The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Isolation and Identification of Yeasts from Wild Flowers of Daejeon City and Chungcheongnam-do in Korea

대전과 충남 지역에서 채취한 야생화로부터 효모의 분리 및 동정

  • Min, Jin-Hong (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Hyun, Se-Hee (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Kang, Min-Gu (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Hyang-Beom (Divison of Applied Bioscience & Biotechnology, Chonnam National University) ;
  • Kim, Chang-Mu (National Institute of Biological Resources, Korea(Environmental Research Complex)) ;
  • Kim, Ha-Kun (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University)
  • 민진홍 (배재대학교 바이오.의생명공학과) ;
  • 현세희 (배재대학교 바이오.의생명공학과) ;
  • 강민구 (배재대학교 바이오.의생명공학과) ;
  • 이향범 (전남대학교 응용생명공학부) ;
  • 김창무 (국립생물자원관) ;
  • 김하근 (배재대학교 바이오.의생명공학과) ;
  • 이종수 (배재대학교 바이오.의생명공학과)
  • Received : 2012.09.10
  • Accepted : 2012.09.15
  • Published : 2012.09.28
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Abstract

Total 50 species, 80 strains: Eighty strains of yeasts were isolated from wild flowers of Daejeon city and its surroundings in Korea. Isolated yeasts were identified by comparison of their PCR-amplified 18s rDNA V3 region or internal transcribed spacer-2 (ITS-2) genes with BLAST database. Among them, 32 species, 54 strains of yeasts were isolated and identified from wild flowers of Daejeon city, and Cryptococcus species were dominant yeasts. Furthermore, 18 species, 26 strains of yeasts were isolated and identified from wild flowers of Geumsan-gun and Nonsan-gun, Chungcheongnam-do in Korea, and also Candida species and Pseudozyma species were dominant yeasts.

자연환경에서 분포하고 있는 효모의 mycoflora를 확인하고 궁극적으로 이들을 고부가가치의 산업에 응용하기 위한 연구의 일환으로 대전과 인근 충남지역 일부의 야생화들을 채집하여 효모들을 분리, 동정하였다. 대전지역 야생화 42점에서 32종의 효모들을 54균주 분리하였고 이들 중 Cryptococcus 속균과 Phodotonula 속균 등이 우점균이었으며 Metschnikowia 속균과 같이 희귀 효모들을 20여종 분리하였다. 대전 인근의 충남 금산군과 논산군 일부지역의 야생화 28점에서 모두 18종의 효모들을 26균주 분리하였다. 이들 균 중에서 Candida 속균과 Pseudozyma 속균 등이 우점균으로 분리되었다.

Keywords

References

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