Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidative Effects of Solvent Fractions from Nandina domestica Fruits

남천(Nandina domestica) 열매 분획 추출물의 항산화 효과

  • Seo, Soo-Jung (Dept. of Herbal Biotechnology, Daegu Haany University) ;
  • Shim, Kyu-Bong (Dept. of Herbal Biotechnology, Daegu Haany University) ;
  • Kim, Nam-Woo (Dept. of Herbal Biotechnology, Daegu Haany University)
  • 서수정 (대구한의대학교 한약자원학과) ;
  • 심규봉 (대구한의대학교 한약자원학과) ;
  • 김남우 (대구한의대학교 한약자원학과)
  • Received : 2011.07.20
  • Accepted : 2011.09.01
  • Published : 2011.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to compare the antioxidative effects of various Nandina domestica fruits extracts. Organic fractions, including n-hexane, chloroform, ethyl acetate, and butanol fractions, were obtained from the water and ethanol extracts of N. domestica fruits. The phenolic compound content of the EE fraction was 922.22 mg/g. The flavonoid compound content was highest in the EB fraction (282.49 mg/g). The electron-donating ability was highest (93%) in the WA and EH fractions at 0.1 mg/mL. The SOD-like activity was showed the highest in the EA fraction (56.36%), and EH and EC both showed higher than 50% activity. The nitrite-scavenging ability of the EC fraction at 1.0 mg/mL under pH 1.2 conditions was 82.03%. The xanthine oxidase inhibitory activities of all the fractions, except WE, were higher than 90% at 0.5 mg/mL. The effect of tyrosinase inhibition was highest in the WE fraction (46.75%). These results show that the N. domestica fruits fractions contained high levels of polyphenol and flavonoid compounds, along with excellent antioxidative effects. This suggests that N. domestica fruits can be used as a functional material.

남천(Nandina domestica) 열매를 물과 에탄올 추출 후 극성이 상이한 유기용매를 순차적으로 분획하여 각 추출물의 생리활성을 측정하였다. 폴리페놀은 EE(ethyl acetate fraction of ethanol extract)에서 922.22 mg/g이었으며, 플라보노이드는 EB(butanol fraction of ethanol extract)에서 282.49 mg/g을 함유하였다. WA(aqueous of water extract)와 EH(n-hexane fraction of ethanol extract)는 0.1 mg/mL에서 93% 이상의 전자공여활성을 나타내었으며, 분획물의 농도 증가에 따른 전자공여능의 유의적 차이는 없었다. SOD 유사활성은 1.0 mg/mL의 EA(aqueous of ethanol extract)에서 56.36%로 가장 우수하였으며, EH와 EC(chloroform fraction of ethanol extract)에서도 50% 이상의 활성을 나타내었다. 아질산염 소거는 pH 1.2의 EC에서 82.03%로 가장 높았다. Xanthine oxidase 저해율은 0.5 mg/mL에서 WE를 제외한 모든 분획물에서 90% 이상의 저해율을 나타내었으나, WE는 46.75%로 가장 우수한 tyrosinase 저해효과를 나타내었다. 이상의 결과 남천 열매 분획물은 다량의 폴리페놀과 플라보노이드를 함유하고 생리활성 효과가 우수하여 천연 항산화 제재 및 이를 이용한 의약품 개발 가능성을 지닌 약용 식물자원인 것으로 판단된다.

Keywords

References

  1. Kinsella JE, Frankel E, German B, Kanner J. 1993. Possible mechanism for the protective role of antioxidants in wine and plant foods. Food Technol 47: 85-89.
  2. Lee YS, Joo EY, Kim NW. 2005. Antioxidant activity of extract from the Lespedeza bicolor. Korean J Food Preserv 12: 75-79.
  3. Kalt W. 2005. Effects of production and processing factors on major fruit and vegetable antioxidant. J Food Sci 70: 11-19. https://doi.org/10.1111/j.1365-2621.2005.tb09053.x
  4. Omaye ST, Reddy KA, Cross CE. 1997. Effect of butylated hydroxytolune and other antioxidants on mouse lung metabolism. J Toxicol Environ Health 3: 829-836.
  5. Yun JB. 2004. The wood easily to fide. Jinseon publishers, Seoul, Korea. p 557.
  6. Shoji N, Umeyama A, Takemoto T, Ohizumi Y. 1984. Serotonergic receptor antagonist from Nandina domestica Thunberg. J Pharm Sci 73: 568-570. https://doi.org/10.1002/jps.2600730435
  7. Park JH. 2002. The encyclopedia of Chinese crude drugs. Shinil publishers, Seoul, Korea. p 122.
  8. Vivek K, Atiqur R, Kang SC. 2008. Chemical composition and inhibitory parameters of essential oil and extracts of Nandina domestica Thunb. to control food-borne pathogenic and spoilage bacteria. Int J Food Microbio 125: 117-122. https://doi.org/10.1016/j.ijfoodmicro.2008.03.011
  9. Ivorra MD, Lugnier C, Schott C, Catret M, Anselmi E, D'Ocon MP. 1992 Investigation of the dual contractile/relaxant properties showed by antiquine in rats aorta. Br J Pharmacol 109: 502-509.
  10. Olechno JD, Poulton JE, Conn EE. 1984. Nandinin: An acylated free cyanohydrine from Nandina domestica. Phytochemistry 23: 1784-1785. https://doi.org/10.1016/S0031-9422(00)83491-3
  11. Woldemeskel M, Styer EL. 2010. Feeding behavior-related toxicity due to Nandina domestica in Cedar Waxwings (Bambycilla cedrorum). Veteri Medi Internat 2010: 1-4.
  12. Indra B, Matsunaga K, Hoshino O, Suzuki M, Ogasawara H, Ohizumi Y. 2002. Structure-activity relationship studies with (${\pm}$)-nantenine derivatives for ${\alpha}_1$-adrenoceptor antagonist activity. Eur J Pharmacol 437: 173-178. https://doi.org/10.1016/S0014-2999(02)01303-1
  13. Legendra O, Pecic S, Chaudhary S, Zimmerman SM, Fantegrossi WE, Harding WW. 2010. Synthetic studies and pharmacological evaluations on the MDMA (Ecstasy) antagonist nantenine. Bioorganic Med Chem Lett 20: 628-631. https://doi.org/10.1016/j.bmcl.2009.11.053
  14. AOAC. 2005. Official method of analysis. 18th ed. Association of Official Analytical Chemists, Washington, DC, USA. Vol 45, p 21-22.
  15. Nieva Moreno MI, Isla MI, Sampietro AR, Vattuone MA. 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71: 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  16. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  17. Marklund S, Marklund G. 1975. Involvement of superoxide amino radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 468-474.
  18. Kato H, Lee IE, Chuyen NV, Kim SB, Hayase F. 1987. Inhibition of nitrosamine formation by nondialyzable melanoidins. Agric Biol Chem 51: 1333-1338. https://doi.org/10.1271/bbb1961.51.1333
  19. Stirpe F, Corte ED. 1969. The regulation of rat liver xanthine oxidase. J Biol Chem 244: 3855-3861.
  20. Yagi A, Kanbara T, Morinobu N. 1987. Inhibition of mushroom-tyrosinase by aloe extract. Planta Med 53: 517-519. https://doi.org/10.1055/s-2006-962799
  21. Park HS. 2011. Comparison of antioxidant activities of wild grape seed (Vitis coignetiea seed) extracts by solvents. Korean J Cul Res 17: 270-279.
  22. Kang SK, Jeong CH, Heo HJ, Shim KH. 2010. Antioxidative activities of various solvent fractions from fruit and leaf of Pinkpop Borisu. J Agric Life Sci 44: 69-78.
  23. Choi SY, Cho HS, Sung NJ. 2006 The antioxidative and nitrite scavenging ability of solvent extracts from wild grape (Vitis coignetiea) skin. J Korean Soc Food Sci Nutr 35: 961-966. https://doi.org/10.3746/jkfn.2006.35.8.961
  24. Kim SA, Oh SI, Lee MS. 2007. Antioxidative and cytotoxic effects of solvents fractions from Elaeagnus multiflora. Korean J Food and Nutr 20: 134-142.
  25. Chung IM, Kim KH, Ahn JK. 1998. Screening of Korean medicinal and food plants with antioxidant activity. Korean J Med Crop Sci 6: 311-322.
  26. Park Ch, Kim DH, Kim ML. 2008. Biological activities of extracts from Corni fructus, Astragalus membranaceus and Glycyrrhiza uralensis. Kor J Herbology 23: 93-101.
  27. Park YS, Chang HG. 2003. Lactic acid fermentation and biological activities of Rubus coreanus. J Korean Soc Agric Chem Biotechnol 46: 367-375.
  28. Kang DH, Kim JW, Youn KS. 2011. Antioxidant activities of extracts from fermented mulberry (Cudrania tricuspidata) fruit, and Inhibitory actions on elastase and tyrosinase. Korean J Food Preserv 18: 236-243. https://doi.org/10.11002/kjfp.2011.18.2.236
  29. Duke EJ, Joyce P, Ryan JP. 1973. Characterization of alternative molecular forms of xanthine oxidase in the mouse. J Biochem 131: 187-190.
  30. Jung SW, Lee NK, Kim SJ, Han DS. 1995. Screening of tyrosinase inhibitor from plants. Korean J Food Sci Technol 27: 891-896.

Cited by

  1. Antioxidative Effects of Extracts from Different Parts of Epimedium koreanum Nakai vol.45, pp.2, 2016, https://doi.org/10.3746/jkfn.2016.45.2.188
  2. Antioxidant Activity of Extracts and Fractions from Aster scaber vol.41, pp.9, 2012, https://doi.org/10.3746/jkfn.2012.41.9.1197
  3. Biological activities of Aster scaber extracts vol.23, pp.3, 2016, https://doi.org/10.11002/kjfp.2016.23.3.393
  4. Antioxidant and Anti-Inflammatory Activities of Extracts from Eugenia caryophyllata Thunb. vol.31, pp.5, 2016, https://doi.org/10.7318/KJFC/2016.31.5.481
  5. Antioxidant activities of hot-water extracts from Aster scaber by cultivation and drying methods vol.21, pp.1, 2014, https://doi.org/10.11002/kjfp.2014.21.1.82
  6. Robustaflavone Isolated from Nandina domestica Using Bioactivity-Guided Fractionation Downregulates Inflammatory Mediators vol.24, pp.9, 2019, https://doi.org/10.3390/molecules24091789
  7. Quality characteristics activities of low sugar aronia syrup added with aspartame vol.26, pp.2, 2011, https://doi.org/10.9708/jksci.2021.26.02.183
  8. [논문 철회] 말오줌나무(Sambucus sieboldiana var. pendula) 잎 유래 phenol성 물질의 생리활성 효소 저해 및 항균효과 vol.64, pp.1, 2011, https://doi.org/10.3839/jabc.2021.002