Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

Effects of Scutellariae Radix on Locomotor Dysfunction of Contusive Spinal Cord Injury Induced Rats

황금(黃芩)이 척수압박손상 흰쥐의 운동기능장애에 미치는 영향

  • Kim, Eun-Seok (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kim, Pyung-Soo (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kim, Bum-Hoi (Department of Anatomy, College of Oriental Medicine, Dong-eui University) ;
  • Shin, Jung-Won (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kim, Seong-Joon (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Sohn, Nak-Won (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
  • 김은석 (경희대학교 동서의학대학원 한의과학) ;
  • 김평수 (경희대학교 동서의학대학원 한의과학) ;
  • 김범회 (동의대학교 한의과대학 해부학교실) ;
  • 신정원 (경희대학교 동서의학대학원 한의과학) ;
  • 김성준 (경희대학교 동서의학대학원 한의과학) ;
  • 손낙원 (경희대학교 동서의학대학원 한의과학)
  • Received : 2011.05.17
  • Accepted : 2011.05.26
  • Published : 2011.06.20
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Abstract

Objectives : This study was performed to evaluate the effects of Scutellariae radix (SR) water extract on locomotor dysfunction induced by spinal cord injury (SCI) in rats. Methods : SCI was induced mechanical contusion following laminectomy of 10 th thoracic vertebra in Sprague-Dawley rats. SR was orally given once a day for 7 days after SCI. Neurological behavior was examined with the Basso-Beattie-Bresnahan locomotor rating scale. Tissue damage and nerve fiber degeneration were examined with cresyl violet and luxol fast blue (LFB) histochemistry. Using immunohistochemistry, cellular damages to neurons and nerve fibers were examined MAP-2. Results : 1. SR significantly ameliorated the locomotor dysfunction of the SCI-induced rats. 2. SR significantly reduced the number of motor neurons in the ventral horn of the SCI-induced rat spinal cord. 3. SR attenuated the reduction of nerve fiber shirnakage and degeneration of the SCI-induced rat spinal cord. 4. SR attenuated the reduction of MAP-2 positive cells in the peri-lesion of the SCI-induced rat spinal cord. Conclusions : These results suggest that SR improves the locomotor dysfunction of SCI by reducing degeneration of nerve fibers and motor neuron shrinkage in the ventral horn.

Keywords

References

  1. Anthony M Choo, Jie Liu, Marcel Dvorak, Wolfram Tetzlaff, Thomas R Oxland. Secondary pathology following contusion, dislocation, and distraction spinal cord injuries. Exp Neurol. 2008 ; 212 : 490-506. https://doi.org/10.1016/j.expneurol.2008.04.038
  2. Manhong Ma, D Michele Basso, Patricia Walters, Bradford T Stokes, and Lyn B Jakeman. Behavioral and Histological Outcomes Following Graded Spinal Cord Contusion Injury in the C57Bl/6 Mouse. Exp. Neurol. 2001 ; 169 : 239-54. https://doi.org/10.1006/exnr.2001.7679
  3. 대한척추신경외과학회. 척추학. 서울 : 군자출판사. 2008 : 561-70.
  4. Brian K Kwon, Wolfram Tetzlaff, Jonathan N Grauer, John Beiner, Alexander R Vaccaro. Pathophysiology and pharmacologic treatment of acute spinal cord injury. Spine J. 2004 ; 4 : 451-64. https://doi.org/10.1016/j.spinee.2003.07.007
  5. 한방재활의학과학회. 한방재활의학. 서울 : 군자출판사. 2005 : 133-5.
  6. 김동휘, 손홍문, 김종중, 하상호, 이상홍, 문영래, 이준영, 임만택, 유재원. 척수손상 쥐에 사람탯줄 혈액 이식 후 손상 부위의 조직학적 변화와 행동 변화. 대한척추외과학회지. 2007 ; 14 : 8-16.
  7. Young W. Experimental spinal cord injury therapies 4th International Neurotrauma symposium (proceedings) PS8-2.
  8. 윤영제, 이정훈, 이병훈. 백서의 척수손상 후 중강도 저체온법과 수중운동이 운동기능에 미치는 영향. 한국콘텐츠학회논문지. 2010 ; 10 : 206-15.
  9. 강봉주, 조동욱, 홍성길. 저산소상태에서 육미지황원의 뇌신경세포 보호효과에 대한 연구. 한국한의학연구원논문집. 2001 ; 7 : 115-24.
  10. 강승준, 금현수, 전연이, 이은주, 박치상, 박창국. 석창포가 뇌허혈을 유발시킨 백서에서의 뇌신경 보호효과. 대한한방내과학회지. 2001 ; 22 : 341-51.
  11. 정춘근, 김은영, 신정원, 손영주, 이현삼, 정혁상, 손낙원. 청폐사간탕이 당뇨유발 흰쥐의 뇌허혈손상에 미치는 영향. 대한한의학회지. 2005 ; 26 : 217-30.
  12. 박원상, 김은석, 신정원, 김범회, 김성준, 강희, 손낙원. 구척이 흰쥐의 척수압박에 의한 신경세포손상에 미치는 영향. 한방재활의학과학회지. 2010 ; 20 : 1-15.
  13. 신민교. 임상본초학. 서울 : 영림사. 1986 : 401.
  14. Seok-Bin Yoon, Young-Jong Lee, Seong Kyu Park, Ho-Cheol Kim, Hyunsu Bae, Hyung Min Kim, Seong-Gyu Ko, Ho Young Choi, Myung Sook Oh, Wansu Park. Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS- activated RAW264.7 Macrophages. J Ethnophar. 2009 ; 125 : 286-90. https://doi.org/10.1016/j.jep.2009.06.027
  15. Joungil Choi, Craig C Conrad, Christina A Malakowsky, John M Talent, Chun-Su Yuan, Robert W Gracy. Flavones from Scutellaria baicalensis Georgi attenuate apoptosis and protein oxidation in neuronal cell lines. Biochimica et Biophysica Acta. 2002 ; 1571 : 201-10. https://doi.org/10.1016/S0304-4165(02)00217-9
  16. 정승현, 신길조, 이원철, 김성배. 배양한 흰쥐 대뇌세포의 저산소증 모델에서 황금(黃芩)이 유전자 표현에 미치는 영향. 대한한방내과학회지. 2004 ; 25 : 324-36.
  17. 임강현, 김영옥, 이세나, 김명규, 부영민, 김선여, 김호철. 뇌허혈로 인한 흰쥐에서의 기억력 및 학습효과 저해에 대한 황금의 보호효과. 대한본초학회지. 2006 ; 21 : 189-95.
  18. 김완식, 정승현, 신길조. 흰쥐 대뇌세포의 저산소 증 모델에서 황금(黃芩)에 의한 신경세포사 저해 효과. 생명과학회지. 2007 ; 81 : 143-9.
  19. Basso DM, Beattie MS, Bresnahan JC. A sensitive and reliable locomotor rating scale for open field testing in rats. J Neurotrauma. 1995 ; 12 : 1-21. https://doi.org/10.1089/neu.1995.12.1
  20. Young W. Spinal cord contusion models. Prog Brain R. 2002 ; 137 : 231-55.
  21. Martinez M, Brezun JM, Bonnier L, Xerri C. A new rating scale for open-field evaluation of behavioral recovery after cervical spinal cord injury in rats. J Neurotrauma. 2009 ; 26(7) : 1043-53. https://doi.org/10.1089/neu.2008.0717
  22. 대한정형외과학회. 정형외과학. 제6판. 서울 : 최신의학사. 2006 : 995-6.
  23. Tator CH. Biology of neurological recovery and functional restoration after spinal cord injury. Neurosurg. 1998 ; 42 : 696-707. https://doi.org/10.1097/00006123-199804000-00007
  24. Sanchez Mejia RO, Ona VO, Li M, Friedlander RM. Minocycline reduces traumatic brain injurymediated caspase-1 actvation, tissue damage, and neurological dysfunction. Neurosurg. 2001 ; 48 : 1393-9.
  25. 김정호. 교통사고로 인한 경추부 손상으로 내원한 환자 1례에 대한 증례보고. 대한침구학회. 대한침구학회지. 2003 ; 20 : 201-9.
  26. 설재욱, 신미숙, 최진봉. 전침요법을 병용한 척추 손상 환자의 치험 1례.한방재활의학과학회. 한방재활의학과학회지. 2004 ; 14 : 117-27.
  27. 전국한의과대학 공동교재편찬위원회. 본초학. 서울 : 영림사. 2004 : 218-20.
  28. 김성배, 정승현, 신길조, 이원철. 배양한 흰쥐 대뇌세포의 저산소증 모델에서 황금이 유전자 표현에 미치는 영향. 대한한방내과학회지. 2004 ; 25 : 324-36.
  29. Crowe MJ, Bresnahan JC, Shuman SL, Masters JN, Beatie MS. Apoptosis and delayed degeneration after spinal cord injury in rats and monkeys. Nat Med. 1997 ; 3 : 73-6. https://doi.org/10.1038/nm0197-73
  30. Emery E, Aldana P, Bunge MB, Puckett W, Srinivasan A, Keane RW, Bethea J, Levi AD. Apoptosis after traumatic human spinal cord injury. J Neurosurg. 1998 ; 89 : 911-20. https://doi.org/10.3171/jns.1998.89.6.0911
  31. Kakulas BA. A review of the neuropathology of human spinal cord injury with emphasis on special features. J Spinal Cord Med. 1999 ; 22 : 119-24.
  32. Dayhoff J, Hameroff S, Lahoz-Beltra R, Swenberg C. Cytoskeletal involvement in neuronal learning: a review. Eur Biophys J. 1994 ; 23 : 79-93.
  33. Diaz-Nido J, Hernandez M, Avila J. Microtubule proteins in neuronal cells. CRC Press Broca Raton FL USA. 1990 : 193-259.
  34. Johnson GV, Jope RS. The role of microtubule- associated protein 2(MAP2) in neuronal growth, plasticity, and degeneration. J Neurosci R. 1992 ; 33 : 505-12. https://doi.org/10.1002/jnr.490330402
  35. Olmsted, JB. Microtubule-associated proteins. Annu Rev Cell Biol. 1986 ; 2 : 421-57. https://doi.org/10.1146/annurev.cb.02.110186.002225
  36. Malinak C, Silverstein FS. Hypoxia-ischemic injury acutely disrupts microtubule-associated protein 2 immunostainning in neonatal rat brain. Biol Neonate. 1996 ; 69 : 257-67. https://doi.org/10.1159/000244319
  37. McRae A, Giland E, Hagberg H. Microglia activation after neonatal hypoxic-ischemia. Dev Brain R. 1995 ; 84 : 245-52. https://doi.org/10.1016/0165-3806(94)00177-2