델을 연구하던 중 침이 해마 치상회의 신경세포증식을 촉진하는 현상을 발견하여 이를 보고하고자 한다. 방법 : 수컷 ICR계 생쥐를 Saline(n=8), KA(n=8), KA+Acu(n=8)의 세 군으로 나누고, 모든 생쥐들에게 KA 주입 3일 전부터 1일 1회씩 5'-bromodeoxyuridine(BrdU)을 3일간 주입하였다. Saline군에는 멸균된 생리식염수를 뇌실 내에 주입하였고, KA군 및 KA+Acu군에는 $0.1{\mu}g$의 KA를 뇌실 내에 주입하였으며, KA+Acu군에 속한 쥐들에게는 KA 주입 2일전, 1일전, 주입 직후에 양쪽 소부(少府)(HT8)에 자침하였다. KA 주입 3시간 후 쥐의 뇌를 적출하고 해마 치상회부위의 BrdU 및 neuropeptide Y (NPY)의 발현을 측정하였다. 결과 : 소부(少府) 자침이 KA의 독성으로 인한 신경세포의 파괴를 줄여주었으며, BrdU 양성 세포 및 NPY를 유의하게 증가시켰다. KA 주입시 세포증식이 일어나긴 하나, 3시간 안에는 거의 일어나지 않는다. 결론 : 소부(少府) 자침이 해마 치상회의 신경세포증식을 촉진하며, 이는 KA의 효과가 아닌 KA 투여 전 소부(少府) 자침으로 인한 것으로 사료된다.

Keywords

References

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