Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

Evaluation of Antimicrobial, Antioxidant, and Antithrombin Activities of the Rhizome of Various Dioscorea Species

마 품종별 메탄올 추출물의 항균, 항산화 및 항혈전 활성 평가

  • Kwon, Jung-Bae (Experiment Station of Bioresources, Gyeongbuk Agricultural Research & Extension Services) ;
  • Kim, Mi-Sun (Department of Food and Nutrition, Andong National University) ;
  • Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University)
  • 권중배 (경상북도농업기술원 생물자원연구소) ;
  • 김미선 (안동대학교 식품영양학과) ;
  • 손호용 (안동대학교 식품영양학과)
  • Received : 2010.01.14
  • Accepted : 2010.06.04
  • Published : 2010.06.30
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Abstract

Yams (Dioscorea spp.) have been used as medicinal and edible resources worldwide. In the present study, methanol extracts were prepared from various kinds of yam, including D. batatas (Gyeongbuk No. 1 and No. 4), D. alata L. (Gyeongbuk No. 5 and No. 6), and D. bulbifera and D. nipponica, and the antioxidant, antithrombin, and antimicrobial activities of these extracts were evaluated. The water content of the various yams was 54.7~83.2% (D. batatas> D. alata L.>D. nipponica>D. bulbifera) and the methanol extracts contained 0.59-1.59% (w/v) solid matter (D. alata L.>D. batatas >D. nipponica>D. bulbifera). The mean concentrations of total polyphenols, total flavonoids, and total sugars in the different types of yam were 49.82 mg/g, 9.79 mg/g, and 173.81 mg/g, respectively. Analysis of DPPH radical-scavenging activity showed that D. alata L. Gyeongbuk No. 6 had a strong antioxidant capacity (IC50=$142.30{\mu}g/mL$), whereas the other yams showed reduced levels of activity (IC50=371-$486{\mu}g/mL$). D. alata L. Gyeongbuk No. 6 showed a strong antithrombin activity against human thrombin. Upon treatment with a D. alata methanol extract (1.5 mg/mL), thrombin time was increased 7.35-fold compared with that when methanol alone was used. In an antimicrobial activity assay, the D. nipponica extract showed moderate antibacterial activity against Bacillus subtilis, Staphylococcus aureus, Proteus vulgaris, and Salmonella typhimurium. Our results indicate that different varieties of Dioscorea spp., including D. batatas, have useful biochemical attributes, including antioxidant, antithrombin, and antibacterial activities.

마는 전 세계적으로 오랜 기간 동안 식용 및 약용으로 사용되어 온 중요한 생물자원이다. 본 연구에서는 2009년 경북 안동지역에서 재배된 D. batatas (경북1호 및 4호), D. alata L.(경북5호 및 6호), D. bulbifera (둥근마) 및 D. nipponica (부채마)로부터 메탄올 추출물을 조제하고, 이들의 항산화, 항혈전, 항균 활성을 평가하였다. 먼저 수분함량의 경우 마의 종류에 따라 54.7%∼83.2%로 다양하게 나타났으며 D. batatas>D. alata L.>D. nipponica>D. bulbifera 순으로 나타났으며, 메탄올 추출효율은 0.59%∼1.59%로 D. alata L.>D. batatas>D. nipponica>D. bulbifera 순으로 나타났다. 다양한 마들의 평균 총 폴리페놀, 총 플라보노이드, 총당의 경우 각각 49.82, 9.79 및 173.81 mg/g이었으며, 총 폴리페놀은 둥근 대마(경북6호)에서(87.05 mg/g), 총 플라보노이드는 둥근 대마(12.67 mg/g) 및 둥근마(13.99 mg/g)에서, 총당은 장마(경북4호, 281.96 mg/g)에서 가장 높게 나타났다. DPPH radical 소거능 평가결과, 대부분의 마 시료가 371∼486 $\mu$g/mL의 IC50를 나타내었으나, 둥근 대마의 경우 142.30 $\mu$g/mL의 IC50를 나타내어 우수한 항산화력을 확인하였다. 또한 둥근 대마는 1.5 mg/mL 농도에서 7.35배 증가된 thrombin time을 보여 강력한 트롬빈 저해활성을 나타내었다. 한편 항균력의 경우 부채마에서 우수하였으며, B. subtilis, S. aureus, P. vulgaris 및 S. typhimurium에 대한 양호한 항세균 활성을 나타내었다. 본 연구결과는 국내에서 주로 재배되고 있는 D. batatas (경북1호 및 4호) 이외의 다양한 마의 유용성을 제시하고 있다.

Keywords

References

  1. Yang, M.H., Yoon, K.D., Chin, Y.W. and Kim, J.W. (2009) Phytochemical and pharmacological profiles of Dioscorea species in Korea, China and Japan. Korean J. Pharmacogn., 40, 257-279
  2. Ahn, J.H., Son, K.H., Sohn, H.Y. and Kwon, S.T. (2005) In vitro culture of adventitious roots from Dioscorea nipponica Makino for the production of steroidal saponins. Korean J. Plant Biotechnol., 32, 317-323
  3. Kum, E.J., Park, S.J. Lee, B.H. Kim, J.S., Son, K.H. and Sohn, H.Y. (2006) Antifungal activity of phenanthrene derivatives from aerial bulbils of Dioscroea batatas Decne. J. Life Sci., 16, 647-652 https://doi.org/10.5352/JLS.2006.16.4.647
  4. Kim, J.I., Jang, H.S., Kim, J.S. and Sohn, H.Y. (2009) Evaluation of antimicrobial, antithrombin, and antioxidant activity of Dioscorea batatas Decne. Korean J. Microbiol. Biotechnol., 37, 133-139
  5. Kwon, C.S., Sohn, H.Y., Kim, S.H., Kim, J.H., Son, K.H., Lee, J.S., Lim, J.K. and Kim, J.S. (2003) Anti-obesity effect of Dioscorea nipponica Makino with lipase-inhibitory activity in rodents. Biosci. Biotechnol. Biochem., 67, 1451-1456 https://doi.org/10.1271/bbb.67.1451
  6. Son, I.S., Kim, J.I., Sohn, H.Y., Son, K.H., Kim, J.S. and Kwon, C.S. (2007) Antioxidative and hypolipidemic effects of diosgenin, a steroidal saponin of yam (Dioscorea spp), on hing-cholesterol fed rats. Biosci. Biotechnol. Biochem., 71, 3063-3071 https://doi.org/10.1271/bbb.70472
  7. Kang, T.H., Choi, S.Z., Lee, T.H., Son, M.W. and Kim. S.Y. (2008) Characteristics of antidiabetic effect of Dioscorea rhizoma (1) - Hypoglycemic effect. Korean J. Food Nutr., 21, 425-429
  8. Kang, T.H., Choi, S.Z., Lee, T.H., Son, M.W. and Kim. S.Y. (2008) Characteristics of antidiabetic effect of Dioscorea rhizoma (2)- prevention of diabetic neuropathy by NGF induction. Korean J. Food Nutr., 21, 430-435
  9. Kim M. W. (2001) Effects of $H_2O$-fraction of Dioscorea japonica Thunb and selenium on lipid peroxidation in streptozotocin-induced diabetic rats. Korean J. Soc. Food Cookery Sci., 17, 344-352
  10. Kwon, C.S., Son, I.S., Shim, H.Y., Kwu, I.S. and Chung, K.M. (1999) Effects of yam on lowering cholesterol level and its mechanism. Korean J. Food Nutr., 32, 637-643
  11. Kwon, E.G., Choe, E.M. and Gu. S.J. (2001) Effects of mucilage from yam (Dioscorea batatas DECNE) on blood glucose and lipid composition in alloxan-induced diabetic mice. Korean J. Food Sci. Technol., 33, 795-801
  12. Lee, I.S., Chung, S.Y., Shim, C.S. and Koo, S.J. (1995) Inhibitory effects of yam (Dioscorea batatas DECNE) extracts on the mutagenicity. Korean J. Soc. Food Sci., 11, 351-355
  13. Han, Y.N., Hahn, S.H. and Lee, I.R.. (1990) Purification of mucilages from Dioscorea batatas and D. japonica and their content analysis. Korean J. Pharmacogn., 21, 274-283
  14. Kim, J.O., Jung, M.J., Choi, H.J., Lee, J.T., Lim, A.K., Hong, J.H. and Kim, D.I. (2008) Antioxidant and biological activity of hot water and ethanol extracts from Phellinus linteus. J. Korean Soc. Food Sci Nutr., 37, 684-690 https://doi.org/10.3746/jkfn.2008.37.6.684
  15. Sohn, H.Y., Kwon, C.S., Son, K.H., Kwon, G.S., Ryu, H.Y. and Kum, E.J. (2006) Antithrombin and thrombosis prevention activity of buckwheat seed, Fagopyrum esculentum Moench. J. Korean Soc. Food Sci. Nutr., 35, 132-138 https://doi.org/10.3746/jkfn.2006.35.2.132
  16. Sohn, H.Y. Ryu, H.Y., Jang, Y.J, Jang, H.S., Park, Y.M. and Kim, S.Y. (2008) Evaluation of antimicrobial, antithrombin, and antioxidant activity of aerial part of Saxifraga stolonifera. Korean J. Microbiol. Biotechnol., 36, 195-200
  17. Sohn, H.Y., Son, K.H., Kwon, C.S., Kwon, G.S. and Kang, S.S. (2004) Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera (L.) Vent, Sophora flavescens Ait and Echinosophora koreensis Nakai. Phytomedicine, 11, 666-672 https://doi.org/10.1016/j.phymed.2003.09.005
  18. Woo, S.S., Park, Y.K., Choi, C.H., Hahm, K.S. and Lee, D.G. (2007) Mode of antibacterial action of a signal peptide, $Pep^27$from Streptococcus pneumoniae. Biochem. Biophys. Res. Commun., 363, 806-810 https://doi.org/10.1016/j.bbrc.2007.09.041
  19. Liu, Y.H., Lin, Y.S., Liu, D.Z., Han, C.H., Chen, C.T., Fan, M. and Hou, W.C..(2009) Effects of different types of yam (Dioscorea alata) products on the blood pressure of spontaneously hypertensive rats. Biosci. Biotechnol. Biochem., 73, 1371-376 https://doi.org/10.1271/bbb.90022
  20. Liu, Y.W., Shang, H.F., Wang, C.K., Hsu, F.L. and Hou, W.C. (2009) Immunomodulatory activity of dioscorin, the storage protein of yam (Dioscorea alata cv. Tainong No. 1) tuber. Food Chem. Toxicol., 45, 2312-2318
  21. Cheng, W.Y., Kuo, Y.H., Huang, C.J. (2007) Isolation and identification of novel estrogenic compounds in yam tuber (Dioscorea alata Cv. Tainung No. 2). J. Agric. Food Chem., 55, 7350-7358 https://doi.org/10.1021/jf0711690
  22. Kumar, P., Sharma, B. and Bakshi, N. (2009) Biological activity of alkaloids from Solanum dulcamara L. Nat. Prod. Res., 23, 719-723 https://doi.org/10.1080/14786410802267692