The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Diversity of Foliar Endophytic Fungi Isolated from Lindera obtusiloba in Korea

생강나무(Lindera obtusiloba)의 잎에서 분리한 내생균의 다양성

  • Kim, Chang-Kyun (Department of Biology Education, Korea National University of Education) ;
  • Eo, Ju-Kyeong (Department of Forest Science, Seoul National University) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 김창균 (한국교원대학교 생물교육과) ;
  • 어주경 (서울대학교 산림과학부) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2012.09.13
  • Accepted : 2012.09.19
  • Published : 2012.09.28
Download PDF
⟨ Previous Next ⟩

Abstract

Leaves of Lindera obtusiloba were collected from four sites in Gangwon Province, Korea. Endophytic fungi were isolated from the leaves and identified using ITS sequences of rDNA. Total twelve species belonging of endophytic fungi were identified; Alternaria alternata, Annulohypoxylon annulatum, Creosphaeria sassafras, Diaporthe eres, Discosia sp., Epicoccum nigrum, Glomerella acutata, Glomerella cingulata, Paraconiothyrium brasiliense, Pestalotiopsis neglecta, Phomopsis amygdali, Xylaria sp. The endophytic fungus, Phomopsis amygdali, was the most dominant species isolated from L. obtusiloba in this study and the fungal diversities varied in the different sites.

생강나무에 공생하는 내생균의 다양성을 확인하기 위하여, 강원도 4 개 지역에서 병증이 없는 생강나무 잎을 채집하여 내생균을 분리하였다. 분리된 내생균의 rDNA의 ITS지역을 분석한 결과, Alternaria alternata, Annulohypoxylon annulatum, Creosphaeria sassafras, Diaporthe eres, Discosia sp., Epicoccum nigrum, Glomerella acutata, Glomerella cingulata, Paraconiothyrium brasiliense, Pestalotiopsis neglecta, Phomopsis amygdali, Xylaria sp.의 총 7과 11속 12종이 확인되었으며 Phomopsis amygdali가 모든 연구 지역의 생강나무 잎에서 가장 높은 빈도로 분리되었으며 채집지에 따라 내생균의 종 다양성에 차이가 있음을 확인하였다.

Keywords

References

  1. Arnold, A., Maynard, Z. and Gilbert, G. 2001. Fungal endophytes in dicotyledonous neotropical trees: patterns of abundance and diversity. Mycol. Res. 105:1502-1507. https://doi.org/10.1017/S0953756201004956
  2. Burpee, L. and Bouton, J. 1993. Effect of eradication of the endophyte Acremonium coenophialum on epidemics of Rhizoctonia blight in tall fescue. Plant Disease 77:157-159. https://doi.org/10.1094/PD-77-0157
  3. Carroll, G. 1988. Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont. Ecology 69:2-9. https://doi.org/10.2307/1943154
  4. Chareprasert, S., Piapukiew, J., Thienirun, S., Whalley, A. J. S. and Sihanonth, P. 2006. Endophytic fungi of teak leaves Tectona grandis L. and rain tree leaves Samanea saman Merr. World J. Microb. Biot. 22:482-486.
  5. Clay, K. 1990. Fungal endophytes of grasses. Ann. Rev. Ecol. Syst. 21:275-297. https://doi.org/10.1146/annurev.es.21.110190.001423
  6. Gao, X., Zhou, H., Xu, D., Yu, C., Chen, Y. and Qu, L. 2005. High diversity of endophytic fungi from the pharmaceutical plant, Heterosmilax japonica Kunth revealed by cultivationindependent approach. FEMS Microbiol. Lett. 249:255-266. https://doi.org/10.1016/j.femsle.2005.06.017
  7. Gardes, M. and Bruns, T. 1993. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol. Ecol. 2:113-118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  8. Hwang, K. A., Shin, S. R. and Kim, K. S. 2003. Changes on the flavor components in the leaf teas of Lindera obtusiloba BL. by processing methods. Kor. J. Food Preserv. 10:488- 492. (In Korean).
  9. Hyde, K. D. and Soytong, K. 2008. The fungal endophyte dillema. Fungal Divers. 33:163-173.
  10. Kil, Y. J., Eo, J. K. and Eom, A. H. 2009. Molecular identification and diversity of endophytic fungi isolated from Pinus densiflora in Boeun, Korea. Kor. J. Mycol. Soc. 37:130-133. (In Korean). https://doi.org/10.4489/KJM.2009.37.2.130
  11. Kim, C. S., Park, M. S. and Yu, S. H. 2008. Two species of endophytic Penicillium from Pinus rigida in Korea. Mycobiology 36:222-227. https://doi.org/10.4489/MYCO.2008.36.4.222
  12. Kim, S. H., Son, J. H. and Lee, S. H. 2009. Inhibitory effects of water extract of Lindera obtusiloba on the mast cellmediated allegic inflammation. Kor. J. Pharmacogn. 40:233- 237. (In Korean).
  13. Kwon, D. J., Kim, J. K. and Bae, Y. S. 2007. Essential oils from leaves and twigs of Lindera obtusiloba. J. Kor. For. Sci. 96:65-69. (In Korean).
  14. Kwon, J. H., Kang, S. W., Kim, J. S. and Park, C. S. 2002. Anthracnose of Achyranthes japonica caused by Glomerella cingulata in Korea. Res. Plant Dis. 8:59-62. (In Korean). https://doi.org/10.5423/RPD.2002.8.1.059
  15. Park, C. W. 2005. The genera of vascular plants of Korea. Academy Publishing, Seoul.
  16. Photita, W., Taylor, P. W. J., Ford, R., Hyde, K. D. and Lumyong, S. 2005. Morphological and molecular characterization of Colletotrichum species from herbaceous plants in Thailand. Fungal Divers. 18:117-133.
  17. Rubini, M., Silva-Ribeiro, R., Pomella, A., Maki, C., Araujo, W., dos Santos, D. and Azevedo, J. 2005. Diversity of endophytic fungal community of cacao (Theobroma cacao L.) and biological control of Crinipellis perniciosa, causal agent of Witches' Broom Disease. Int. J. Biol. Sci. 1:24-33.
  18. Seo, K. H., Baek, M. Y., Lee, D. Y., Cho, J. G., Kang, H. C., Ahn, E. M., Baek, N. I. and Lee, Y. H. 2011. Isolation of flavonoids and lignans from the stem wood of Lindera obtusiloba Blume. J. appl. Biol. Chem. 54:178-183. (In Korean). https://doi.org/10.3839/jabc.2011.030
  19. Seo, S. T., Kim, K. H., Kim, M. J., Hong, J. S., Park, J. H. and Shin, S. C. 2009. Diversity of fungal endophytes from Pinus koraiensis leaves in Korea. Kor. J. Mycol. 37:108-110. (In Korean). https://doi.org/10.4489/KJM.2009.37.1.108
  20. Sreekanth, D., Sushim, G. K., Syed, A., Khan, B. M. and Ahmad, A. 2011. Molecular and morphological characterization of a taxol-producing endophytic fungus, Gliocladium sp., from Taxus baccata. Mycobiology 39:151- 157. https://doi.org/10.5941/MYCO.2011.39.3.151
  21. Wei, J. G., Xu, T., Guo, L. D., Liu, A. R., Zhang, Y. and Pan, X. H. 2007. Endophytic Pestalotiopsis species associated with plants of Podocarpaceae, Theaceae and Taxaceae in southern China. Fungal Divers. 24:55-74.
  22. Wong, M. K. M. and Hyde, D. M. 2001. Diversity of fungi on six species of Gramineae and one species of Cyperaceae in Hong Kong. Mycol. Res. 105:1485-1491. https://doi.org/10.1017/S0953756201004695

Cited by

  1. New evidence for broad trophic status of leaf endophytic fungi of Quercus gambelii vol.22, 2016, https://doi.org/10.1016/j.funeco.2016.04.003
  2. Diversity of Endophytic Fungi Isolated from Korean Ginseng Leaves vol.42, pp.2, 2014, https://doi.org/10.5941/MYCO.2014.42.2.147
  3. Diversity and Seasonal Variation of Endophytic Fungi Isolated from Three Conifers in Mt. Taehwa, Korea vol.41, pp.2, 2013, https://doi.org/10.5941/MYCO.2013.41.2.82
  4. Diversity of Endophytic Fungi Isolated from Leaves of Coniferous Trees on Mt. Minjuji, Korea vol.42, pp.2, 2014, https://doi.org/10.4489/KJM.2014.42.2.174
  5. Molecular Identification of Endophytic Fungi Isolated from Needle Leaves of Pinus thunbergii vol.40, pp.4, 2012, https://doi.org/10.4489/KJM.2012.40.4.183
  6. A Note on a Dark Septate Endophyte Phialocephala piceaeIsolated from Needle Leaves of Thuja koraiensis in Korea vol.44, pp.4, 2016, https://doi.org/10.4489/KJM.2016.44.4.338
  7. Molecular Identification of Endophytic Fungi Isolated from Needle Leaves of Conifers in Bohyeon Mountain, Korea vol.40, pp.4, 2012, https://doi.org/10.5941/MYCO.2012.40.4.231
  8. Identification and Characterization of Paraconiothyrium brasiliense from Garden Plant Pachysandra terminalis vol.42, pp.4, 2014, https://doi.org/10.4489/KJM.2014.42.4.262
  9. Diversity of Endophytic Fungi Isolated from Pinus densiflora and Larix kaempferi in Mt. Oser, Korea vol.41, pp.3, 2013, https://doi.org/10.4489/KJM.2013.41.3.137